Foxp3dtr

Manufactured by Jackson ImmunoResearch

Sourced in Montenegro

Foxp3DTR is a lab equipment product that is used for the detection and analysis of Foxp3-expressing regulatory T cells. The product utilizes a diphtheria toxin receptor (DTR) system to specifically target and deplete Foxp3-positive cells, enabling the study of their role in various biological processes.

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Lab products found in correlation

21 protocols using foxp3dtr

Genetic Mouse Models for Immune Cell Research

Cited 4 times

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Ppp3r1^f/f (here referred to as CnB^f/f) (Neilson et al., 2004 (link)), Foxp3^cre (Rubtsov et al., 2008 (link)), Foxp3^creERT2 (Rubtsov et al., 2010 (link)), Foxp3^GFP (Lin et al., 2007 (link)), Foxp3^DTR (Kim et al., 2007 (link)), zDC^DTR (Zbtb46^DTR) (Meredith et al., 2012 (link)), C57BL/6, C57BL/6 CD45.1, and BALB/c mice were from Jackson laboratories. Cd28^f/f (Zhang et al., 2013 (link)), Cd11c^mCherry (Khanna et al., 2010 (link)), Ctla4^f/f (Paterson et al., 2015 (link)), Il2^GFP (DiToro et al., 2018 (link)), and pgk-HA × TCR-HA transgenic mice as a source of HA-specific Treg cells (Klein et al., 2003 (link)) were directly obtained from the investigators who had generated them. Mice were enrolled in experiments at 6–12 weeks of age. Per NIH guidelines, both male and female mice were used for experiments whenever possible, and results pooled when comparable. In all cases, mice were bred, housed, enrolled in experiments and euthanized according to protocols approved by the Institutional Animal Care and Use Committee (IACUC) of the Massachusetts General Hospital and of the University of California - Irvine.

Marangoni F., Zhakyp A., Corsini M., Geels S.N., Carrizosa E., Thelen M., Mani V., Prüßmann J.N., Warner R.D., Ozga A.J., Di Pilato M., Othy S, & Mempel T.R. (2021). Expansion of tumor-associated Treg cells upon disruption of a CTLA-4-dependent feedback loop. Cell, 184(15), 3998-4015.e19.

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Generation of Transgenic Mice for Immunology Research

Cited 3 times

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All mice used in this study were produced in our specific pathogen–free facilities at Harvard Medical School (HMS), and the experiments were conducted under protocols approved by HMS’s Institutional Animal Care and Use Committee. The following strains on B6 background were used: Foxp3-IRES-GFP mice were obtained from V. Kuchroo (Brigham and Women’s Hospital, Boston, MA); the Kaede transgenic (tg) line (Kaede/B6) was obtained from O. Kanagawa (RIKEN, Wako, Japan; Tomura et al., 2008 (link)) and were crossed in-house to Foxp3^Thy1.1 knock-in mice (from A. Rudensky, Memorial Sloan Kettering Cancer Center, New York, NY); Nr4a1^EGFP (Nur77^GFP) transgenic mice (Moran et al., 2011 (link)) were purchased from The Jackson Laboratory (#016671) and were crossed to Foxp3^Thy1.1 mice; ST2-deficient mice (Il1rl1^tm1Anjm; Townsend et al., 2000 (link)) were obtained from A. McKenzie and R. Lee (Brigham and Women’s Hospital, Boston, MA); and Foxp3-IRES-GFP-hDTR (Foxp3^DTR) mice were obtained from A. Rudensky. All mice, except those expressing Foxp3^DTR, were further bred with B6.Aire^+/− mice to generate F1 progenies that served as parents for the generation of Aire^+/+ and Aire^−/− perinates. NOD.Aire^−/− and Aire^+/+ mice (Jiang et al., 2005 (link)) were from our own colony at The Jackson Laboratory. All experiments were performed using sex-matched littermate controls.

Tuncel J., Benoist C, & Mathis D. (2019). T cell anergy in perinatal mice is promoted by T reg cells and prevented by IL-33. The Journal of Experimental Medicine, 216(6), 1328-1344.

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Genetically Modified Mouse Models

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Male (6wk) C57BL/6 mice were purchased from Taconic Biosciences (Hudson, NY). 4–1BB^−/−, EBI3^−/−, IL27 receptor alpha^−/−, β2M^−/−, MHCII^−/−, Foxp3-DTR, CXCR3^−/−, CCR2^−/−, and CCR5^−/− mice were purchased from the Jackson Laboratory (Bar Harbor, ME). All procedures were conducted in accordance with the guidelines established by the U.T. MD Anderson Cancer Center Institutional Animal Care and Use Committee.

Bartkowiak T., Jaiswal A.R., Ager C.R., Chin R., Chen C.H., Budhani P., Ai M., Reilley M.J., Sebastian M.M., Hong D.S, & Curran M.A. (2018). Activation of 4-1BB on liver myeloid cells triggers hepatitis via an interleukin-27 dependent pathway. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(5), 1138-1151.

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Genetically Modified Mice for Immune Research

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All animals were maintained in specific pathogen-free barrier facilities and used in accordance with protocols approved by the Institutional Animal Care and User Committee at the Institute of Hematology, Chinese Academy of Medical Sciences. C57BL/6, Lkb1^f/f, AMPKα1^f/f, AMPKα2^f/f, Cd11c^Cre, Foxp3^YFP-Cre, LysM^Cre, Foxp3^DTR and NOD/scid IL2Rg^null (NSG) mice were purchased from Jackson Laboratories. All mice had been backcrossed with C57BL/6 mice for at least seven generations. Lkb1^f/f, AMPKα1^f/f and AMPKα2^f/f mice were crossed with Cd11c^Cre, LysM^Cre to generate Cd11c^CreLkb1^f/f, Cd11c^CreAMPKα1^f/fAMPKα2^f/f, and LysM^CreLkb1^f/f mice, respectively. Cd11c^CreLkb1^f/f mice were crossed with Foxp3^DTR mice to generate Cd11c^CreLkb1^f/fFoxp3^DTR mice. All mice were used when 6–8 weeks old unless otherwise noted. The sample size was selected to maximize the chance of uncovering a mean difference with statistical significance. No statistical methods were used to predetermine the sample size. The experiments were not randomized, and the investigators were not blinded to group allocation during the experiments or outcome assessments.

Chen S., Fang L., Guo W., Zhou Y., Yu G., Li W., Dong K., Liu J., Luo Y., Wang B., Li Z., Zhao C., Sun Z., Shen Y., Leng Q., Zhou D., Han Z., Huang H., Ren H., Xu G, & Feng X. (2018). Control of Treg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells. Nature Communications, 9, 5298.

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Mice Genotypes and Sourcing

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Six week-old female Flt3LKO mice were purchased from Taconic. C57BL/6, CD11c-DTR, CD11c-Cre, Foxp3-DTR and Pten-flox mice were purchased from the Jackson Laboratory. Flk2KO mice were kindly provided by Ihor Lemischka from Ichan School of Medicine at Mount Sinai. Batf3KO mice were gifts from Kenneth Murphy from Washington University. DC-specific Pten knockout mice (DC^ΔPTEN) were generated by breeding CD11c-Cre with Pten-flox mice. Cre-negative littermates (Pten-Flox) were used as control. All procedures were in accordance with Institutional Animal Care and Use Committee Protocols.

Jiao J., Dragomir A.C., Kocabayoglu P., Rahman A.H., Chow A., Hashimoto D., Leboeuf M., Kraus T., Moran T., Carrasco-Avino G., Friedman S.L., Merad M, & Aloman C. (2014). Central Role of Conventional Dendritic Cells in Regulation of Bone Marrow Release and Survival of Neutrophils. Journal of immunology (Baltimore, Md. : 1950), 192(7), 3374-3382.

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Genetically Modified Mouse Models for Neuroimmunology

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C57BL/6J, Spp1^−/−, Rag1^−/−, Foxp3^DTR, Itgb1^flox/flox, and Cx3cr1^CreER mice were purchased from the Jackson Laboratory. Cx3cr1^CreER+/−Itgb1^f/f mice were bred from Itgb1^flox/flox and Cx3cr1^CreER(+/−) mice. The depletion of Itgb1 in microglia and macrophages was induced in Cx3cr1^CreER+/−Itgb1^f/f mice (9 week-old males) by intraperitoneal injection of 4-hydroxytamoxifen (0.1 mg in 100 μL corn oil, daily for 5 consecutive days). Male mice (8-12 week-old) were used for in vivo experiments. All animal procedures were approved by the University of Pittsburgh Institutional Animal Care and Use Committee (approval number: 18032546), performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals, and reported in accordance with the Animal Research: Reporting In Vivo Experiments (ARRIVE) guidelines (Percie du Sert et al., 2020 ). All efforts were made to minimize animal suffering and the number of animals used. All animals were housed in a temperature and humidity-controlled facility with a 12-h light/dark cycle. Food and water were available ad libitum. Animals were randomly assigned to sham or stroke groups and received randomized treatments using a lottery drawing box. All treatments and analyses were performed by blinded investigators wherever feasible.

Shi L., Sun Z., Su W., Xu F., Xie D., Zhang Q., Dai X., Iyer K., Hitchens T.K., Foley L.M., Li S., Stolz D.B., Chen K., Ding Y., Thomson A.W., Leak R.K., Chen J, & Hu X. (2021). Treg cell-derived osteopontin promotes microglia-mediated white matter repair after ischemic stroke. Immunity, 54(7), 1527-1542.e8.

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Genetic Lineage Tracing in Pancreatic Cancer Mice

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The conditional LSL-Kras^G12D mice and p48-Cre mice were previously described (Pylayeva-Gupta et al., 2012 (link)). To perform lineage tracing, a Rosa^tdTomato reporter allele was introduced into p48-Cre and p48-Cre;Kras^G12D strains of mice to generate p48-Cre;Rosa^tdTomato (CT) and p48-Cre;Kras^G12D;Rosa^tdTomato (KCT), respectively. Foxp3-EGFP (Bettelli et al., 2006 (link)), CD11c-EYFP (Lindquist et al., 2004 (link)), Foxp3^DTR (Kim et al., 2007 (link)), and CD8a-Cre, and Rosa^tdTomto mice were purchased from The Jackson Laboratory and C57BL/6 from National Cancer Institute (NCI) or Charles River Laboratories. Foxp3-EGFP or CD8a-Cre;Rosa^tdtomato female mice were crossed with CD11c-EYFP male mice to generate Foxp3-EGFP;CD11c-EYFP or CD8a-Cre;Rosa^tdtomato;CD11c-EYFP mice. All mice were on a C57BL/6 genetic background. The Institutional Animal Care and Use Committee at the New York University (NYU) School of Medicine approved all animal care and procedures.

Jang J.E., Hajdu C.H., Liot C., Miller G., Dustin M.L, & Bar-Sagi D. (2017). Crosstalk between Regulatory T Cells and Tumor-Associated Dendritic Cells Negates Anti-tumor Immunity in Pancreatic Cancer. Cell Reports, 20(3), 558-571.

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Genetically Modified Mouse Models

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6–10-wk-old C57BL/6 CD45.2 and CD45.1 mice were purchased from the US National Cancer Institute. Cdkn1a^−/−, Nfe2l2^−/−, Atm^−/−, Ccr7^−/−, Foxp3-DTR, MOG-specific 2D2 transgenic, and H2^–/–mice were purchased from the Jackson Laboratory. Foxo3^−/− and Trp53^−/− mice were obtained from S. Ghaffari. Ly75^−/− mice were obtained from M.C. Nussenzweig. All animal protocols were approved by the Icahn School of Medicine IACUC.

Price J.G., Idoyaga J., Salmon H., Hogstad B., Bigarella C.L., Ghaffari S., Leboeuf M, & Merad M. (2015). The cell cycle inhibitor Cdkn1a regulates Langerhans cell radiation resistance and promotes T regulatory cell generation upon exposure to ionizing irradiation. Nature immunology, 16(10), 1060-1068.

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Murine Lung Metastasis Model

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Six- to twelve-week-old B6129SF1/J (C57BL/6J × 129S1/SvImJ F1) mice were used as hosts in the LMP model unless otherwise indicated, and obtained from The Jackson Laboratory or bred in-house. C57BL/6J, Csf2rb KO, Foxp3^DTR, and B6.SJL-Ptprc^aPepc^b/BoyJ (CD45.1) mice were obtained from Jackson and used for generating BM chimeras or crossed with 129S1/SvImJ mice to obtain tumor hosts. Mgl2^DTR mice on a C57BL/6 background (39 (link)) were crossed with 129S1/SvImJ mice to obtain tumor hosts. All procedures were approved by the Institutional Animal Care and Use Committee of Stanford University.

Kenkel J.A., Tseng W.W., Davidson M.G., Tolentino L.L., Choi O., Bhattacharya N., Seeley E.S., Winer D.A., Reticker-Flynn N.E, & Engleman E.G. (2017). An immunosuppressive dendritic cell subset accumulates at secondary sites and promotes metastasis in pancreatic cancer. Cancer research, 77(15), 4158-4170.

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Genetically Modified Mice for Immunology

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C57BL/6, Rag1^−/−, μMT^−/−, β₂M^−/−, C-II^−/− (H2^dlAb1-Ea), Il17a^−/−, Tbx21^−/−, and Foxp3^DTR mice were purchased from the Jackson Laboratory. Il22^−/− mice were provided by R. Flavell (Yale University). All mouse strains were derived on a C57BL/6 background. All mice were bred and maintained in sterile autoclaved cages under specific pathogen-free conditions and kept on a grain-based diet (Labdiet 5053) at the Memorial Sloan Kettering Research Animal Resource Center or the University of Pennsylvania. Mice were provided autoclaved water ad libitum from water bottles. Sex and age-matched controls were used in all experiments according to institutional guidelines for animal care. All animal procedures were approved by the Institutional Animal Care and Use Committee of the Memorial Sloan Kettering Cancer Center and University of Pennsylvania.

Littmann E.R., Lee J.J., Denny J.E., Alam Z., Maslanka J.R., Zarin I., Matsuda R., Carter R.A., Susac B., Saffern M.S., Fett B., Mattei L.M., Bittinger K, & Abt M.C. (2021). Host immunity modulates the efficacy of microbiota transplantation for treatment of Clostridioides difficile infection. Nature Communications, 12, 755.

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