Hc pl fluotar 10 0.30 objective

For competition experiments, printed arrays in LB-Miller media were incubated at 37 °C for 18 h. After 18 h, printed arrays were washed with M9 medium to remove excess bacteria that grew out into solution from the printed arrays during the competition experiment. Within each compartment containing a printed array, 400 μL of LB-Miller medium were removed and 200 μL of M9 medium were added. Following this, 200 μL of the medium were removed and another 200 μL of M9 medium were added to further wash and remove the bacteria. Finally, printed arrays were picked up using a cut pipette tip (20 μL) and transferred into individual containers containing 400 μL of M9 media (Nunc^TM, Lab-Tek^TM II Chambered Coverglass (eight wells)). Printed arrays were left in M9 media for 10 h to allow the exchange of LB medium with M9 medium, required for imaging. Printed arrays were imaged using a Leica^® SP5 confocal microscope using a HC PL Fluotar ×10/0.30 objective (0.3 numerical aperture), at excitation wavelengths of 405 nm and 546 nm and emission cut-offs at 420–520 nm and 625 nm, respectively (exciting superfolder GFP, mRFP1, YPet and mNeonGreen), a z-step of 2.73 µm and scanning speed of 400 Hz.