Hepcidin

Manufactured by R&D Systems

Sourced in China

Hepcidin is a 25-amino acid peptide hormone produced primarily by hepatocytes. It plays a central role in iron homeostasis by regulating iron absorption, distribution, and storage.

Automatically generated - may contain errors

Lab products found in correlation

C reactive protein (crp), by R&D Systems (2 mentions) Adiponectin, by R&D Systems (1 mentions) Interleukin 6 (il 6), by R&D Systems (1 mentions) Leptin, by R&D Systems (1 mentions) Norepinephrine, by Abnova (1 mentions) Tnf α, by R&D Systems (1 mentions) Plasma il 6, by R&D Systems (1 mentions) Transferrin, by Thermo Fisher Scientific (1 mentions) Ferritin, by Abcam (1 mentions) 6 6 2h2 glucose, by Cambridge Isotopes (1 mentions) Il 6 levels, by R&D Systems (1 mentions) Cobas c702, by Roche (1 mentions)

Spelling variants of this product

Quantikine ELISA Human Hepcidin Immunoassay (2 citations) Human Hepcidin Quantikine ELISA kit (2 citations)

7 protocols using hepcidin

Biomarker Profiling for Metabolic Disorders

Check if the same lab product or an alternative is used in the 5 most similar protocols

IL-6, hepcidin, adiponectin, leptin, and fibroblast growth factor-21 (FGF-21) were analyzed using the respective ELISA kits: IL-6 (DY206-05; R&D Systems, Minneapolis, MN), hepcidin (DY8307-05; R&D Systems, Minneapolis, MN), adiponectin (DY1065-05; R&D Systems, Minneapolis, MN), leptin (DY398-05; R & D Systems, Minneapolis, MN), and e FGF21 (DF2100; R & D Systems, Minneapolis, MN), according to the manufacturer's instruction.

Pino J.M., Silva V.F., Mônico-Neto M., Seva D.C., Kato M.Y., Alves J.N., Pereira G.C., Antunes H.K., Galvao T.D., Bitterncourt L.R., Tufik S., Zambrano L.I., Dâmaso A.R., Oyama L.M., Thivel D., Campos R.M, & Lee K.S. (2023). Severe Obesity in Women Can Lead to Worse Memory Function and Iron Dyshomeostasis Compared to Lower Grade Obesity. International Journal of Endocrinology, 2023, 7625720.

+ Open protocol

+ Expand

Quantification of Iron Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ferroportin (BlueGene Biotech) and IREB2 (Aviva) from cell lysates and hepcidin (R&D Systems) from cell culture supernatants were quantified by ELISA according to the respective detailed manufacturer’s instructions. Ferroportin and IREB2 levels were normalised to total protein in the cell lysates.

Ramakrishnan L., Pedersen S.L., Toe Q.K., West L.E., Mumby S., Casbolt H., Issitt T., Garfield B., Lawrie A., Wort S.J, & Quinlan G.J. (2018). The Hepcidin/Ferroportin axis modulates proliferation of pulmonary artery smooth muscle cells. Scientific Reports, 8, 12972.

+ Open protocol

+ Expand

Measuring Plasma Inflammatory Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma IL-6 (R&D Systems, Minneapolis, MN), TNF-alpha (R&D Systems, Minneapolis, MN), CRP (R&D Systems, Minneapolis, MN), norepinephrine (Abnova, Taiwan) and hepcidin (R&D Systems, Minneapolis, MN) was measured by enzyme linked immunosorbent assay. All samples were run in duplicate following the manufacturer’s protocol.

Apple C.G., Miller E.S., Loftus T.J., Kannan K.B., Parvataneni H.K., Hagen J.E., Efron P.A, & Mohr A.M. (2019). Impact of Injury Severity on the Inflammatory State and Severe Anemia. The Journal of surgical research, 248, 109-116.

+ Open protocol

+ Expand

Comprehensive Vitamin D and Inflammatory Biomarkers Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma vitamin D (25-OH) Total Human Assay was used to quantify levels of total vitamin D (25-OH) in plasma (Crystal Chem, Elk Grove Village, IL). The reference range for vitamin D (25-OH) is 25–80 ng/mL. Plasma erythropoietin (EPO)(R&D, Minneapolis, MN), interleukin-6 (IL-6)(R&D, Minneapolis, MN), tumor necrosis factor-alpha (TNF-α)(R&D, Minneapolis, MN), C-reactive protein (CRP)(R&D, Minneapolis, MN), iron (Abcam, Cambridge, MA), hepcidin (R&D, Minneapolis, MN), transferrin (Thermofisher, Waltham MA) and ferritin (Abcam, Cambridge UK) were measured by enzyme linked immunosorbent assay. The reference ranges for these assays are as follows: EPO 4.1–19.5miU/mL, IL-6 0–16.4 pg/mL, TNF-α 0–29.4 pg/mL, CRP 0–1240 ng/mL, iron 60–170 ug/dL, hepcidin 1–55 ng/mL, transferrin 170–370 ug/mL, ferritin 12–300 ng/mL. All samples were run in duplicate following the manufacturer’s protocol.

Apple C.G., Miller E.S., Kannan K.B., Stortz J.A., Cox M., Loftus T.J., Parvataneni H.K., Patrick M., Hagen J.E., Brakenridge S., Efron P.A, & Mohr A.M. (2020). VITAMIN D STATUS IS ASSOCIATED WITH HEPCIDIN AND HEMOGLOBIN CONCENTRATIONS IN PATIENTS WITH SEVERE TRAUMATIC INJURY. The journal of trauma and acute care surgery, 89(6), 1124-1130.

+ Open protocol

+ Expand

Monitoring Kidney Transplant Outcomes

Check if the same lab product or an alternative is used in the 5 most similar protocols

All patients were regularly followed for 1 year. Clinical information was obtained from electronic medical records, including demographics, historical data, transplantation details and medications. Routine examinations, including complete blood count, Hb, creatinine, EPO, ferritin, transferrin, total iron binding capacity (TIBC), and other parameters were measured in the central laboratory of West China Hospital using standard laboratory methods. Estimated GFR (eGFR) was calculated using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation. Fasting blood samples of KTRs were collected in the morning at week 2 and month 3 for measuring serum intact fibroblast growth factor 23 (iFGF23; Boster, Wuhan, China) and hepcidin (R&D system, USA) concentrations using enzyme-linked immunosorbent assay (ELISA) methods with available commercial kits.

Li H., Hu S.M., Li Y.M., Ciancio G., Tadros N.N., Tao Y., Bai Y.J, & Shi Y.Y. (2022). Beneficial effect of roxadustat on early posttransplant anemia and iron utilization in kidney transplant recipients: a retrospective comparative cohort study. Annals of Translational Medicine, 10(24), 1360.

+ Open protocol

+ Expand

Blood Sampling for Substrate Oxidation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood sampling occurred after a 10‐h overnight fast. Blood collections assessing Hgb and Hct for safety were conducted using venipuncture. During the substrate oxidation tests, blood was collected using an indwelling catheter kept patent using IV saline at approximately −100, −20, 0, 20, 40, 60, and 80 min for assessment of glucose isotope enrichments (6,6‐[²H₂] glucose, Cambridge Isotope Laboratories, Andover, MA; cat: DLM‐349‐MPT‐PK). Blood collected at −100 and 80 min was also used to assess IL‐6 and hepcidin concentrations (R&D Systems, Inc, Minneapolis, MN; cat: D6050 and DRG International, Springfield, NJ; cat: EIA‐5782 respectively). Tubes yielding serum were allowed to clot at room temperature (RT) for at least 10 min then centrifuged at 3600 rpm for 10 min at 4°C. Blood was then stored at −80°C until analyzed.

Drummer D.J., McNiff J.L., Howard E.E., Gwin J.A., Carrigan C.T., Murphy N.E., Wilson M.A., Michalak J., Ryan B.J., McClung J.P., Pasiakos S.M, & Margolis L.M. (2024). Exogenous erythropoietin increases hematological status, fat oxidation, and aerobic performance in males following prolonged strenuous training. Physiological Reports, 12(10), e16038.

+ Open protocol

+ Expand

Iron Metabolism Biomarkers in Anemia

Check if the same lab product or an alternative is used in the 5 most similar protocols

The first blood sampling moment in all groups was on ICU admission. The second sampling moment for AI patients was on the day they developed anemia, the third sampling moment was 2 days later. Control patients were sampled on the first and third day of complying to their classification (Additional file 1: Table S1).
Samples were centrifuged at 1500 g for 15 min at room temperature and plasma was stored at − 80 °C. Measurements were done in heparin anti-coagulated plasma. Serum iron, transferrin, ferritin and haptoglobin were measured by immunoturbidimetric methods (Roche Cobas c702). Transferrin saturation was calculated by the formula serum iron/(25.2 × transferrin). Hepcidin (R&D), soluble transferrin receptor (sTfR) (Biovendor), erythroferrone (MyBioSource) and IL-6 levels (R&D) were measured by enzyme-linked immunosorbent assay kits.

Boshuizen M., Binnekade J.M., Nota B., van de Groep K., Cremer O.L., Tuinman P.R., Horn J., Schultz M.J., van Bruggen R, & Juffermans N.P. (2018). Iron metabolism in critically ill patients developing anemia of inflammation: a case control study. Annals of Intensive Care, 8, 56.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!