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Gsk2334470

Manufactured by Merck Group
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GSK2334470 is a laboratory equipment product. It is designed to perform specific functions in a research or testing environment. A detailed description of its core function is not available at this time while maintaining an unbiased and factual approach.

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Lab products found in correlation

Mitotracker orange cmtmros, by Thermo Fisher Scientific (1 mentions) 1 1 dihexadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate diic16 3, by Thermo Fisher Scientific (1 mentions) Pd98059, by Merck Group (1 mentions) Torin1, by Bio-Techne (1 mentions) Ly294002, by Merck Group (1 mentions) Akti 1 2, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Pp242, by Merck Group (1 mentions) P akt thr308, by Cell Signaling Technology (1 mentions) P akt ser473, by Cell Signaling Technology (1 mentions) Cell culture materials, by Thermo Fisher Scientific (1 mentions) Total akt, by Cell Signaling Technology (1 mentions)

4 protocols using gsk2334470

1

Molecular Compound Dissolution and Staining

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Various small molecules were purchased for this study and dissolved in dimethyl sulfoxide (DMSO) solution: GSK2334470 (Sigma), Gö6893 (Calbiochem), PF4708671 (Torcis), Akt inhibitor X (Calbiochem), Akt inhibitor VI (H-AVTDHPDRLWAWEKF-OH; Calbiochem), LY294002 (Calbiochem), and rapamycin (Calbiochem). Cellular staining reagents were purchased from Molecular Probes (Fisher Scientific), which included 1,1’-dihexadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiIC16(3); Cat# D384), and MitoTrackerOrange CMTMRos (Cat# M7510).
Schmitt D.L., Sundaram A., Jeon M., Luu B.T, & An S. (2018). Spatial alterations of De Novo purine biosynthetic enzymes by Akt-independent PDK1 signaling pathways. PLoS ONE, 13(4), e0195989.
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2

Preparation of Kinase Inhibitor Solutions

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PDK1 inhibitor GSK2334470, MEK inhibitor PD98059 and RSK inhibitor BI-D1870 were purchased from Sigma-Aldrich and dissolved in DMSO to a final concentration of 10 mM. The obtained solutions were aliquoted and stored at −80 °C.
Somale D., Di Nardo G., di Blasio L., Puliafito A., Vara-Messler M., Chiaverina G., Palmiero M., Monica V., Gilardi G., Primo L, & Gagliardi P.A. (2020). Activation of RSK by phosphomimetic substitution in the activation loop is prevented by structural constraints. Scientific Reports, 10, 591.
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3

Inhibiting PI3K-mTOR Pathway in NK Cells

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Antibodies recognizing mouse CD3 (mAb 145-2C11), NKp46 (mAb 29A1.4), NK1.1 (PK136), CD117 (2B8), CD127 (A7R37), Ly49A (A1), Ly49C/I (5E6), CD135 (A2F10), SCA-1 (D7), NKG2D (A10), NKG2A/C/E (20D5), CD11b (mAb M1/70), CD27 (LG.7F9), and isotype controls were purchased from eBioscience or BD. CD1d-PBS157 tetramer was provided by the National Institutes of Health tetramer facility. To inhibit the activities of PI3K–mTOR pathway, NK cells were treated with various inhibitors: 1 µM Torin1 (Tocris), 1 µM GSK2334470 (Sigma-Aldrich), 1 µM AKTi1/2 (EMD Millipore), or 10 µM Ly294002 (EMD Millipore).
Yang M., Li D., Chang Z., Yang Z., Tian Z, & Dong Z. (2015). PDK1 orchestrates early NK cell development through induction of E4BP4 expression and maintenance of IL-15 responsiveness. The Journal of Experimental Medicine, 212(2), 253-265.
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4

HUVEC Molecular Pathway Regulation

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Primary human umbilical vein endothelial cells (HUVECs) were from the institute of life science, Chongqing Medical University (Chongqing, China). Cell culture materials were purchased from Invitrogen (Carlsbad, CA, USA). Growth media were obtained from Gibco (Grand Island, NY, USA). GSK2334470 and PP242 were products of Sigma (St. Louis, MO, USA). Antibodies against p‐eNOS Ser1177, total‐Akt, p‐Akt Ser473, p‐Akt Thr308, PDK1, and SIN1 were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against total‐eNOS, p‐eNOS Thr495, and β‐actin were obtained from Millipore (Temecula, California).
Liang X., Wang R., Guo Y., Cheng Z., Lv D., Luo M., He A., Luo S, & Xia Y. (2021). Phosphorylation of Akt at Thr308 regulates p‐eNOS Ser1177 during physiological conditions. FEBS Open Bio, 11(7), 1953-1964.
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