PC12 cells were plated onto 24‐well culture plates (ca. 105 per well) and incubated for 24 h. Cells were double stained with Fluor647‐conjugated annexin V and propidium iodide (4A Biotech Co. Ltd., Beijing, China), and apoptosis was detected by flow cytometry (Cytoflex, Beckman, USA).
Propidium iodide
Manufactured by 4A Biotech
Sourced in China
Propidium iodide is a fluorescent dye that binds to the DNA of cells. It is commonly used in flow cytometry and other cell analysis techniques to stain and quantify the DNA content of cells.
Automatically generated - may contain errors
Lab products found in correlation
Rnase a, by 4A Biotech (5 mentions)
Fluorescence activated cell sorting flow cytometry, by Beckman Coulter (2 mentions)
Annexin 5 pi detection kit, by 4A Biotech (2 mentions)
Flow cytometry, by Beckman Coulter (2 mentions)
Cytoflex, by Beckman Coulter (1 mentions)
Facscan, by BD (1 mentions)
Annexin 5 fitc pi, by Keygen Biotech (1 mentions)
Facscalibur, by BD (1 mentions)
Cellquest pro, by BD (1 mentions)
Modfit lt v5, by Verity Software House (1 mentions)
Annexin 5 fitc, by 4A Biotech (1 mentions)
Ow cytometry, by Beckman Coulter (1 mentions)
Uorescence activated cell sorting ow cytometry, by Beckman Coulter (1 mentions)
11 protocols using propidium iodide
1
Apoptosis Quantification in PC12 Cells
2
Cell Cycle and Apoptosis Assay
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Cells were seeded into 6-well plates and treated with sorafenib for 24 h. 3 × 105 treated cells were seeded into 6-well plates and cultured for 48 h at 37°C to assess the cell cycle and apoptosis. The cells for cell cycle analysis were digested using trypsin (Hyclone), washed twice with phosphate-buffered saline (PBS), and fixed in 70% ethanol overnight at 4°C. Then the cells were centrifuged at 500 × g for 5 min, washed twice with cold PBS, and centrifuged. Cell cycle analysis was performed through fluorescence-activated cell sorting flow cytometry (Beckman Coulter, Palo Alto, CA, USA) after treating the cells with RNase A (0.1 mg/mL) and propidium iodide (PI, 0.05 mg/mL) purchased from 4A Biotech (Beijing, China) for 30 min at 37°C. Following the instructions of the manufacturer, cells were harvested and were stained with Annexin V-FITC/PI (KeyGEN Biotech, Nanjing, China) for the analysis of apoptosis. Then the cells were acquired by flow cytometry (FACScan, BD Biosciences, USA) and analyzed by FlowJo 7.6.1.
3
Apoptosis Analysis of LINC01234 CEC2 Cells
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After 48 h transfection, cells were trypsinized and harvested from six-well plates then collected after centrifugation. Cell pellets were washed with PBS (cold) and then cells were stained with FITC-Annexin V and propidium iodide (4A Biotech, China). Viable and dead cells, as well as early & late apoptotic cells were quantified and analyzed by FACS Calibur and Cell Quest Pro software (BD Bioscience, USA). The negative control group was used to compare relative ratios of the early and late apoptotic cells with knockdown LINC01234 CEC2 cells.
4
Cell Cycle Analysis of Adherent CRC Cells
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Adherent CRC cells were treated with CVB-D (30 µM) at 37°C for 48 h, then harvested and fixed in 75% ethanol at 4°C overnight. The cells were digested and stained using RNase and propidium iodide (4A Biotech), respectively. The cells were stained at 37°C for 30 min in the dark. The cell cycle was analyzed using a flow cytometer. ModFit LT V5.0 software (Verity Software House, Inc.) was used for analysis. Three biologically independent experiments were conducted.
5
Cell Cycle Analysis by Flow Cytometry
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To assess the cell cycle, we seeded 3 × 105 treated cells into six-well plates and incubated them at 37 °C for 48 h. For the cell cycle analysis, cells were digested with trypsin (Hyclone, Logan, UT, USA), washed twice with phosphate-buffered saline (PBS), and fixed in 70% ethanol at 4 °C overnight. The cells were centrifuged at 500× g for 5 min, washed twice with cold PBS, and centrifuged. Cell cycle analysis was performed using fluorescence-activated cell sorting after the digested cells were treated with RNase A (0.1 mg/mL) and stained with propidium iodide (0.05 mg/mL; 4A Biotech, Beijing, China) for 30 min at 37 °C.
6
Cell Cycle and Apoptosis Analysis
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For cell cycle analysis, cells were harvested and washed with ice-cold PBS, then fixed by 70% ice-cold ethanol over night at 4°C, nuclear stained with propidium iodide (PI). Cell suspension was immediately analyzed by flow cytometer. Data analysis was analyzed by ModFit software. For cell apoptosis analysis, cells were stained with Annexin V-FITC (10 min) and propidium iodide (5 min) (4A Biotech) at room temperature in the dark. Data analysis was analyzed by CytExpert 2.0 software.
7
Cell Cycle and Apoptosis Analysis
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For analysis of the cell cycle and apoptosis, 3 × 105 treated cells were seeded in 6-well plates and cultured for 48 h at 37°C. For cell cycle analysis, the cells were digested using trypsin (HyClone, Logan, UT, United States), washed twice with phosphate-buffered saline (PBS), and fixed in 70% ethanol overnight at 4°C. The cells were centrifuged at 500 ×g for 5 min, washed twice with cold PBS, and centrifuged. After the cells were treated with RNase A (0.1 mg/ml) and propidium iodide (PI, 0.05 mg/ml, 4A Biotech, Beijing, China) for 30 min at 37°C, cell cycle analysis was performed using fluorescence-activated cell sorting flow cytometry (Beckman Coulter, Palo Alto, CA, USA). For analysis of apoptosis, the cells were trypsinized followed by two PBS washes. The cells were stained using the Annexin V/PI detection kit (4A Biotech) for 5 min at 25°C. Apoptotic cells were measured using flow cytometry (Beckman Coulter). All experiments were repeated at least three times.
8
Cell Cycle and Apoptosis Analysis
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To assess the cell cycle and apoptosis, we seeded 3 × 105 treated cells into six-well plates and incubated them at 37° C for 48 h. For the cell cycle analysis, cells were digested with trypsin (Hyclone), washed twice with phosphate-buffered saline (PBS) and fixed in 70% ethanol at 4° C overnight. The cells were centrifuged at 500 x g for 5 min, washed twice with cold PBS and centrifuged. Cell cycle analysis was performed using fluorescence-activated cell sorting after the digested cells were treated with RNase A (0.1 mg/mL) and stained with propidium iodide (0.05 mg/mL; 4A Biotech, Beijing, China) for 30 min at 37° C.
For the apoptosis analysis, cells were digested with trypsin and washed twice with PBS. The cells were then stained for 5 min at room temperature using an Annexin V/propidium iodide detection kit (4A Biotech). Apoptotic cells were measured using flow cytometry (Beckman Coulter). All experiments were repeated at least three times.
For the apoptosis analysis, cells were digested with trypsin and washed twice with PBS. The cells were then stained for 5 min at room temperature using an Annexin V/propidium iodide detection kit (4A Biotech). Apoptotic cells were measured using flow cytometry (Beckman Coulter). All experiments were repeated at least three times.
9
Cell Cycle Analysis of ccRCC Cells
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ccRCC cells were treated with CVB (40 μM) in a 6-well plate for 48 h. Cells were then collected, washed twice in cold PBS and fixed in 70% ethanol at 4 °C overnight. The next day, the fixed cells were washed with PBS again and then stained with propidium iodide (PI) (40 µg/ml) and RNase A (2.5 mg/ml, 4A Biotech, Beijing, China) at 37 °C in the darkroom for 30 min. A flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) was used to detect the cell cycle distribution. The assays were repeated three times.
10
Cell Cycle and Apoptosis Analysis
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To assess the cell cycle and apoptosis, 3×105 treated cells were seeded into 6-well plates and cultured for 48h at 37°C. The cells for cell cycle analysis were digested using trypsin (Hyclone), washed twice with phosphate-buffered saline (PBS), and xed in 70% ethanol overnight at 4°C. The cells were centrifuged at 500g for 5min, washed twice with cold PBS, and centrifuged. After treating with RNase A (0.1mg/ml) and propidium iodide (PI, 0.05mg/ml) purchased from 4A Biotech (Beijing, China) for 30min at 37°C, cell cycle analysis was performed through uorescence-activated cell sorting ow cytometry (Beckman Coulter, Palo Alto, CA, USA). For the analysis of apoptosis, cells were trypsinised followed by two PBS washing steps. The cells were stained using the Annexin V/PI detection kit (4A Biotech, Beijing, China) for 5min at room temperature. The apoptotic cells were measured using ow cytometry (Beckman Coulter). All experiments were repeated at least three time.
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