Reagents and culture mediums for cell cultures were purchased from MilliporeSigma and Thermo Fisher Scientific. Goat anti-mouse IL-6R polyclonal neutralizing Ab (catalog AF1830) and normal goat IgG (catalog AB-108-C) were purchased from R&D Systems, Bio-Techne. Anti-mouse TLR4/MD2 neutralizing Ab (clone MTS510, catalog 117608) was from BioLegend. STAT3 inhibitor (Stattic) was purchased from Santa Cruz Biotechnology and rmIL-6 was from R&D Systems, Bio-Techne. Abs for flow cytometry were PE anti-mouse p-STAT3 (Tyr705, clone 13A3-1, catalog 651004), PE/Cy7, Pacific blue anti-mouse F4/80 (clone BM8, catalog 123114 and 123124), and PE/Cy7 anti-mouse IL-6R (clone D7715A7, catalog 115814) from BioLegend. Abs for Western blotting included anti-mouse p-STAT3 (Tyr705, catalog 9131) and total STAT3 (catalog 9139) from Cell Signaling Technologies. β-actin Ab (clone AC-15, catalog A5441) from MilliporeSigma. Infrared dye–labeled secondary Abs were from Li-Cor Biosciences. For immunocytochemistry staining and FRET analysis, rabbit anti-mouse CIRP Ab (catalog 10209-2-AP) from ProteinTech was used. Goat anti-mouse CD11b Ab (catalog MBS420973) was from MyBiosource. Fluorescence-labeled secondary Ab Cy3-conjugated donkey anti–rabbit IgG (catalog 711-166-152) and Cy5-conjugated donkey anti–goat IgG (catalog 705-175-147) were from Jackson ImmunoResearch Laboratories.
Anti mouse p stat3 tyr705
Manufactured by Cell Signaling Technology
Anti-mouse p-STAT3 (Tyr705) is a laboratory reagent used to detect the phosphorylated form of STAT3 protein at tyrosine 705 residue in mouse samples. It is designed for use in techniques such as Western blotting and immunohistochemistry to study the activation of STAT3 signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Stat3 inhibitor stattic, by Santa Cruz Biotechnology (2 mentions)
Normal goat igg, by R&D Systems (1 mentions)
Rmil 6, by R&D Systems (1 mentions)
Total stat3, by Cell Signaling Technology (1 mentions)
Infrared dye labeled secondary abs, by LI COR (1 mentions)
Cy5 conjugated donkey anti goat igg, by Jackson ImmunoResearch (1 mentions)
Cy3 conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Opsonizing reagent, by Thermo Fisher Scientific (1 mentions)
Anti p cofilin, by Cell Signaling Technology (1 mentions)
Anti arp2, by Cell Signaling Technology (1 mentions)
Infrared dye labeled secondary antibody, by LI COR (1 mentions)
Anti total stat3, by Cell Signaling Technology (1 mentions)
2 protocols using anti mouse p stat3 tyr705
1
Investigating STAT3 Signaling in Macrophages
2
Phagocytosis Assay with pHrodo Conjugates
Check if the same lab product or an alternative is used in the 5 most similar protocols
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pHrodo E. coli (K12 strain) bioparticle conjugates (catalog no: P35366 for green florescence, and P35361 for red florescence) and an opsonizing reagent (catalog no: E2870) were purchased from Thermo Fisher Scientific (Carlsbad, CA). Cell culture media were purchased from Thermo Fisher Scientific. The antibodies for Western blotting included anti-ARP2 (catalog no: 5614), anti-p-cofilin (catalog no: 3313), anti-total cofilin (catalog no: 5175), anti-βPIX (catalog no: 4515), anti-mouse p-STAT3 (Tyr705, catalog no: 9131), and anti-total STAT3 (catalog no: 9139); these were obtained from Cell Signaling Technologies (Danvers, MA). Anti-β-actin antibodies (clone AC-15, catalog no: A5441), an anti-Rac1 magnetic bead conjugate (catalog no: 16-319), and anti-Rac1 antibodies (catalog no: 05-389) were purchased from Millipore-Sigma (St. Louis, MO). A Rac1 activation magnetic bead pulldown assay kit (catalog no: 17-10394) was purchased from Millipore-Sigma (St. Louis, MO). Infrared dye-labeled secondary antibodies were obtained from Li-Cor Biosciences (Lincoln, NE). The STAT3 inhibitor stattic (catalog no: sc-202818) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). The Rac1 inhibitor NSC 23766 (catalog no: 13196) was purchased from Cayman Chemicals (Ann Arbor, MI). A MemBrite cell surface staining kit was purchased from Biotium (catalog no: 30093-T, Fremont, CA).
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