Capsazepine

Manufactured by Cayman Chemical

Sourced in United States

Capsazepine is a synthetic compound used as a research tool. It functions as a competitive antagonist of the capsaicin receptor (TRPV1), a non-selective cation channel that plays a role in the perception of pain and temperature.

Automatically generated - may contain errors

Lab products found in correlation

13 protocols using capsazepine

Cannabinoid Modulation of Fear Extinction

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The AEA hydrolysis inhibitor URB597 (0.3 mg/kg; Cayman Chemical, Cedarlane®, Burlington, ON, Canada), the 2-AG hydrolysis inhibitor MJN110 (10 mg/kg; provided by B.F. Cravatt), the CB1R antagonist/inverse agonist AM251 (1 mg/kg; Tocris, Cedarlane®, Burlington, ON, Canada), the TRPV1R antagonist Capsazepine (CPZ; 5 mg/kg; Cayman Chemical, Cedarlane®, Burlington, ON, Canada) or their vehicle (5% polyethylene glycol, 5% Tween-80, 90% saline) were injected i.p. at a volume of 1 ml/kg. URB597, MJN110 or their vehicle were injected 60 min before the extinction training session, AM251, CPZ or their vehicle were injected 90 min before the extinction training session.
Doses and timing were chosen based on previously published papers (Kathuria et al., 2003 (link); Colangeli et al., 2017 (link); Ratano et al., 2017 (link); Morena et al., 2018 (link); Sticht et al., 2019 (link)) and pilot experiments performed in our laboratory. All drug solutions were freshly prepared before each experiment.

Morena M., Nastase A.S., Santori A., Cravatt B.F., Shansky R.M, & Hill M.N. (2021). Sex-dependent effects of endocannabinoid modulation of conditioned fear extinction in rats. British journal of pharmacology, 178(4), 983-996.

+ Open protocol

+ Expand

Inhibition of STAT3/5 by SH-4-54

Check if the same lab product or an alternative is used in the 5 most similar protocols

SH-4-54, a novel small molecule STAT3/5 inhibitor [10 (link)], was reconstituted in DMSO at a 25 mM stock. Individual aliquots were stored at -20°C, and cells were treated with vehicle or an appropriate concentration of drug (initially at 10 μM, followed by a 5 μM maintenance dose). Recombinant human prolactin (Cedarlane) was used at 100 ng/ml. Capsazepine (Cayman Chemical), paclitaxel (Sigma-Aldrich), and bleomycin (Sigma-Aldrich) were reconstituted in DMSO and used at final concentrations corresponding to 25 μM, 0.1 nM, and 1500 mU, respectively.

Linher-Melville K., Nashed M.G., Ungard R.G., Haftchenary S., Rosa D.A., Gunning P.T, & Singh G. (2016). Chronic Inhibition of STAT3/STAT5 in Treatment-Resistant Human Breast Cancer Cell Subtypes: Convergence on the ROS/SUMO Pathway and Its Effects on xCT Expression and System xc- Activity. PLoS ONE, 11(8), e0161202.

+ Open protocol

+ Expand

Pharmacological Modulation of Fear Conditioning

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

CB1 antagonist AM251 (1 mg/kg; Tocris) and TRPV1 antagonist Capsazepine (5 mg/kg; Cayman Chemicals) were prepared on the day of injection in vehicle solution (5% polyethylene glycol, 5% Tween-80, 90% saline), and were injected intraperitoneally at a volume of 1 mg/ml 90 min before fear conditioning (day 3). Doses were chosen to be identical to those used in [28 (link)] in order to maintain consistency across studies.

Huckleberry K.A., Calitri R., Li A.J., Mejdell M., Singh A., Bhutani V., Laine M.A., Nastase A.S., Morena M., Hill M.N, & Shansky R.M. (2023). CB1R blockade unmasks TRPV1-mediated contextual fear generalization in female, but not male rats. Neuropsychopharmacology, 48(10), 1500-1508.

+ Open protocol

+ Expand

L-Carnitine Supplementation in Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Capsaicin and capsazepine were obtained from Cayman Chemical Company, Ann Arbor, MI, USA. Supplements for cell culture were ordered from Life Technologies Invitrogen, Karlsruhe, Germany or Biochrom GmbH, Berlin, Germany. Accutase was purchased from PAA Laboratories, Pasching, Austria. All the other reagents, including L-carnitine, were purchased from Sigma-Aldrich, St. Louis, MO, USA.

Lucius A., Chhatwal S., Valtink M., Reinach P.S., Li A., Pleyer U, & Mergler S. (2023). L-Carnitine Suppresses Transient Receptor Potential Vanilloid Type 1 Activation in Human Corneal Epithelial Cells. International Journal of Molecular Sciences, 24(14), 11815.

+ Open protocol

+ Expand

Cell Culture and Patch-Clamp Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Medium and supplements for cell culture were purchased from Biochrom AG (Berlin, Germany) or GIBCO Invitrogen (Karlsruhe, Germany). All reagents (e.g., Ascorbic acid A4403; Trypan Blue T8154), except those specified below, were purchased from Sigma-Aldrich (Deisenhofen, Germany). Capsazepine was purchased from Cayman Chemical Company (Ann Arbor, Mi, USA). The internal and external solutions for planar patch-clamping were provided by Nanion Company (Munich, Germany). Fura2/AM was purchased from PromoCell (Heidelberg, Germany).

Oronowicz J., Reinhard J., Reinach P.S., Ludwiczak S., Luo H., Omar Ba Salem M.H., Kraemer M.M., Biebermann H., Kakkassery V, & Mergler S. (2020). Ascorbate-induced oxidative stress mediates TRP channel activation and cytotoxicity in human etoposide-sensitive and -resistant retinoblastoma cells. Laboratory Investigation; a Journal of Technical Methods and Pathology, 101(1), 70-88.

+ Open protocol

+ Expand

Endocannabinoid Quantification Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

PEA, d₄-PEA, OEA, d₂-OEA, 2-AG and d₅-2-AG were from Cayman Chemical while AEA and d₄-AEA were from R&D systems. [¹⁴C]AEA, rimonabant and SR144528 were obtained from the Drug Supply Program at the National Institute on Drug Abuse. GW6471 was purchased from Sigma while capsazepine was purchased from Cayman Chemical.

Kaczocha M., Glaser S.T., Maher T., Clavin B., Hamilton J., O’Rourke J., Rebecchi M., Puopolo M., Owada Y, & Thanos P.K. (2015). Fatty acid binding protein deletion suppresses inflammatory pain through endocannabinoid/N-acylethanolamine-dependent mechanisms. Molecular Pain, 11, 52.

+ Open protocol

+ Expand

Pharmacological Modulation of TRPV1/TRPV4 in RGCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The TRPV4 agonist GSK1016790A (GSK101) and antagonist HC-067047 (HC-06) were purchased from Sigma. The TRPV1 agonist capsaicin (CAP; 8-methyl-N-vanillyl-6-nonenamide) and the TRPV1 antagonist capsazepine (CPZ; N-[2-(4-Chlorophenyl)ethyl]-1,3,4,5-tetrahydro-7,8-dihydroxy-2H-2-benzazepine-2-carbothioamide) and the endogenous agonist of CB1 receptors 2-arachidonoylglycerol (2-AG) were obtained from Cayman Chemicals. BDNF and CNTF used to culture RGCs were obtained from GenWay Biotech. Other salts and reagents were purchased from Sigma, VWR, Across Organics, or Thermo Fisher. GSK101 (1 mM), HC-06 (10 mM), CAP (10 mM), and CPZ (20 mM) stocks in DMSO were diluted in extracellular saline before use and placed into reservoirs connected to gravity-fed perfusion systems (Warner Instruments).

Lakk M., Young D., Baumann J.M., Jo A.O., Hu H, & Križaj D. (2018). Polymodal TRPV1 and TRPV4 Sensors Colocalize but Do Not Functionally Interact in a Subpopulation of Mouse Retinal Ganglion Cells. Frontiers in Cellular Neuroscience, 12, 353.

+ Open protocol

+ Expand

TRPV1 Inhibition and Intranasal siRNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Capsazepine (Cayman, Ann Arbor, MI, USA) was given 50 µg once daily for 3 months by intraperitoneal injection starting on day 38. TRPV1 siRNA (Bioneer, Daejeon, Korea) was also administrated intranasally 50 µg 2 times per week once per day beginning on day 38 for 3 months, during OVA challenge. The control mice were treated identically with normal saline.

Choi J.Y., Lee H.Y., Hur J., Kim K.H., Kang J.Y., Rhee C.K, & Lee S.Y. (2018). TRPV1 Blocking Alleviates Airway Inflammation and Remodeling in a Chronic Asthma Murine Model. Allergy, Asthma & Immunology Research, 10(3), 216-224.

+ Open protocol

+ Expand

Capsaicin-Induced CREB Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Drugs were applied as described in the figures. capsaicin (10 µm, ApexBio or Cayman Chemical) was applied for 10 min or 24 h. Inhibitors (capsazepine, Cayman Chemical; EGTA, Sigma; H89, Sigma) were pretreated for 10–30 min before capsaicin treatment. All inhibitors were present during capsaicin treatment. H89 was also present during the rest of the 24-h activation phase. All drugs were removed immediately before replating. For phospho-CREB immunostaining, capsaicin was removed 20 min before fixation. For in vivo experiments, surgifoam was dissolved in 200 µm capsaicin or DMSO and applied to the sciatic nerve.

Frey E., Karney-Grobe S., Krolak T., Milbrandt J, & DiAntonio A. (2018). TRPV1 Agonist, Capsaicin, Induces Axon Outgrowth after Injury via Ca2+/PKA Signaling. eNeuro, 5(3), ENEURO.0095-18.2018.

+ Open protocol

+ Expand

Histamine and TRP Receptor Pharmacology

Check if the same lab product or an alternative is used in the 5 most similar protocols

Histamine dihydrochloride and capsaicin (TRPV1 agonist) were purchased from Sigma-Aldrich (Steinheim, Germany). The 4-methylHistamine dihydrochloride (4-MH; H2R /H4R agonist), and histamine trifluoromethyl toluidine dimaleate (HTMT; H1R/H2R agonist) were obtained from Tocris Bioscience (Bristol, UK). ST-1006 (H4R agonist) and JNJ-7777120 (H4R antagonist) were synthesized and provided by Prof. Dr. H. Stark (Heinrich-Heine-Universität, Düsseldorf, Germany) [13 (link),14 (link)]. Allylisothiocyanate (AITC; TRPA1 agonist) and ruthenium red (TRP channel inhibitor) were purchased from Acros Organics, Morris Plaines, NJ, USA. Capsazepine (TRPV1 inhibitor), SB366791 (TRPV1 inhibitor) and HC-030031 (TRPA1 inhibitor) were obtained from Cayman Chemicals (Ann Arbor, MI, USA). Diphenhydramine hydrochloride (H1R antagonist) was purchased from West Ward Pharmaceuticals (Eatontown, NJ, USA).

Wilzopolski J., Kietzmann M., Mishra S.K., Stark H., Bäumer W, & Rossbach K. (2021). TRPV1 and TRPA1 Channels Are Both Involved Downstream of Histamine-Induced Itch. Biomolecules, 11(8), 1166.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!