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3 protocols using dicyclohexylcarbodimide

1

Synthesis and Characterization of Resveratrol Derivatives

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The solvents used: acetone, ethanol, methanol, n-hexane, chloroform, 2-propanol, and ethyl ether were purchased from Carlo Erba Reagenti (Milan, Italy). Chloroform was purified by standard procedures. Resveratrol or 3,5,4’-trihydroxy-trans-stilbene (MM = 22,825 g/mol), D-Mannose (MM = 180,156 g/mol), ciprofloxacin (MM = 331,346), methacrylic acid (MAA), 85% formic acid (FA), 85% phosphoric acid (AP), sodium nitrite (SN), dicyclohexylcarbodimide (DCC), dimethylaminopyridine (DMAP), dimethyl sulfoxide (DMSO), potassium chloride (KCl), hydrochloric acid (HCl), sodium phosphate (Na3PO4), citric acid, disodium hydrogen phosphate (Na2HPO4), sorbitan monooleate (Span 80) and polyoxyethylene sorbitan monooleate (Tween 80), N,N′-ethylene bis-acrylamide (EBA) were purchased from Sigma-Aldrich (Sigma Chemical CO, St. Louis, MO, USA).
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2

Synthesis of Functionalized Nanoparticles

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The purchased solvents are of High-Performance Liquid Chromatography (HPLC) grade and obtained from Fisher scientific, UK, through a local supplier. Dicyclohexyl carbodimide (DCC), 4-dimethyl aminopyridine (DMAP), ammonium hydroxide (NH4OH), cinnamic acid (CA), 3-aminopropyl silane (APT), ferrous sulfate heptahydrate (FeSO4·7H2O), ferric sulfate hexahydrate (Fe2(SO4)3·6H2O), lactobionic acid (LBA), Hesperidin (HES), Amphotericin B (AmpB), and curcumin (CUR) were purchased from Sigma Aldrich through a local supplier.
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3

Synthesis and Characterization of Functionalized Magnetic Nanoparticles

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All organic solvents utilized in experiments were of HPLC grade. Ferric sulphate hexahydrate Fe2(SO4)3.6H2O, ferrous sulphate heptahydrate (FeSO4.6H2O), 3-(trimethoxysilyl)propyl methacrylate (MPTES), 4-dimethyl amino pyridine (DMAP), azobisisobutyronitrile (AIBN), ammonium hydroxide, dicyclohexylcarbodimide (DCC), isoniazid and amphotericin B were obtained from Sigma-Aldrich (San Francisco, CA, USA) and Merck (Darmstadt, Germany).
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