Male C57BL/6 mice, 6 to 7 weeks old, were obtained from Harlan Laboratories (Indianapolis, IN). Hamster antimouse CD3ε and antimouse CD28 were obtained from BD Biosciences (San Diego, CA). Rat affinity purified antimouse CD16/32, hamster PE-conjugated antimouse CD11c, rat FITC-conjugated antimouse MHC II, rat APC-conjugated antimouse F4/80, hamster PE-Cy7-conjugated antimouse CD3ε, and recombinant IL-12 and IL-23 were obtained from eBioscience (San Diego, CA). Goat APC-conjugated antimouse IL-23R and IL-17 and IL-22 enzyme-linked immunosorbent assay (ELISA) kits were obtained from R&D Systems (Minneapolis, MN). Collagenase D was obtained from Roche Applied Science (Indianapolis, IN). Concanavalin A (ConA), ionomycin calcium salt, phorbol 12-myristate 13-acetate (PMA), and AhR inhibitor CH-223191 were obtained from Sigma-Aldrich (St. Louis, MO).
Hamster antimouse cd3ε
Manufactured by BD
Hamster antimouse CD3ε is a laboratory reagent used in flow cytometry and other immunological applications. It is a monoclonal antibody that specifically binds to the CD3ε chain of the T cell receptor complex in mice. The product is intended for research use only.
Automatically generated - may contain errors
2 protocols using hamster antimouse cd3ε
1
Murine T-Cell Activation and Cytokine Production
2
Quantifying T Cell Proximity in Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cre reporter mice were inoculated with II-Cre as described above. At 21 dpi, mice were perfused with cold PBS followed by 4% PFA and drop fixed in 4% PFA overnight before being transferred to 30% sucrose solution. Brains were sectioned to 200 μm on a vibratome and processed using the PACT clearing technique described elsewhere (77 (link)). After clearing, using the previously described microwave protocol (78 (link)), brain sections were stained with anti-CD3ε antibodies (hamster anti-mouse CD3ε; BD Biosciences; catalog no. 550277), followed by an appropriate secondary (goat anti-hamster Alexa Fluor 647 [Invitrogen; catalog no. A21451]), and NeuroTrace 435/455 fluorescent Nissl.
Images were captured on a Zeiss 880 NLO upright microscope, with a 20× objective, and imported into Bitplane Imaris software. Within Imaris, the spots feature was used to represent the cell body of each respective cell of interest, TINs, Bystanders, and T cells, based upon fluorescent staining. X,Y,Z positions from each spot were exported into Matlab to calculate the distances of each T cell to either Bystanders or TINs. T cells within 20 μm of a TIN or Bystander were used for analysis.
Images were captured on a Zeiss 880 NLO upright microscope, with a 20× objective, and imported into Bitplane Imaris software. Within Imaris, the spots feature was used to represent the cell body of each respective cell of interest, TINs, Bystanders, and T cells, based upon fluorescent staining. X,Y,Z positions from each spot were exported into Matlab to calculate the distances of each T cell to either Bystanders or TINs. T cells within 20 μm of a TIN or Bystander were used for analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!