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Pe rabbit anti active caspase 3

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The PE rabbit anti-active caspase-3 is a laboratory reagent used to detect and quantify activated caspase-3 in biological samples. Caspase-3 is a key enzyme involved in the apoptosis (programmed cell death) pathway. The PE-conjugated antibody specifically binds to the active form of caspase-3, enabling its identification and measurement.

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Lab products found in correlation

Cytochrome c, by Cell Signaling Technology (1 mentions) Cellrox green, by Thermo Fisher Scientific (1 mentions) P h2ax, by Cell Signaling Technology (1 mentions) Fixation permeabilization solution kit, by BD (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) Alexa fluor 700 mouse anti cleaved parp asp214, by BD (1 mentions) Mitoscreen jc 1, by BD (1 mentions) Cleaved caspase 8, by Cell Signaling Technology (1 mentions) Cell counting kit 8 (cck8), by Merck Group (1 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) N acetylcysteine, by Merck Group (1 mentions) Fitc annexin 5 apoptosis detection kit 1, by BD (1 mentions) Apo direct kit, by BD (1 mentions) Cytoflex flow cytometry system, by Beckman Coulter (1 mentions) Cytexpert, by Beckman Coulter (1 mentions) Canto 2 analyzer, by BD (1 mentions) Cell death detection elisa kit, by Roche (1 mentions)

3 protocols using pe rabbit anti active caspase 3

1

Multiparametric Apoptosis Assessment

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BTZ, Cell Counting Kit-8, and N-acetylcysteine (NAC) were purchased from Sigma Aldrich (St. Louis, MO, USA). z-VAD-FMK was purchased from Calbiochem (San Diego, CA, USA). Antibodies against Skp2, p21, p27, MTH1, CDK4, CDK6, caspase-9, Bcl-2, cleaved caspase-3, caspase-3, Bax, p53, Cytochrome c, PARP, Cleaved caspase-8, PH2AX were purchased from Cell Signaling Technology (Danvers, MA, USA). FITC Annexin V apoptosis detection kit I, Apo-Direct kit, Fixation/Permeabilization solution kit, BD MitoScreen (JC1), BV421 mouse anti-γH2AX (pS139), PE rabbit anti-active caspase-3, and Alexa Fluor 700 mouse anti-cleaved PARP (Asp214) antibodies were purchased from BD Biosciences (San Jose, USA). CellROXGreen was purchased from Invitrogen (Massachusetts, USA).
Prabhu K.S., Ahmad F., Kuttikrishnan S., Leo R., Ali T.A., Izadi M., Mateo J.M., Alam M., Ahmad A., Al-Shabeeb Akil A.S., Bhat A.A., Buddenkotte J., Pourkarimi E., Steinhoff M, & Uddin S. (2024). Bortezomib exerts its anti-cancer activity through the regulation of Skp2/p53 axis in non-melanoma skin cancer cells and C. elegans. Cell Death Discovery, 10, 225.
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2

Apoptosis Detection via Flow Cytometry

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The experimental process followed a previous study with modifications.28 (link) At 24 and 48 hours after irradiation, the cells were harvested and washed twice with 4 °C PBS and stained with PE rabbit anti-active caspase-3 (BD Biosciences). Then, 10,000 events were collected by the CytoFlex flow cytometry system (Beckman). Data were further analyzed by CytExpert (Beckman).
Huang C.Y., Lai Z.Y., Hsu T.J., Chou F.I., Liu H.M, & Chuang Y.J. (2022). Boron Neutron Capture Therapy Eliminates Radioresistant Liver Cancer Cells by Targeting DNA Damage and Repair Responses. Journal of Hepatocellular Carcinoma, 9, 1385-1401.
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3

Apoptosis Assays for Cell Lines

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Cells (1.5×104) were seeded into 6-well plates for 24 hr before incubation with MCF10A full medium, MEBM added BPE (Lonza, Allendale, NJ), or DMEM medium without glucose and L-glutamine for another 24 hr. Apoptosis-induced DNA fragmentation was measured using the Cell Death Detection ELISA Kit (Roche Applied Science, Indianapolis, IN) according to manufacturer's instructions. Apoptosis-induced the active form of caspase-3 was tested using the PE Rabbit Anti-Active Caspase-3 (BD Biosciences, San Jose, CA) according to manufacturer's instructions. BD Canto II analyzer is used to read active caspase-3 in the PE channel.
Han L., Diao L., Yu S., Xu X., Li J., Zhang R., Yang Y., Werner H.M., Eterovic A.K., Yuan Y., Li J., Nair N., Minelli R., Tsang Y.H., Cheung L.W., Jeong K.J., Roszik J., Ju Z., Woodman S.E., Lu Y., Scott K.L., Li J.B., Mills G.B, & Liang H. (2015). The Genomic Landscape and Clinical Relevance of A-to-I RNA Editing in Human Cancers. Cancer cell, 28(4), 515-528.
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