Protein samples were diluted in Nu-PAGE lithium dodecyl sulfate (LDS) sample buffer (Invitrogen) and heated for 30 minutes at 90 °C. 15 μL of protein samples and 2 μL of PageRuler Plus Prestained Protein Ladder were pipetted into the well of a 4–12% Bi-Tris gel (Invitrogen). Gels were then run in MES-SDS buffer at 4 °C for 60 minutes at 110 V. Afterwards, the gel was washed in DI water and stained with Imperial Protein Stain for 30 minutes then destained overnight. Gels were then imaged with ChemiDoc MP imaging system and Image Lab 5.2.1 software (Bio-Rad).
Bi tris gel
Manufactured by Thermo Fisher Scientific
The Bi-Tris gel is a type of polyacrylamide gel used for protein electrophoresis. It is designed to provide high-resolution separation of proteins based on their molecular weight. The gel utilizes a Bis-Tris buffer system, which maintains a neutral pH during the electrophoresis process.
Automatically generated - may contain errors
2 protocols using bi tris gel
1
SDS-PAGE Protein Separation and Visualization
2
SDS-PAGE Protein Sample Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein samples were diluted in Nu-PAGE lithium dodecyl sulfate (LDS) sample buffer (Invitrogen) and heated for 30 min at 90 °C. 15 μL of protein samples and 2 μL of PageRuler Plus Prestained Protein Ladder were pipetted into the well of a 4–12% Bi-Tris gel (Invitrogen). Gels were then run in MES-SDS buffer at 4 °C for 60 min at 110 V. Afterwards, the gel was washed in DI water and stained with Imperial Protein Stain for 30 min then destained overnight. Gels were then imaged with ChemiDoc MP imaging system and Image Lab 5.2.1 software (Bio-Rad).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!