All animals were fasted for 4 hr before necropsy and blood collection. The blood samples were collected from the abdominal vena cava using a syringe with a 26-gauge needle under isoflurane anesthesia (Ilsung Pharm, Seoul, Korea) and collected into vacutainers containing EDTA-3K (Becton Dickinson, Franklin Lakes, NJ, USA). The samples were analyzed within 20 min of collection using an automated hematology analyzer (Hemavet 950, Drew Scientific, Waterbury, CT, USA). The following parameters were determined in this study: total erythrocyte counts (RBC), hemoglobin concentration (HGB), hematocrit (HCT), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC), platelet count (PLT), whole leukocyte counts (WBC), neutrophils (NEU, %), eosinophils (EOS, %), basophils (BASO, %), lymphocytes (LYM, %) and monocytes (MONO, %).
Edta 3k
Manufactured by BD
Sourced in United States
EDTA-3K is a laboratory reagent used primarily as an anticoagulant in the collection and preparation of blood samples for analysis. It functions by chelating calcium ions, preventing blood coagulation.
Automatically generated - may contain errors
5 protocols using edta 3k
1
Hematological Profiles in Animal Study
2
Hematological Analysis of Fasted Rats
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Five male and five female rats in each group were fasted overnight before the necropsy and blood collection. Blood samples were taken from the abdominal aorta using a syringe with a 21-gauge needle under isoflurane anesthesia (Ilsung Pharm, Seoul, Korea) and collected into vacutainers containing EDTA-3K (Becton Dickinson, Franklin Lakes, NJ, USA). The samples were analyzed within 20 min of collection using an automated hematology analyzer (F-820, Sysmex, Kobe, Japan) The following parameters were determined in this study: total erythrocyte counts, hemoglobin concentration, hematocrit, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration, platelet count, and whole leukocyte counts.
3
Comprehensive Blood Analysis Protocol
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All animals were fasted overnight before necropsy and blood collection. Blood samples were taken from the abdominal aorta using a syringe with a 24-gauge needle under Isoflurane anesthesia (Ilsung Pharm, Seoul, Korea) and were collected into vacutainers containing EDTA-3K (Becton Dickinson, Franklin Lakes, NJ, USA). Samples were analyzed within 20 min of collection using an automated hematology analyzer (Hemavet 950, Drew Scientific, Waterbury, CT, USA) and an automated coagulation analyzer (HumaClot Duo, Human, Wiesbaden, Germany) with plasma obtained after blood was mixed with 3.2% sodium citrate (9NC sodium citrate, Becton Dickinson). The following parameters were determined in this study: total erythrocyte counts (RBC), hemoglobin concentration (HGB), hematocrit (HCT), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC), red cell distribution (RDW), platelet count (PLT), mean platelet volume (MPV), prothrombin time (PT) and activated partial thromboplastin time (APTT), whole leukocyte counts (WBC), neutrophils (NEU, %), eosinophils (EOS, %), basophils (BASO, %), lymphocytes (LYM, %) and monocytes (MONO, %).
4
Comprehensive Hematological Analysis
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Each blood sample was transferred to CBC bottles (EDTA 3 K; BD Biosciences, San Jose, CA, USA) and assayed on a hematology analyzer (Advia 120E; Siemens Healthcare, Malvern, PA USA). Hematological measurements included eosinophil (EOS), mean corpuscular hemoglobin concentration (MCHC), hematocrit (HCT), neutrophil (NEU), hemoglobin (HGB), mean corpuscular volume (MCV), large unstained cell (LUC), lymphocyte (LYM), red blood cell (RBC), white blood cell (WBC), platelet (PLT), mean corpuscular hemoglobin (MCH), monocyte (MONO), mean platelet volume (MPV), basophil (BASO), red cell distribution width (RDW), and hemoglobin distribution width (HDW).
5
Hematological Analysis in Fasted Rats
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The rats were fasted overnight before necropsy. Blood was collected from the abdominal aorta under anesthesia with isoflurane. Three milliliters of blood was collected in a CBC bottle (EDTA 3 K; BD Biosciences, San Jose, CA, USA) and analyzed using an autohematoanalyzer (Advia120E; Siemens). The remaining blood was transferred into Vacutainer® tubes (sodium citrate 3.2%, BD Biosciences) and centrifuged at 3,000 rpm for 10 minutes. The plasma was separated and clotting time was determined using a coagulometer (ACL 7000; Instrumentation Laboratory, Bedford, MA, USA). The hematological parameters studied were as follows: total leukocyte and differential leukocyte (neutrophil, lymphocyte, monocyte, eosinophil, and basophil) count, total erythrocyte count, hemoglobin concentration, hematocrit, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration, reticulocytes, platelets, prothrombin time, and activated partial thromboplastin time.
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