MalI (10 μM) was incubated with 100 μM AHOAP in air-saturated buffer A supplemented with 2 mM ascorbate, 10 μM Fe2+, and 1 mM α-KG at 25 °C. After 30 min, 10 mM NADPH, 10 mM ZnCl2, and 20 μM MalB were added to the reaction. At each time point, an aliquot (10 μL) was removed from the reaction, mixed with an equal volume of acetonitrile, and clarified using centrifugation. Then, 10 μL of the supernatant was injected into and analyzed using HPAEC using a DNAPac PA100 4 × 250 mm column (Thermo Scientific) monitored at 260 nm.
Dnapac pa100 4 250 mm column
Manufactured by Thermo Fisher Scientific
The DNAPac PA100 4 × 250 mm column is a high-performance liquid chromatography (HPLC) column designed for the separation and purification of DNA fragments, oligonucleotides, and other biomolecules. The column features a polymeric anion-exchange stationary phase that provides efficient and reproducible separation of these analytes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dnapac pa100 4 250 mm column
1
Enzymatic Assay for MalI Activity
2
Nucleotide Quantification by HPAEC
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