The fasting vein blood was taken from the cubital vein of all subjects and centrifuged at 3000 rpm for 10 mins at 4°C straightaway. Then, the serum was aliquoted and stored at −80°C until analysis. Levels of endostatin, C-reactive protein (CRP) and VEGF were detected using Human Magnetic Luminex Screening Assay (LXSAHM; R&D Systems, Inc. Minneapolis, MN, USA) on Bio-Plex 200 detection platform (Bio-Rad, California, USA) according to the manufacturer’s instructions. The lower limits of detection were 27.3 pg/mL for endostatin, 116pg/mL for CRP and 2.1pg/mL for VEGF. Operators performing measurements did not know the clinical information of the subjects.
Bio plex 200 detection platform
Manufactured by Bio-Rad
Sourced in United States, China
The Bio-Plex 200 is a detection platform designed for multiplex assays. It utilizes fluorescent-coded magnetic beads and laser-based detection to enable the simultaneous measurement of multiple analytes in a single sample.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using bio plex 200 detection platform
1
Biomarker Levels in Fasting Blood
2
Serum Biomarkers in Clinical Assessment
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Venous blood was collected from all subjects and immediately centrifuged at 3000 rpm for 10 mins at 4°C. The serum samples were subsequently stored at −80°C until analysis. Levels of SDC-1, SDC-4, and C-reactive protein (CRP) were analyzed using Human Magnetic Luminex Screening Assay (LXSAHM; R&D Systems China Co., Ltd. Shanghai, China) on Bio-Plex 200 detection platform (Bio-Rad, Hercules, CA, USA) by the Department of Laboratory Medicine of West China Hospital strictly according to the manufacturer’s instructions. Technicians performing tests were blinded to the clinical details of the subjects. Blood samples were detected by an SF-3000 blood counter system, and white blood cells were categorized.
3
Serum Biomarkers of Cardiometabolic Health
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Subjects were asked to fast overnight from 21:00 the night before, after which venous blood samples were collected and serum was separated immediately and stored at −80 °C until analysis. Levels of CTRP-3 and CTRP-5 were analyzed using an enzyme-linked immunosorbent assay (ELISA; AdipoGen, Incheon, Korea). Adiponectin, CRP, TNF-α, and MPO were analyzed using Human Magnetic Luminex Screening Assay (LXSAHM; R&D Systems China Co., Ltd Shanghai, China) on the Bio-Plex 200 detection platform (Bio-Rad, CA) by the Department of Laboratory Medicine of West China Hospital. All the measurements were carried out strictly according to the manufacturer's instructions. The detection sensitivities were 1 ng/ml for CTRP-3 and CTRP-5, 148 pg/ml for adiponectin, 116 pg/ml for CRP, 26.2 pg/ml for MPO, and 1.2 pg/ml for TNF-α. Technicians performing tests were blinded to the clinical details of the subjects.
4
Quantification of Interferon Levels
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The plasma of all participants, previously isolated and stored at -80°C, was used to measure the concentration of IFN-beta (IFN-β) and IFN-gamma (IFN-γ) Human Magnetic Luminex Screening Assays (LXSAHM; R&D Systems, Inc. Minneapolis, MN, United States), according to the manufacturer’s instructions. Duplicates measurements were performed per sample and a standard curve was created on each plate. Microparticle cocktail and biotin-antibody cocktail were added to each individual sample or standard and co-incubated on a shaker overnight (800 rpm, 4°C). Samples were washed and incubated with streptavidin-PE for 30 min (800 rpm, 20°C). After washing, the samples were resuspended in 100 μL wash buffer and analyzed with the software on Bio-Plex 200 detection platform (Bio-Rad, California, United States). The concentration of each analyte was measured in relation to the individual calibration curve and the results were expressed in pg/ml.
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