Mouse monoclonal anti p nf κb sc 271908

Proteinase K and PBS tablets were obtained from MP Biomedicals (Irvine, CA, USA). Hydrogen peroxide (H₂O₂), formaldehyde, trichloroacetic acid (TCA), reduced glutathione (GSH), N-(1-Naphthyl) ethylenediamine dihydrochloride, 5,5′-dithiobis-(2-nitro benzoic acid) (DTNB), and 1-chlor-2,4-dinitrobenzene (CDNB) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Mouse monoclonal anti-p-NF-κB (SC-271908), mouse monoclonal anti-p-JNK (SC-6254), mouse monoclonal anti-TNF-α (SC-52B83), mouse monoclonal anti-COX-2 (SC-514489), rabbit polyclonal anti-Nrf2 (SC-722), mouse monoclonal anti-TLR4 (SC-293072), mouse anti-Iba-1 (sc-32725), and mouse monoclonal anti-HO-1 (SC-13691), along with Avidin-biotin complex (ABC kit, SC-516216) and 3,3′-Diaminobenzidine (DAB, SC-216567), were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). The horseradish peroxidase-conjugated secondary antibodies (ab-6789, ab-6721) and mounting media (ab-10431) were purchased from Abcam UK. p-NF-κB ELISA kit (Cat # SU-B28069), Nrf2 ELISA kit (Cat # SU-B30429), HO-1 ELISA kit (Cat # SH-032529), and TNF-α ELISA kit (Cat # SU-B3098) were purchased from Shanghai Yuchun Biotechnology (Shanghai, China). ATRA (All trans-retinoic acid) of the highest analytical grade (99% HPLC) was purchased from the local pharmaceutical industry (GlaxoSmithKline, Islamabad, Pakistan).

Carveol (#192384, PubChem ID:24851543), a mixture of isomers, with 97% purity, and 3,3-diaminobenzidine tetrahydrochloride hydrate (#D5637, PubChem ID:57654109) were purchased from Sigma-Aldrich (USA). Mouse monoclonal anti-p-NF-κB (SC-271908), mouse monoclonal anti-TNF-α (SC-52B83), mouse monoclonal anti-HO-1 (SC-136960), rabbit polyclonal anti-Nrf2 (SC-722), mouse monoclonal anti-VEGF (SC-7269), and ABC Elite kit (SC-516216) were purchased from Santa Cruz Biotechnology, Dallas, TX, USA). Rat ELISA kits p-NF-κB (SU-B28069) and TNF-α ELISA kit (SU-B3098) were procured from Shanghai Yuchun Biotechnology, Shanghai, China, while rat COX-2 (E-EL-M0959) was purchased from Elabscience Biotechnology Inc., Houston, TX, USA. The horseradish peroxidase-conjugated secondary antibody (ab-6789) was obtained from Abcam (Cambridge, UK). Proteinase K (#02193981-CF) was obtained from MP Bio USA. All other solvents and reagents as DPX Mounting media (#06522), 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB, #D8130, PubChem ID:24894189), trichloroacetic acid (TCA, #T6399, PubChem ID:24900373), and N-(1-naphthyl) ethylenediamine dihydrochloride (#222488, PubChem ID:24853334) were procured from Sigma-Aldrich (St. Louis, MO, USA).