Ldlr-/- mice were obtained from Jackson Laboratory (Bar Harbor, ME, USA). The mice were bred in a specific pathogen-free facility under 12 h light and 12 h dark cycle, with free access to food and water. Fifteen male Ldlr-/- mice (10 week old) were fed a western diet (WD) from Test Diet (AIN-76A; St. Louis, MO, USA) for 10 weeks. All animal experiments were performed with the approval of the Institutional Animal Care and Use Committee of Hanyang University, Seoul, Korea.
Ain 76a
Manufactured by TestDiet
Sourced in United States
The AIN-76A is a type of laboratory equipment designed for use in scientific research and analysis. It is a purified and chemically defined diet for rodents. The core function of AIN-76A is to provide a controlled and standardized nutritional environment for animal studies.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6j mice, by Jackson ImmunoResearch (2 mentions)
Ldlr mice, by Jackson ImmunoResearch (1 mentions)
Apoe b6.129p2 apoetm1unc j, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Lieber decarli diet, by Bio-Serv (1 mentions)
C57bl 6j mice, by Charles River Laboratories (1 mentions)
C57 bl6 mice, by TestDiet (1 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
P53fl fl mice, by Jackson ImmunoResearch (1 mentions)
Fxr mice, by Jackson ImmunoResearch (1 mentions)
Aav8 tbg cre, by Vector Biolabs (1 mentions)
Ain76a diet, by TestDiet (1 mentions)
C57bl 6 wt mice, by Charles River Laboratories (1 mentions)
16 protocols using ain 76a
1
Western Diet-Induced Atherosclerosis in Ldlr-/- Mice
2
T-Maze Spatial Working Memory Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The test of working memory was performed in an automated T-maze (O’Hara & Co., Ltd., Japan) as previously described (Shoji et al., 2012 (link)). Briefly, after food restriction (to 70% of normal consumption), we habituated the animals to the apparatus by placing them in the starting chamber for 10 min on two consecutive days. Each trial of the forced alternation task consisted of two runs: on the first (sample) run, the animals were forced to go into one arm of the T-maze to receive a food pellet (10 mg, colorless, odorless, round, AIN-76A, #1811213, TestDiet, St. Louis, MO, USA) as a reward; on the second (choice) run, both arms were open, but the reward was only available in the previously non-visited arm. During the first phase (learning), the animals underwent 10 trials per day without a delay between the sample and choice runs. When an animal’s performance reached a criteria of 80% correct choices per day, the 2nd phase (delayed alternation) was initiated with 0, 10, 30, 60 and 120 s delays between trials (10 trials for each interval in a pseudorandom order tested on five consecutive days).
3
Foz/Foz Mice in Metabolic Disorders
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice with the Alms1 mutation (Foz/Foz mice) on C57BL6/J and a Non-obese Diabetic (NOD) genetic background were kindly provided by Dr Geoffrey C. Farrell (Australian National University Medical School).8 (link) Six- to 7-week-old (time point = 0 w) male and female Foz/Foz along with WT littermates were placed on a WD (AIN-76A; Test Diet, St. Louis, MO, containing 40% fat, 15% protein, 44% carbohydrates based on caloric content, 0.2% cholesterol) or a standard chow diet (12% fat, 23% protein, 65% carbohydrates) for up to 32 weeks. For the regression study, Foz+WD 12-week mice were switched to the standard chow diet for an additional 8–12 weeks. A separate cohort of 6-week-old C57BL6/J Foz/Foz mice was placed on a CD-HFD (D-05010402; Research Diet, New Brunswick, NJ, containing 45% fat, 20% protein, 35% carbohydrates) for 12 weeks. Because Foz/Foz mice are hyperphagic, all mice were fasted for 4 hours before death and tissue collection. All animals were maintained under pathogen-free conditions in filter-topped cages on autoclaved food and water and under a 12/12-hour light/dark cycle, in accordance with National Institutes of Health guidelines for the use and care of live animals and approved by the University of California San Diego Institutional Animal Care and Use Committee (IACUC, Protocol #S07022).
4
Apo-E and miR-155 Knockout Mice in Diet-Induced Atherosclerosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal experiments were performed in accordance with the Institutional Animal Care and Use Committee (IACUC) guidelines and were approved by the IACUC of Lewis Katz School of Medicine (LKSOM) at Temple University. Apolipoprotein E (ApoE, B6.129P2-ApoEtm1Unc/J, stock no. 002052) knockout mice, microRNA-155 (miR-155, B6.Cg-Mir155tm1Rsky/J, stock no. 007745) knockout mice, and wild-type (WT) mice were of a C57BL/6J background, and were purchased from the Jackson Laboratory (Bar Harbor, ME, United States). Mice were housed under controlled conditions in the LKSOM Animal Facility, where they had ad libitum access to standard chow diet/HFD, water, and were subject to a 12-h light-dark cycle. DKO mice were generated as previously reported (11 (link)) by crossing ApoE-/- mice with miR155-/- mice. Mice were age-matched and gender-specific in all experiment groups, unless otherwise stated. At eight weeks old, mice either remained on normal chow diet (10.7% fat, 23.9% protein, 5.1% fiber, 58.7% carbohydrate/other, 200ppm cholesterol; Labdiet 5001) or switched to HFD [20% (w/w) fat, 17.4% protein, 5% fiber, 49.9% carbohydrate/other, 2027 ppm cholesterol (0.15% (w/w) cholesterol); TestDiet AIN-76A] (34 (link)) for 12 weeks or 24 weeks, specified in each experiment.
5
High-Fat/High-Cholesterol Diet in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Twelve‐week‐old male C57BL/6J mice were purchased from the Jackson Laboratory (Bar Harbor, ME). Mice were fed for 16 weeks a high‐fat/high‐cholesterol/high‐fructose diet (Western diet) containing 40% fat/0.2% cholesterol (AIN‐76A; TestDiet, St. Louis, MO) and 4.2% fructose (in drinking water). The Lieber‐DeCarli diet was purchased from Bio‐Serv (Flemington, NJ). All of the animals received human care according to the criteria outlined in the “Guide for the Care and Use of Laboratory Animals” prepared by the National Academy of Sciences and published by the National Institutes of Health. Mice were fasted for 5 to 6 hours before euthanasia during the light cycle. All of the animal studies were approved by the Institutional Animal Care and Use Committee at Northeast Ohio Medical University.
6
Murine Diabetes Induction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal procedures were approved by the British Home Office under the UK Animal (Scientific Procedures) Act 1986 (Project Licence 70/7596). Male C57BL/6 J mice (8–10 weeks, Charles River) were maintained at 21–23 °C and light-dark cycles (12:12 h schedule, lights on at 07:00). Ad libitum access to water and normal chow (RM1, Special Diet Services) or a HFHS diabetogenic diet (AIN-76A, TestDiet) was provided unless otherwise stated. HFHS animals were initially group housed (5 per cage) for >4 months before transfer to single cages. Treatments were randomly allocated according to body weight. Group sizes of 8–10 were deemed adequate to detect treatment-related differences as per initial dose-finding glycaemia. During experiments, one researcher was aware of treatment allocation but others were blinded. No animals were excluded from the analysis.
7
Transfer Learning Task Apparatus and Procedures
8
Rodent Operant Chambers for Behavioral Research
9
Sucrose Consumption in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were given a sucrose eating challenge to determine whether free access to sucrose would produce different levels of consumption between the genotypes. For these sessions the mice were given 95% sucrose pellets (AIN-76A, TestDiet, Richmond, IN) for 10 hours per day for 4 days, and normal lab chow for 14 hours. The amount of sucrose and chow consumed was measured daily by weight of 5 mg sucrose pellets/chow consumed and expressed as g/kg of bodyweight.
10
Sucrose Self-Administration in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Parameters for sucrose self-administration were identical to those for nicotine with three exceptions: (1) rats did not undergo surgery; (2) active nose-pokes were reinforced with a 45 mg sucrose pellet (AIN-76A; TestDiet, MO, USA) delivered into the magazine; and (3) sessions lasted until a maximum of 30 pellets were earned or 30 minutes had elapsed.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!