Cellsens dimension 1.9 digital imaging software

An absorbable gelatin surgical sponge (Surgispon, Aegis Lifesciences, India) was cut manually with a blade into pieces of 9 mm³ (3 × 3 × 1 mm). The 1 × 10⁶ cells were resuspended in 20 µl of the rat tail collagen, type I (Gibco, USA) (in the control group), and MgDCA or NaDCA salt (Sigma-Aldrich GmbH, Germany) dissolved in a medium (investigational medicine-treated groups). A 20 µl liquid mixture of tumor cells was pipetted onto a piece of a sponge. The NaDCA- or MgDCA-treated tumor groups and their controls were formed. At the EDD7, the tumor was grafted onto CAM among significant blood vessels. Its structural changes were observed with a stereomicroscope (SZX2-RFA16, Olympus, Japan) in vivo during the EDD9–12 period. The tumor images were acquired using a digital camera (DP92, Olympus, Japan) and CellSens Dimension 1.9 Digital Imaging Software.

Formalin-fixed and paraffin-embedded samples of approximately 0.5 × 2.5 cm in size from each CAM with LSCC implant were sliced into 3 μm thick sections and stained with hematoxylin and eosin (H&E) for histological and morphometric evaluation. Histological evaluation of the samples was performed with the cold light microscope OLYMPUS BX40F4 (Olympus Optical Co. Ltd., Japan) under 10x magnification using CellSens Dimension 1.9 Digital Imaging Software for Research Applications (Olympus Corporation of the Americas, USA).