Anti nlrp3 catalog no orb101128 antibody

EA (purity > 95%), LPS (Escherichia coli O111:B4), 6-hydroxydopamine (6-OHDA), and apomorphine hydrochloride were obtained from Sigma-Aldrich (St. Louis, CA, USA). Enzyme-linked immunosorbent assay (ELISA) kits for TNF-α, IL-1β, and IL-18 were bought from Elabscience Biotechnology Co., Ltd. (Wuhan, China). The MTT assay kit was from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China). Small interfering RNA (siRNA) against NLRP3 was purchased from GenePharma (Shanghai, China). Anti-CR3 complement receptor (OX-42 Catalog No. Ab1211) and tyrosine hydroxylase (TH, Catalog No. Ab113) antibodies were bought from Abcam (Cambridge, MA, USA). Anti-caspase-1 (Catalog No. 22915-1-AP), ionized calcium-binding adapter molecule-1 (Iba-1, Catalog No. 10904-1-AP), β-actin (Catalog No. 20536-1-AP), TNF-α (Catalog No. 17590-1-AP), IL-1β (Catalog No. 66737-1-Ig), IL-18 (Catalog No. 10663-1-AP), rabbit IgG (Catalog No. SA00001-2), and mouse IgG (Catalog No. SA00001-1) antibodies were purchased from the Proteintech Group (Chicago, IL, USA). Anti-NLRP3 (Catalog No. orb101128) antibody was purchased from Biorbyt (Cambridge, United Kingdom).

Naringenin and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). MTT assay kit was obtained from Beijing Solarbio science and Technology Co., Ltd (Beijing, China). Enzyme-linked Immunosorbant assay (ELISA) kits for TNF-α, IL-1β, and IL-18 were bought from Elabscience Biotechnology Co., Ltd (Wuhan, China). Dulbecco's modified Eagle medium (DMEM) and OPTI-MEM were obtained from Gibco-BRL (Invitrogen Life Technologies, Carlsbad, USA). Anti-tyrosine hydroxylase (TH, Catalog No. Ab41528) and OX-42 (Catalog No. Ab1211) antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-caspase-1 (Catalog. No. 22915-1-AP), ionized calcium-binding adapter molecule-1 (Iba-1, Catalog. No. 10904-1-AP), β-actin (Catalog. No. 20536-1-AP), Rabbit IgG (Catalog. No. SA00001-2), and mouse IgG (Catalog. No. SA00001-1) antibodies were purchased from Proteintech Group (Chicago, IL, USA). Anti-NLRP3 (Catalog. No. orb101128) antibody was purchased from Biorbyt (Cambridge, United Kingdom). Anti-ASC (Catalog. No. 67824) antibody was obtained from Cell Signaling Technology, Inc. (Beverly, MA, USA). The small interfering RNA (siRNA) against NLRP3 was purchased from GenePharma (Shanghai, China).