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2 protocols using agno3

1

Synthesis and Characterization of Metal-Organic Complexes

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1,3,5-Benzenetricarboxylic chloride (TMC, 98%) and anhydrous piperazine (PIP, 99%) were purchased from Alfa. l-cystine (99%), l-cysteine (99%), AgNO3 (99%), Pb(NO3)2 (99%), and NiCl2·6H2O (99%) were obtained from Adamas-beta. Hg(NO3)2 (99%) was received from Xiya Reagent. n-hexane, Cd(NO3)2·4H2O, CuCl2·2H2O, NaOH, HCl (36.0–38.0%), Na2SO4, MgSO4, NaCl, and CaCl2 were bought from Kelong Chemical Reagent Factory (Chengdu, China). All reagents were directly used without further purification. Flat-sheet non-woven-reinforced polysulfone (PSf) ultrafiltration membrane with a molecular weight cut-off of 60 000 as the support was provided by Beijing Haiqingyuan Tech. Co. Ltd and deionized water (3 μS cm−1) was obtained from an ultrapure water treatment system.
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2

Fabrication and Characterization of Heavy Metal Nanoparticles

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Chicken eggs were bought from the local market. K2SO4, AgNO3, SnCl2·4H2O, BaCl2, Pb(NO3)2, CuSO4·5H2O, MgSO4·7H2O, MnSO4·H2O, FeCl2·4H2O, FeCl3, ZnCl2, NaCl,CaCl2, L-alanine, L-lysine, L-phenylalanine, L-cysteine, L-arginine, L-glycine, L-aspartic acid, L-glutamine, glucose, fructose, urea and glutathione (GSH) were purchased from Adamas-Beta Co., Ltd. (Shanghai, China). Dopamine hydrochloride and tris(hydroxymethyl)aminomethane (Tris) were obtained from 9 Ding Chemistry Co., Ltd. (Shanghai, China). 96-well plates (In vitro scientific) were obtained from Xinyou Biotechnology Co., Ltd. (Hangzhou, China). 6-well plates were obtained from Nest Biotechnology Co., Ltd. (Wuxi, China). Black opaque 96-well plates were supported by Jet Bio-Filtration Co., Ltd. (Guangzhou, China). Dulbecco’s modified Eagle’s medium (DMEM), trypsin, penicillin, streptomycin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), propidium iodide (PI) and Calcein-AM were bought from Solarbio Science & Technology Co., Ltd. (Beijing, China). Fetal bovine serum (FBS) was purchased from Zhejiang Tianhang Sijiqing Biotechnology Co., Ltd., (Hangzhou, China). All of the chemicals were analytical grade and used without further purification.
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