Monosaccharides were analyzed as their alditol acetates by GC-MS. Methylation was performed on the isolated PS according to the method described by Hakomori [41 (link)]. Alditol acetates and partially methylated alditol acetates were analyzed by GC-MS using an ITQ 700 (Thermo Scientific, Waltham, MA, USA) system, equipped with a HP-1 fused-silica capillary column (0.2 mm × 12.5 m) and a temperature gradient from 150 to 270 °C at 12 °C·min−1. The absolute configurations of the sugars were determined as described by Gerwig et al. [42 (link)] using (−)-2-butanol for the formation of 2-butyl glycosides. The trimethylsilylated butyl glycosides were then identified by comparison with authentic samples on GC-MS.
Itq 700
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ITQ 700 is a high-performance ion trap mass spectrometer designed for demanding analytical applications. It features a compact design, robust construction, and advanced technology to provide reliable and accurate results. The core function of the ITQ 700 is to perform sensitive and selective mass spectrometry analysis of a wide range of samples.
Automatically generated - may contain errors
5 protocols using itq 700
1
Structural Analysis of Monosaccharides
2
Determining Cellular Lipid Content and Fatty Acid Profiles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total lipid content of the cells was determined in triplicate by the modified Bligh & Dyer [23 (link)] method using 1 : 2 chloroform : methanol and presented as weight percentage (%) [23 (link)]. Fatty acids were determined as fatty acid methyl esters (FAMEs) in triplicate after acidic transesterification of lipids [24 ]. The resulting FAMEs were analysed by gas chromatograph mass spectrometry (GC-MS; Thermo Scientific ITQ 700, USA) equipped with a flame ionization detector (FID) and a fused silica capillary column (60 m × 0.25 mm × 0.25 µm; Agilent Technologies, USA). The injector and detector temperatures were maintained at 270 and 280°C, respectively, with an oven temperature gradient of 50–170°C at 40°C min−1 after a 1 min hold time at 50°C, then with an oven temperature gradient of 170–210°C at 18°C min−1 after a 1 min hold. All parameters of the FAME were derived from the calibration curves generated from the FAME standard mix (Supelco 37 component FAME mix, Sigma-Aldrich, USA) [25 (link)].
3
Fatty Acid Composition Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The FAME procedure described previously [16 (link)] was applied, using a Thermo Scientific ITQ 700 (Thermo Fisher Scientific) gas chromatograph-ion trap spectroscopy equipped with PEG columns (30 m X 0.25 mm id., 0.25 μm thickness) (DB-FFAP Agilent Technologies, France. Data collection and processing were performed by means of XCALIBUR software, (version 2.0 Thermo Fisher Scientific). The relative amount of each fatty acid (% of total fatty acids) was determined by integrating the area under the peak and dividing the result by the total area for all the fatty acid peaks present in the sample.
4
Characterization of Equisetum canadensis FF
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The influence
of the UAE process
of the separation of E. canadensis functional
FF on the overall chemical properties was analyzed using a variety
of colorimetric and spectroscopic techniques, described previously.26 (link) All colorimetric assays were performed with
a SPECTROStar UV–vis spectrophotometer (BMG LABTECH, Germany).
The contents of saccharides, uronic acids, and total phenolics were
estimated by the phenol–sulfuric, m-hydroxybiphenyl,
and Folin–Ciocalteu assay, respectively.25 (link) The monosaccharide composition was determined by the gas
chromatography-mass spectrometry (GC-MS) technique (Trace GC Ultra
chromatograph with an ion trap detector ITQ 700, Thermo Scientific,
equipped with an Agilent HP-88 column (0.25 mm × 30 m), where
the mixture of the neutral monosaccharides was obtained from the corresponding
bioproducts after hydrolysis with TFA (trifluoroacetic acid, 2 mol/L),
120 °C, 5 h) and derived to obtain the volatile alditol acetate
forms, prior to the analysis (seeText S4 , Supporting Information).25 (link) The separation
of the horseweed FF obtained through the optimized UAE procedure was
conducted with the GPC technique on a glass column (15 mm × 1500
mm) packed with a Sephacryl 300 HR gel (Sigma-Aldrich, Germany) with
aqueous 0.1 mol/L NaOH eluent, followed by determination of the molecular
weights of the obtained fractions.
of the UAE process
of the separation of E. canadensis functional
FF on the overall chemical properties was analyzed using a variety
of colorimetric and spectroscopic techniques, described previously.26 (link) All colorimetric assays were performed with
a SPECTROStar UV–vis spectrophotometer (BMG LABTECH, Germany).
The contents of saccharides, uronic acids, and total phenolics were
estimated by the phenol–sulfuric, m-hydroxybiphenyl,
and Folin–Ciocalteu assay, respectively.25 (link) The monosaccharide composition was determined by the gas
chromatography-mass spectrometry (GC-MS) technique (Trace GC Ultra
chromatograph with an ion trap detector ITQ 700, Thermo Scientific,
equipped with an Agilent HP-88 column (0.25 mm × 30 m), where
the mixture of the neutral monosaccharides was obtained from the corresponding
bioproducts after hydrolysis with TFA (trifluoroacetic acid, 2 mol/L),
120 °C, 5 h) and derived to obtain the volatile alditol acetate
forms, prior to the analysis (see
of the horseweed FF obtained through the optimized UAE procedure was
conducted with the GPC technique on a glass column (15 mm × 1500
mm) packed with a Sephacryl 300 HR gel (Sigma-Aldrich, Germany) with
aqueous 0.1 mol/L NaOH eluent, followed by determination of the molecular
weights of the obtained fractions.
5
Methanolysis and GC-MS Analysis of Fatty Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The methanolysis of FAs was performed with 1 M H 2 SO 4 -CH 3 OH at 100°C for 1 h using the modified Davila method [6] . FA components of extracted lipid were analyzed by gas chromatography mass spectrometry (Ultra trace, Thermo Scientific ITQ 700, USA) equipped with a fused silica capillary column (60 mm × 0.25 mm × 0.25 µm; Agilent Technologies, USA) and a flame ionization detector (FID). The initial temperature was maintained at 50°C for 1 min and then increased to 170°C at 40°C/min. The oven temperature was raised from 170°C to 210°C at 18°C/min after a 1 min hold. The injector temperature was 270°C, and nitrogen was used as a carrier gas with a flow rate of 2.0 ml/min. The detector temperature was 280°C, and air and hydrogen flows were set at 350 and 35 ml/min, respectively. All the parameters of FA methyl ester (FAME) were derived from the calibration curves generated from the FAME standard mix (Supelco 37 Component FAME Mix; Sigma-Aldrich, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!