Cd68 antibody

Growth factor-reduced Matrigel matrix was purchased from Corning (Bedford, MA, USA). FxCycle™ PI/RNase Staining Solution, H2DCFDA, DAF-FM diacetate, and DAPI were bought from Life Technologies Corporation (Eugene, OR, USA). Anhydrous sodium sulfide (Na₂S), Pierce BCA protein assay kit, RIPA buffer, protease and phosphatase inhibitor mini tablets, RevertAid RT Reverse Transcription Kit, PowerUp SYBR™ Green Master Mix, TRIzol Reagent and CD68 antibody were procured from Thermo Scientific (Rockford, IL, USA). Na₂S solution was prepared in molecular biology grade water. Human oxidized low-density lipoprotein (oxLDL) was acquired from Kalen Biomedical, LLC (Montgomery, MD, USA). Cell proliferation reagent WST-1 was obtained from Roche Diagnostics GmbH (Mannheim, Germany). Phospho-eNOS (Ser1177), phospho-AKT (Ser473), phospho-ERK1/2 (Thr202/Tyr204), total AKT, total ERK1/2, total eNOS, Ki67, and CD45 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). GAPDH antibody was procured from Santa Cruz Biotechnology (Dallas, TX, USA). LYVE-1 antibody was purchased from Abcam (Cambridge, MA, USA). CD31 antibody was obtained from R&D Systems, Inc. (Minneapolis, MN, USA). Ready-to-use intercept blocking buffer to block the nitrocellulose membranes was purchased from Li-Cor Biosciences (Lincoln, NE, USA).

Raw264.7 (mouse macrophage) was obtained from Fuheng Cell Bank, Fudan University (Shanghai, China) for the in vitro study. High glucose (4,500 mg^–1 mL) Dulbecco′s Modified Eagle′s Medium (DMEM), penicillin/streptomycin and fetal bovine serum were purchased from Gibco (Carlsbad, CA, United States). The CD68 antibody and corresponding 2nd antibody were purchased from Thermo Fisher Scientific (United States). Cell Counting Kit-8 (CCK-8) and Calcein-AM/PI Double Stain Kit were purchased from Thermo Fisher Scientific (United States).