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Sequencing grade endoproteinase lys c

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Sequencing-grade endoproteinase Lys-C is a highly specific serine protease that cleaves peptide bonds at the carboxyl side of lysine residues. It is suitable for the preparation of peptide samples for sequencing analysis.

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Lab products found in correlation

Sequencing grade trypsin, by Promega (3 mentions) Formic acid, by Merck Group (3 mentions) Ammonium bicarbonate, by Merck Group (3 mentions) Sodium dodecyl sulfate (sds), by Merck Group (3 mentions) Lc ms grade acetonitrile acn, by Avantor (2 mentions) Glycerol free pngase f, by New England Biolabs (2 mentions) Triethylammonium bicarbonate, by Merck Group (1 mentions) Ms grade acetonitrile, by Avantor (1 mentions) Glycerol free peptide n glycosidase f, by New England Biolabs (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Iodoacetamide, by Merck Group (1 mentions)

Spelling variants of this product

Lys-C (9 citations) Endoproteinase Lys-C (7 citations)

3 protocols using sequencing grade endoproteinase lys c

1

Protein Sample Preparation for LC-MS

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LC-MS-grade acetonitrile (ACN) and water were obtained from Burdick & Jackson (Muskegon, MI). Reagents for protein chemistry, including sodium dodecyl sulfate (SDS), ammonium bicarbonate, iodoacetamide (IAA), dithiothreitol (DTT), sequencing-grade endoproteinase Lys-C, and formic acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO). Sequencing-grade trypsin was purchased from Promega (Madison, WI). Glycerol-free PNGase F was purchased from New England BioLabs (Ipswich, MA).
Bons J., Pan D., Shah S., Bai R., Chen-Tanyolac C., Wang X., Elliott D.R., Urisman A., O’Broin A., Basisty N., Rose J., Sangwan V., Camilleri-Broët S., Tankel J., Gascard P., Ferri L., Tlsty T.D, & Schilling B. (2022). Data-independent acquisition and quantification of extracellular matrix from human lung in chronic inflammation-associated carcinomas. Proteomics, 23(7-8), e2200021.
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2

Protein Sample Preparation for Mass Spectrometry

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Liquid chromatography–MS grade acetonitrile (ACN), methanol, and water were obtained from Burdick & Jackson (Muskegon, MI). Reagents for protein chemistry, including SDS, ammonium bicarbonate, triethylammonium bicarbonate (TEAB), iodoacetamide, DTT, sequencing-grade endoproteinase Lys-C, and formic acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO). Sequencing-grade trypsin was purchased from Promega (Madison, WI). Glycerol-free Peptide:N-glycosidase F was purchased from New England BioLabs (Ipswich, MA).
Moutin E.B., Bons J., Giavara G., Lourenco F., Pan D., Burton J.B., Shah S., Colombé M., Gascard P., Tlsty T., Schilling B, & Winton D.J. (2024). Extracellular Matrix Orchestration of Tissue Remodeling in the Chronically Inflamed Mouse Colon. Cellular and Molecular Gastroenterology and Hepatology, 17(4), 639-656.
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3

Protein Sample Preparation for LC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols
LC-MS-grade acetonitrile (ACN) and water were obtained from Burdick & Jackson (Muskegon, MI). Reagents for protein chemistry, including sodium dodecyl sulfate (SDS), ammonium bicarbonate, iodoacetamide (IAA), dithiothreitol (DTT), sequencing-grade endoproteinase Lys-C, and formic acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO). Sequencing-grade trypsin was purchased from Promega (Madison, WI). Glycerol-free PNGase F was purchased from New England BioLabs (Ipswich, MA).
Budi S.W., van Tuinen D., Martinotti G, & Gianinazzi S. (1999). Isolation from the Sorghum bicolor Mycorrhizosphere of a Bacterium Compatible with Arbuscular Mycorrhiza Development and Antagonistic towards Soilborne Fungal Pathogens. Applied and Environmental Microbiology, 65(11), 5148-5150.
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