The reagents and solvents were purchased from commercial sources (Fisher) and used directly. The intermediates and products were purified by using an ISCO CombiFlash system and prepacked SiO2 cartridges. Final compounds were purified on preparative high-pressure liquid chromatography (RP-HPLC) was performed on Agilent 1260 Series system. Systems were run with 0–20% methanol/water gradient with 0.1% TFA. NMR spectra were acquired on a Bruker AV500 instrument (500 MHz for 1H-NMR, 126 MHz for 13C-NMR). TLC-MS were acquired using Advion CMS-L MS. Matrix-assisted laser desorption ionization mass spectra (MALDI-MS) data were acquired in positive-ion mode using a Sciex 4800 MALDI TOF/TOF MS. The peptides were synthesized using a CEM Liberty Blue Automated Microwave Peptide Synthesizer with the manufacturers standard coupling cycles at 0.1 mmol scale. The purity of final compounds was confirmed by Waters LC-MS system and/or Agilent 1260 Series HPLC system. Systems were run with 0–40% methanol/water gradient with 0.1% TFA. All the purity of target compounds showed >95% in RP-HPLC.
Av500 instrument
Manufactured by Bruker
Sourced in United States
The AV500 instrument is a high-performance Nuclear Magnetic Resonance (NMR) spectrometer designed for analytical and research applications. It provides a stable magnetic field and advanced radio frequency (RF) capabilities to enable the acquisition and analysis of NMR data.
Automatically generated - may contain errors
Lab products found in correlation
Cms l ms, by Advion (3 mentions)
Agilent 1260 series system, by Agilent Technologies (3 mentions)
Combiflash purification system, by Teledyne (2 mentions)
Agilent 1260 infinity 2 variable wavelength detector, by Agilent Technologies (2 mentions)
Zorbax rr sb c18, by Agilent Technologies (2 mentions)
Beh c18, by Waters Corporation (2 mentions)
Lc ms system, by Agilent Technologies (1 mentions)
Agilent 1260 series hplc system, by Agilent Technologies (1 mentions)
4800 maldi tof tof ms, by AB Sciex (1 mentions)
Combiflash system, by Teledyne (1 mentions)
Av 400 instrument, by Bruker (1 mentions)
Microqtof spectrometer, by Bruker (1 mentions)
Melting point m 560, by Büchi (1 mentions)
Uv 1800 spectrophotometer, by Shimadzu (1 mentions)
Ssx 550, by Shimadzu (1 mentions)
Zetasizer 3000hsa, by Malvern Panalytical (1 mentions)
Water q tof micro mass spectrometer, by Waters Corporation (1 mentions)
Mitotracker, by Beyotime (1 mentions)
6 protocols using av500 instrument
1
Automated Synthesis of Peptides
2
Synthesis and Characterization of (R3P)AuCl Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reactions were performed under an atmosphere of argon by using standard Schlenk or dry box techniques; solvents were dried over Na metal or CaH2 under nitrogen atmosphere. (R3P)AuCl (R = Me, Et) were synthesized using literature procedures66 (link)–68 (link). 1H, 13C, 29Si, and 31P NMR spectra were obtained with a Bruker AV 400 instrument at 400 MHz (1H NMR), 101 MHz (13C NMR) and 162 MHz (31P NMR), as well as Bruker AV 500 instrument at 500 MHz (1H NMR), 126 MHz (13C NMR), 99 MHz (29Si NMR), 202 MHz (31P NMR) at 298 K. Unless otherwise noted, the NMR spectra were recorded in benzene-d6 at ambient temperature. The 1H and 13C NMR chemical shifts were referenced to residual 1H and 13C signals of the solvents. NMR multiplicities are abbreviated as follows: s = singlet, d = doublet, t = triplet, dt = doublet of triplets, m = multiplet, and brs = broad singlet. Coupling constants J are given in Hz. Electrospray ionization (ESI) mass spectra were obtained at the Mass Spectrometry Laboratory at Hangzhou Normal University with a Bruker Daltonics MicroQtof spectrometer. Melting points were measured with a BUCHI Melting Point M-560. Sampling of air-sensitive compounds was carried out using a MBRAUN’s MB-10-G glove box. UV–Vis spectra were recorded on a Shimadzu UV-1800 spectrophotometer.
3
Synthesis and Characterization of Phosphorus-based Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents and solvents were commercially available and used without further purification, unless otherwise noted. Compound (P 5 A ) (Ogoshi et al., 2008 (link); Cao et al., 2009 (link)) and bis(pyridinium)dicationic 1 (Joseph et al., 2003 (link); Li et al., 2010 (link)) were synthesized according to literature procedures. 1H NMR and DOSY spectra were recorded on a Bruker AV500 instrument. Viscosity measurements were carried out with Ubbelohde micro dilution viscometers (Shanghai Liangjing Glass Instrument Factory, 0.40 mm inner diameter) at 298 K in DMSO. Dynamic light scattering (DLS) was analyzed on a Malvern Zetasizer 3000HSA at 298 K. Scanning electron microscopy (SEM) images were recorded on SHIMADZU SSX-550.
4
Metabolic Profiling of Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal procedures were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of Jinan University, and experiments were approved by the Animal Ethics Committee of Jinan University. Mito-Tracker was purchased from Beyotime Biotechnology (Shanghai, China). All reagents were purchased from commercial suppliers. ESI-mass and high-resolution mass spectra (HRMS) were performed on a Water QTOF micro mass spectrometer (Waters, Milford, USA). Bruker AV-500 instrument (Billerica, USA) was used to record 1H NMR and 13C NMR.
5
Purification and Characterization of Novel Compounds
6
Purification and Characterization of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The reagents and solvents were purchased from commercial sources (Fisher and Sigma-Aldrich) and used directly. Analytical thin-layer chromatography was performed on ready-to-use plates with silica gel 60 (Merck, F254). Flash column chromatography was performed over silica gel (grade 60, 230-400 mesh) on Teledyne Isco CombiFlash purification system. Final compounds were purified on preparative reversed phase high-pressure liquid chromatography (RP-HPLC) and was performed on Agilent 1260 Series system with Agilent 1260 Infinity II Variable Wavelength Detector (G7114A, UV = 254 nM) and a Waters BEH C18 (130Å, 5 µm, 10 mm X 250 mm) column. Final compounds were eluted using a gradient 95% water (with 0.1% formic acid) and 5% acetonitrile (with 0.1% formic acid) at a flow rate of 4 mL/min over 30 min.
NMR spectra were acquired on a Bruker AV500 instrument (500 MHz for 1 H-NMR, 126 MHz for 13 C-NMR). TLC-MS were acquired using Advion CMS-L MS. The Agilent 1260 Infinity II Variable Wavelength Detector (G7114A, UV = 254 nM) and an Agilent ZORBAX RR SB-C18 (80Å, 3.5 µm, 4.6 x 150 mm) at a flow rate of 1 mL/min using a solvent system of 100% water with 0.1% TFA to 40 or 60% methanol over 20min were used to assess purity of final compounds.
All the purity of target compounds showed >95% in RP-HPLC. FP was monitored on a BMG CLARIOstar microplate reader.
NMR spectra were acquired on a Bruker AV500 instrument (500 MHz for 1 H-NMR, 126 MHz for 13 C-NMR). TLC-MS were acquired using Advion CMS-L MS. The Agilent 1260 Infinity II Variable Wavelength Detector (G7114A, UV = 254 nM) and an Agilent ZORBAX RR SB-C18 (80Å, 3.5 µm, 4.6 x 150 mm) at a flow rate of 1 mL/min using a solvent system of 100% water with 0.1% TFA to 40 or 60% methanol over 20min were used to assess purity of final compounds.
All the purity of target compounds showed >95% in RP-HPLC. FP was monitored on a BMG CLARIOstar microplate reader.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!