V plex mouse proinflammatory cytokine panel 1 kit

BMDCs derived from the femurs of two BALB/c mice were isolated and matured based on a study published by Jin et al. [26 (link)]. Detailed descriptions of the maturation conditions, transduction, and staining are provided in [18 (link)]. Twenty-four hours after the transduction of the murine BMDCs, the supernatant was collected. The concentrations of four different proinflammatory biomarkers were determined using a customised V-PLEX mouse proinflammatory cytokine panel 1 kit (K15048D; Mesoscale, Rockville, MD, USA). The supernatants were diluted 1:5, and the samples were assessed in duplicates. The biomarker concentrations were analysed with a MESO QuickPlex SQ 120 MM instrument (Mesoscale, Rockville, MD, USA).

Supernatants from BMDCs were isolated and frozen 24 h post-transduction. Concentrations of different biomarkers in the supernatant were measured in duplicates using a customized version of the V-PLEX mouse proinflammatory cytokine panel 1 kit (K15048D, Mesoscale, Rockville, MD, USA) at a 1:5 dilution, according to the instructions of the kit. Concentrations were analysed using the MESO QuickPlex SQ 120MM (Mesoscale, Rockville, MD, USA).