LPS (Escherichia coli serotype 055:B5), formyl methionyl leucyl phenylalanine (fMLP), interleukin-8 (IL-8), phorbol ester (PMA), cell chromatography C (Cytochrome C), superoxide dismutase (SOD), Elastase, Hydroxyethylpiperazine Ethylsulfonic Acid (HEPES) and Emo were obtained from Sigma-Aldrich (St Louis, MO, USA). Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) ELISA kits were obtained from R&D Systems (Minneapolis, MN). Anti-Myeloperoxidase (MPO) antibody (abcam, ab65871), MNase, RP-1 antibody (BD 550002), SYTOX Green, and Annexin V-FITC were obtained from eBioscience (San Diego, CA). pHrodo red E. coli (Cat.No.4615), pHrodo green S.aureus (Cat. No. 4620) were obtained from Sartorius (Göttingen, Germany). RPMI 1640, fetal bovine serum (FBS), trypsin, and enzyme-free cell dissociation buffer were purchased from Gibco (Grand Island, NY, USA). Penicillin and streptomycin in saline citrate buffer were from Invitrogen (Carlsbad, CA, USA). Other chemical reagents are of analytical grade.
Hydroxyethylpiperazine ethylsulfonic acid hepes
Manufactured by Merck Group
Sourced in United States
Hydroxyethylpiperazine Ethylsulfonic Acid (HEPES) is a chemical compound used as a buffering agent in various laboratory applications. It is a zwitterionic organic compound with the chemical formula C₈H₁₈N₂O₄S. HEPES is widely used to maintain a stable pH in cell culture media and other biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (3 mentions)
Annexin 5 fitc, by Thermo Fisher Scientific (3 mentions)
Sytox green, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
Enzyme free cell dissociation buffer, by Thermo Fisher Scientific (3 mentions)
Streptomycin in saline citrate buffer, by Thermo Fisher Scientific (2 mentions)
Tumor necrosis factor α tnf α and interleukin 1β il 1β elisa kits, by R&D Systems (2 mentions)
Emodin, by Merck Group (2 mentions)
Lipopolysaccharides (lps), by Merck Group (2 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Anti myeloperoxidase mpo antibody, by Abcam (1 mentions)
3 protocols using hydroxyethylpiperazine ethylsulfonic acid hepes
1
Phagocytosis Assay of Neutrophils
2
Immune Cell Activation and Phagocytosis
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Reagents LPS (Escherichia coli serotype 055:B5), formyl methionyl leucyl phenylalanine (fMLP), interleukin-8 (IL-8), phorbol ester (PMA), cell chromatography C (Cytochrome C), superoxide dismutase (superoxide dismutase, SOD), Elastase, Hydroxyethylpiperazine Ethylsulfonic Acid (HEPES) and Emodin were obtained from Sigma-Aldrich (St Louis, MO, USA). Tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1) ELISA kits were obtained from R&D Systems (Minneapolis, MN). MNase, RP-1 antibody (BD 550002), SYTOX Green, and Annexin V-FITC were obtained from eBioscience (San Diego, CA). pHrodo Red E.coli (Cat.No.4615), pHrodo Green S.aureus (Cat. No. 4620) were obtained from Sartorius (Göttingen, Germany). RPMI 1640, fetal bovine serum (FBS), trypsin, and enzyme-free cell dissociation buffer were purchased from Gibco (Grand Island, NY, USA). Penicillin and streptomycin in saline citrate buffer were from Invitrogen (Carlsbad, CA, USA). Other chemical reagents are of analytical grade.
3
Neutrophil Activation and Dysfunction
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Reagents LPS (Escherichia coli serotype 055:B5), formyl methionyl leucyl phenylalanine (fMLP), interleukin-8 (IL-8), phorbol ester (PMA), cell chromatography C (Cytochrome C), superoxide dismutase (SOD), Elastase, Hydroxyethylpiperazine Ethylsulfonic Acid (HEPES) and Emodin were obtained from Sigma-Aldrich (St Louis, MO, USA). Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) ELISA kits were obtained from R&D Systems (Minneapolis, MN). Anti-Myeloperoxidase(MPO) antibody(abcam, ab65871), MNase, RP-1 antibody (BD 550002), SYTOX Green, and Annexin V-FITC were obtained from eBioscience (San Diego, CA). pHrodo Red E. coli (Cat.No.4615), pHrodo Green S.aureus (Cat. No. 4620) were obtained from Sartorius (Göttingen, Germany). RPMI 1640, fetal bovine serum (FBS), trypsin, and enzyme-free cell dissociation buffer were purchased from Gibco (Grand Island, NY, USA). Penicillin and streptomycin in saline citrate buffer were from Invitrogen (Carlsbad, CA, USA). Other chemical reagents are of analytical grade.
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