N hexane

ultrapure LPS from E. coli K12 (LPS-EC), ultrapure LPS from Rhodobacter sphaeroides (LPS-RS), PAM3CSK4 (PAM), HEK-Blue selection antibiotics, Normocin, Zeocin and QUANTI-Blue were all purchased from InvivoGen Europe (Toulouse, France). The α-TLR2 blocking antibody was obtained from R&D systems. Recombinant human GM-CSF was purchased from PeproTech (Rocky Hill, NJ, USA) and recombinant human IL-4 was purchased from Sanquin (Amsterdam, The Netherlands). The IL-6 ELISA kit was purchased from Sanquin, the IL-12p40 ELISA kit was purchased from Diaclone and the IL-8 ELISA kit was obtained from R&D systems. Dulbecco’s modified Eagle’s medium (DMEM) and Iscove’s modified Dulbecco’s medium (IMDM) were purchased from Gibco, FCS was obtained from Thermo scientific (Waltham, MA). Fatty acid standards C14:0, C14:0-3OH, NaOH, HCl and tert-butyl methyl ether were purchased from Sigma (Zwijndrecht, The Netherlands). Methanol and n-Hexane were from JT Baker and n-Hexane from Biosolve.

Polyethylene glycol (PEG, M_w ∼ 1500 Da) was supplied by Fluka Chemie
GmbH (Buchs, Switzerland). Polypropylene glycol (PPG, M_w ∼ 1010 Da, M_w/M_n 1.04) was purchased from PSS Polymer Standards
Service GmbH (Mainz, Germany). Polytetrahydrofuran of M_w ∼ 1000 Da and M_w ∼
1400 Da (denoted as PTHF1000 and PTHF1400, respectively) was obtained
from Royal DSM N.V. (Heerlen, The Netherlands). Nylon-6 was purchased
from Scientific Polymer Products Inc. (M_w ∼ 25,000 Da, Oregon, USA). Polystyrene (PS, M_w ∼ 1300 Da, M_w/M_n 1.10) was purchased from Thermo Fisher Scientific
GmbH (Kandel, Germany). Polybutylene terephthalate (PBT, M_w ∼ 16,150 Da). ULC-MS-grade methanol (MeOH), ethanol
(EtOH), and n-hexane were acquired from Biosolve
B.V. (Valkenswaard, The Netherlands). Tetrahydrofuran (THF) and hexafluoroisopropanol
(HFIP, ≥99% purity) were supplied by Sigma-Aldrich Chemie B.V.
(Zwijndrecht, The Netherlands). All organic solvents
were used without further purification. 2,5-Dihydroxybenzoic acid
(DHB, 98% purity), dithranol (DT, ≥90% purity), 2′,4′,6′-trihydroxyacetophenone
monohydrate (THAP, ≥99.5% purity), silver trifluoroacetate
salt (AgTFA, 98% purity), and trifluoroacetic acid (TFA, ≥99%
purity) were purchased from Sigma-Aldrich.

Standard antioxidants (gallic acid, quercetin, catechin, and BHT), 95° ethanol, 95° methanol, the Folin–Ciocalteu reagent, sodium carbonate (Na₂CO₃), 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH), aluminum chloride (AlCl₃), sodium acetate, vanillin, 35% hydrochloric acid (HCl), 99% Acetic acid, phosphate buffer, potassium hexacyanoferrate (K₃Fe(CN)₆), trichloroAcetic acid (TCA), ferric trichloride (FeCl₃), ascorbic acid, chloroform, and anhydrous sodium were obtained from Sigma‐Aldrich. Standard fatty acid methyl esters (C₄–C₂₂) and boron trifluoride‐methanol (14% BF₃/CH₃OH, v/v) were purchased from Sigma‐Aldrich. Internal standard of the colon (C17:0) was purchased from Sigma‐Aldrich. n‐Hexane was from Biosolve Chimie SARL. Acetic acid, HPLC grade methanol, and acetonitrile were obtained from Carlo Erba Reagents. Formic acid and Sodium fluoride were purchased from VWR Prolabo. Standards of epicatechin, hyperoside, and ideain were purchased from Extrasynthese. All the chemicals were of analytical grade.