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Vectastain rabbit abc kit

Manufactured by Vector Laboratories
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Sourced in United Kingdom

The Vectastain Rabbit ABC kit is a laboratory product designed to facilitate immunohistochemical detection. It contains the necessary components for a streptavidin-biotin-based detection system, including a biotinylated secondary antibody and an avidin-biotin enzyme complex. The kit is intended for use with rabbit primary antibodies.

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Lab products found in correlation

Pa3 214, by Merck Group (2 mentions) Ab15134, by Merck Group (2 mentions) Hpa006889, by Merck Group (2 mentions) Methanol, by Merck Group (1 mentions) Trypsin, by Merck Group (1 mentions) Anti gfp ab290, by Abcam (1 mentions) Dp70 digital camera, by Olympus (1 mentions) Bx41 microscope, by Olympus (1 mentions)

Close spelling variants of this product

Vectastain ABC-AP Rabbit IgG Kit Vectastain ABC rabbit IgG kit Vectastain Elite ABC kit for rabbit Vectastain anti-rabbit Ig ABC-AP kit VECTASTAIN Elite ABC Rabbit IgG Kit Vectastain elite ABC rabbit kit VECTASTAIN Elite ABC Rabbit Immunoglobulin G Kit Vectastain ABC kit Vectastain ABC Vectastain kit

4 protocols using vectastain rabbit abc kit

1

Immunohistochemical Analysis of Ovarian Peptides

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Ovaries obtained after controlled ovarian stimulation were sectioned for immunostaining. Immunostaining was performed on paraffin-embedded tissues sectioned at 5 μm as previously described.18 (link) Immunodetection of NTS and NTSR2 were performed after acidic antigen retrieval (10 mM sodium citrate, 0.05% Tween20; 18), and SORT immunodetection was performed after basic antigen retrieval (10 mM Tris base, 1 mM EDTA, 0.05% Tween20; 18). Slides were blocked, then incubated overnight with rabbit primary antibodies against NTS (ImmunoStar #20072; 1:100 dilution), NTSR1 (Thermo Fisher #PA3–214; 1:400 dilution), NTSR2 (EMD Millipore #AB15134; 20 μg/mL), or SORT1 (Sigma #HPA006889; 4 μg/mL). Omission of the primary antibody served as a negative control. Slides were incubated with DAB substrate using the Vectastain Rabbit ABC kit (Vector Laboratories, Burlingame, CA) according to manufacturer instructions, then counterstained in hematoxylin.
Campbell G.E., Bender H.R., Parker G.A., Curry TE J.r, & Duffy D.M. (2021). Neurotensin: A novel mediator of ovulation?. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(4), e21481.
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2

Histological Analysis of Murine Tibiae

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Tibiae were dissected from 6‐week‐old male WT (n = 6) and Socs2‐/‐ mice (n = 6) and fixed in 70% ethanol for 48 h at 4°C before decalcification in 10% EDTA (pH 7.4) for approximately 4 weeks at 4°C with regular changes. Tissues were finally dehydrated and embedded in paraffin wax, using standard procedures, and sectioned at 5 µm. For immunohistochemical analysis, sections were dewaxed in xylene and rehydrated before incubation at 37°C for 30 min in 0.1% trypsin (Sigma) for antigen demasking. Endogenous peroxidases were blocked by 0.03% H2O2 in methanol (Sigma) for 30 min. Antibodies to IGFBP3 and IGF‐2 (both Santa Cruz Biotechnology, Heidelberg, Germany) were diluted to 0.4 μg IgG/ml and 0.5 μg IgG/ml, respectively and incubated for 18 h at 4°C. Control sections were incubated with an equal concentration IgG. A Vectastain Rabbit ABC kit (Vector Laboratories, Peterborough) was then used according to the manufacturer's instructions. The sections were finally dehydrated, counterstained with hematoxylin and mounted in DePeX.
Dobie R., Ahmed S.F., Staines K.A., Pass C., Jasim S., MacRae V.E, & Farquharson C. (2015). Increased linear bone growth by GH in the absence of SOCS2 is independent of IGF‐1. Journal of Cellular Physiology, 230(11), 2796-2806.
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3

Immunohistochemical Detection of GFP

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Formalin fixed tissue samples were dehydrated and embedded in paraffin. Microtome sections (4 µm) were prepared and the virally expressed GFP was detected applying a specific antibody (anti GFP, ab290, Abcam, Cambridge, UK) and the Vectastain rabbit ABC Kit (Vector Laboratories, Burlingame, CA, USA). Haematoxylin (Gill; Roth, Karlsruhe, Germany) was used for counterstaining.
Zimmermann M., Armeanu-Ebinger S., Bossow S., Lampe J., Smirnow I., Schenk A., Lange S., Weiss T.S., Neubert W., Lauer U.M, & Bitzer M. (2014). Attenuated and Protease-Profile Modified Sendai Virus Vectors as a New Tool for Virotherapy of Solid Tumors. PLoS ONE, 9(3), e90508.
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4

Immunohistochemical Detection of NTS Receptors

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All testis tissues were formalin fixed, paraffin embedded, and sectioned at 5 μm. Immunocytochemical detection of NTS receptors was performed essentially as previously described (Bender et al., 2018). Briefly, tissue sections were heated and deparaffanized. Immunodetection of NTSR1 proceeded without antigen retrieval, NTSR2 was performed after acidic antigen retrieval (Bender et al., 2018), and SORT immunodetection was performed after basic antigen retrieval (Bender et al., 2018). Slides were blocked with 5% non-immune serum in PBS containing 0.1% Triton X-100, then incubated overnight with primary antibodies against NTSR1 (Thermofisher #PA3-214; 1:400 dilution), NTSR2 (EMD Millipore #AB15134; 20 μg/ml), or SORT1 (Sigma Aldrich #HPA006889; 4 μg/ml). Omission of the primary antibody served as a negative control. Slides were incubated with DAB substrate using the Vectastain Rabbit ABC kit (Vector Laboratories, Burlingame, CA) according to manufacturer instructions. Slides were counterstained in hematoxylin, dehydrated, and permanently sealed with a coverslip. All images were obtained using an Olympus BX41 microscope fitted with a DP70 digital camera and associated software.
Martínez-Rivas J.M, & Vega J.M. (1998). Purification and Characterization of NAD-Isocitrate Dehydrogenase from Chlamydomonas reinhardtii. Plant Physiology, 118(1), 249-255.
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