Fluvastatin sodium was kindly donated by Eipico (Cairo, Egypt), Span 60 (sorbitan monostearate), Span 80 (sorbitan monooleate), Tween 80 (polyoxyethylene sorbitan monooleate), Brij 35 (polyoxyethylene lauryl ether), acetonitrile (HPLC grade), methanol (HPLC grade), diethyl ether (HPLC grade), complete Freund's adjuvant (CFA), and methotrexate were acquired from Sigma-Aldrich (St. Louis, MO). Dialysis bags with a molecular weight cut off of 12,000 Da were obtained from SERVA Electrophoresis GmbH (Heidelberg, Germany). Serum rheumatoid factor (RF), cartilage oligomeric matrix protein (COMP), and antinuclear antibody (ANA) ELISA kits were purchased from CliniLab Company (Cairo, Egypt). Serum tumor necrosis factor-alpha (TNF-α) ELISA kit was obtained from Glory Science company (St. Del Rio, TX). Serum interleukin-10 (IL-10) ELISA kit was obtained from Cusabio Biotech Co. (Wuhan, China). Serum malondialdehyde (MDA) and glutathione (GSH) reduced colorimetric kits were purchased from Biodiagnostic Company (Giza, Egypt). Acrylamide Kit (SDS-PAGE) (for sodium dodecyl sulfate polyacrylamide gel electrophoresis) was provided by Bio-Rad Laboratories (TNC, Hercules, CA) catalog no. 161-0181. All other ingredients used were of analytical grade.
Fluvastatin sodium
Manufactured by Merck Group
Fluvastatin sodium is a synthetic statin medication used to lower cholesterol levels. It functions by inhibiting the enzyme HMG-CoA reductase, which plays a key role in the production of cholesterol in the body.
Automatically generated - may contain errors
Lab products found in correlation
Methotrexate, by Merck Group (1 mentions)
Dialysis bag, by Serva Electrophoresis (1 mentions)
Complete freund s adjuvant cfa, by Merck Group (1 mentions)
Brij 35, by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Span 60, by Merck Group (1 mentions)
Span 80, by Merck Group (1 mentions)
Lovastatin, by Merck Group (1 mentions)
Atorvastatin, by Cayman Chemical (1 mentions)
Sodium dichloroacetate, by Merck Group (1 mentions)
Rosuvastatin calcium, by Merck Group (1 mentions)
Pitavastatin, by Cayman Chemical (1 mentions)
Simvastatin, by Merck Group (1 mentions)
Coenzyme q10, by Merck Group (1 mentions)
Nutlin 3, by Selleck Chemicals (1 mentions)
C2c12 cell line, by American Type Culture Collection (1 mentions)
Oligomycin a, by Merck Group (1 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Ggti 2133, by Merck Group (1 mentions)
3 protocols using fluvastatin sodium
1
Development and Evaluation of Fluvastatin Nanoformulations for Rheumatoid Arthritis Treatment
2
Rhabdomyosarcoma and myoblast cell culture with statins
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The human rhabdomyosarcoma cell line A-204 and the mouse myoblast C2C12 cell line were obtained from ATCC, and maintained in McCoy's 5A medium (Gibco) and Dulbecco's modified Eagle's medium (Gibco) respectively. C2C12 myoblasts were stimulated to differentiate into myotubes by switching to the medium with 2% horse serum. The human colon cancer HCT116 SCO2−/− cell line was a generous gift from Dr. Paul M. Hwang at the National Institutes of Health, and cultured in McCoy's 5A medium.
Lovastatin, fluvastatin sodium, simvastatin, rosuvastatin calcium, mevalonic acid sodium, sodium dichloroacetate, coenzyme Q10 (CoQ10) and oligomycin A were purchased from Sigma Aldrich. Pitavastatin and atorvastatin were purchased from Cayman Chemical. Nutlin-3 was purchased from Selleck Chemicals. The doses of statins in cell culture were consistent with previous studies in SAMS [18 (link)].
Lovastatin, fluvastatin sodium, simvastatin, rosuvastatin calcium, mevalonic acid sodium, sodium dichloroacetate, coenzyme Q10 (CoQ10) and oligomycin A were purchased from Sigma Aldrich. Pitavastatin and atorvastatin were purchased from Cayman Chemical. Nutlin-3 was purchased from Selleck Chemicals. The doses of statins in cell culture were consistent with previous studies in SAMS [18 (link)].
3
Inhibition of Protein Prenylation Pathways
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Fluvastatin sodium (Sigma), the prenylation enzyme inhibitor (geranylgeranyl transferase inhibitor (GGTI-2133, Sigma), farnesyl pyrophosphate transferase inhibitor (FPTI III, Santa Cruz biotechnologies)) and the squalene synthase inhibitor zaragozic acid A (Sigma) were dissolved in DMSO and stored at −20 °C. Cholesterol (Sigma), mevalonic acid (Sigma) and geranylgeranyl pyrophosphate (GGPP, Sigma) were dissolved in absolute ethanol and stored at −20 °C. Farnesyl pyrophosphate (FPP, Sigma) was dissolved in methanol and stored at −20 °C.
Stock solutions were diluted in cell culture media to a final concentration of DMSO of 0.1% and ethanol or methanol of 0.2%.
Stock solutions were diluted in cell culture media to a final concentration of DMSO of 0.1% and ethanol or methanol of 0.2%.
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