Mouse brain tissue or human tissue samples were lysed in RIPA lysis buffer (Cell signaling), supplemented with protease inhibitors cocktail and phosphatase inhibitor (Thermo Fisher Scientific), and kept on ice for 30 mins. The lysates were then centrifuged at 14,000 ×g for 30 mins at 4°C. The supernatant protein concentration was measured and normalized between samples, and was boiled in SDS loading buffer. Prepared samples were subjected to 4%–20% SDS-PAGE (Bio-Rad laboratories).
Protease inhibitors cocktail and phosphatase inhibitor
Manufactured by Thermo Fisher Scientific
Protease inhibitor cocktail is a mixture of compounds designed to inhibit the activity of proteolytic enzymes. Phosphatase inhibitor cocktail is a mixture of compounds designed to inhibit the activity of phosphatase enzymes. Both products are commonly used in biological research applications to preserve the integrity of target proteins.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using protease inhibitors cocktail and phosphatase inhibitor
1
Tissue Protein Extraction and Quantification
2
Tissue Protein Extraction and Quantification
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Mouse brain tissue or human tissue samples were lysed in RIPA lysis buffer (Cell signaling), supplemented with protease inhibitors cocktail and phosphatase inhibitor (Thermo Fisher Scientific), and kept on ice for 30 mins. The lysates were then centrifuged at 14,000 ×g for 30 mins at 4°C. The supernatant protein concentration was measured and normalized between samples, and was boiled in SDS loading buffer. Prepared samples were subjected to 4%–20% SDS-PAGE (Bio-Rad laboratories).
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