Cells (NB4 and U937-PR9) were washed twice in phosphate-buffered saline (PBS) and lysed on ice for 30 min in RIPA buffer. Protein extracts were separated in a sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) gel. The proteins were then transferred to a polyvinylidenedifluoride (PVDF) membrane and probed with anti-RARα (C-20; Santa Cruz Biotechnology) and anti-β-actin (Sigma-Aldrich) antibodies.
Anti rarα c 20
Manufactured by Santa Cruz Biotechnology
Anti-RARα (C-20) is a polyclonal antibody that recognizes the C-terminus of the human retinoic acid receptor alpha (RARα) protein. RARα is a member of the nuclear receptor superfamily and plays a role in gene regulation.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Merck Group (1 mentions)
Sorafenib, by Selleck Chemicals (1 mentions)
Sb415286, by Selleck Chemicals (1 mentions)
Anti cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions)
Tideglusib, by Selleck Chemicals (1 mentions)
Anti ki 67 8d5, by Cell Signaling Technology (1 mentions)
Anti gapdh fl 335, by Santa Cruz Biotechnology (1 mentions)
Anti phospho gsk 3βser9 5b3, by Cell Signaling Technology (1 mentions)
Lipofectamin 2000, by Thermo Fisher Scientific (1 mentions)
Anti flag m2, by Merck Group (1 mentions)
2 protocols using anti rarα c 20
1
Western Blot Analysis of RARα
2
Immunoblotting analysis of GSK-3β and apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies include: anti-GSK-3β (D5C5Z, #12456), anti-phospho-GSK-3β (Ser9,5B3) (#9323) anti-Ki-67 (8D5, #9449) anti-Cleaved Caspase-3 (Asp175) (#9661) from Cell Signaling Technology (CST); anti-phospho-serine/threonine (ab15556) and anti-phospho-serine (ab9332) from Abcam; anti-Flag® M2 from Sigma-Aldrich; anti-RARB (A1603) from ABclonal; anti-PARP1 (66520-1-Ig) and anti-His (66005-1-Ig) from Proteintech; anti-rabbit and anti-mouse secondary antibodies conjugated to horseradish peroxidase, anti-rabbit secondary antibodies conjugated to Cy3 from Invitrogen; anti-RXRα (ΔN197, sc-774; D20, sc-553), anti-RARα (C20, sc-551), anti-GFP (B-2,sc-9996), anti-c-Myc (9E10, sc-40), anti-β-actin (H196, sc-7210) and anti-GAPDH (FL-335, sc-25778) from Santa Cruz Biotechnology. Chemicals and other regents are: lipofectamin 2000 from Invitrogen; enhanced chemiluminescence (ECL), protein A/G agarose from ThermoFisher Scientific; sorafenib, BIO, SB415286 and tideglusib from Selleck Chemicals; 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), 4,6-Diamidino-2-phenylindole (DAPI), 9-cis-RA, isopropyl-1-thio-b-D-galactopyranoside (IPTG) and LiCl from Sigma-Aldrich; cocktail of proteinase and phosphatase inhibitors from Roche; Dual-Luciferase Assay System Kit from Promega.
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