The experiment was conducted in a completely randomized design in a 2 × 2 factorial scheme: parasitoids from two hosts (E. heros and D. melacanthus) × two genera of the parasitoid (male and female) and 10 replicates. Ten females and 10 males of T. urichi progeny were analyzed for each host. For each parasitoid, morphometric measurements of the right anterior wing length and width, right posterior tibia length, and body length (head to the end of the abdomen) were performed, according the standardized quality control procedures established by the International Organization of Biological Control (Global IOBC Working Group: ‘Quality Control of Mass Reared Arthropods’)26 . For the evaluation of these morphological characters, each specimen was photographed with a stereoscopic microscope (Leica Application Suite – Version 1.6.0) and the morphometry measured using Image J (Version 1.47)25 (link).
Application suite version 1
Manufactured by Leica
The Application Suite – Version 1.6.0 is a software package developed by Leica to provide a comprehensive set of tools for the analysis and processing of data generated by Leica's lab equipment. The core function of this software is to offer a unified interface for controlling and managing various Leica instruments, as well as to facilitate the storage, visualization, and analysis of the acquired data.
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Lab products found in correlation
3 protocols using application suite version 1
1
Morphometric Analysis of Parasitoid Wasps
2
Egg Morphometric Measurements of Three Assassin Bug Species
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This trial was conducted in a completely randomized design with three treatments (eggs of E. heros, D. melacanthus, and P. nigrispinus) and 10 replicates (a single egg per replicate). The evaluated parameters were egg width and length. To measure these parameters, the eggs were first photographed using a stereomicroscope (Leica Application Suite-Version 1.6.0) and later measured using the software Image J-Version 1.47.
3
Morphometric Analysis of Parasitoid Hosts
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The experiment was carried out in a 3 × 2 factorial completely randomized design; 3 parasitoid hosts (E. heros, D. melacanthus, and P. nigrispinus eggs) × 2 parasitoid genders (female and male). Fifteen replicate adults of both genders were measured individually. Thus, 15 males and 15 females were measured for each parasitoid host, totaling 90 adults in bioassay 1. Length and width of the right anterior wing, length of the right hind tibia, and body length (head to tip of the abdomen) were measured in each replicate (adult insect). To measure the morphological characters, each specimen was photographed using a stereoscopic microscope (Leica Application Suite, Version 1.6.0). Images were used for morphometric analysis with the software Image J (Version 1.47) (Queiroz et al 2017) (link).
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