Donkey serum

Manufactured by Cedarlane

Sourced in United States

Donkey serum is a biological product derived from the blood of donkeys. It is a complex mixture of proteins, antibodies, and other biomolecules. The core function of donkey serum is to serve as a versatile component in various research and diagnostic applications.

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Lab products found in correlation

Goat serum, by Cedarlane (2 mentions) Myogenin, by Santa Cruz Biotechnology (1 mentions) Mouse anti biotin, by Jackson ImmunoResearch (1 mentions) Myod c 20, by Santa Cruz Biotechnology (1 mentions) C ebpβ, by Abcam (1 mentions) Alexa flour 647, by Jackson ImmunoResearch (1 mentions) Cyanine3 (cy3), by Jackson ImmunoResearch (1 mentions) Alexa 488, by Jackson ImmunoResearch (1 mentions)

2 protocols using donkey serum

Immunofluorescence Staining of Myogenic Cells

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Myofibers were fixed in 4% paraformaldehyde (PFA) in PBS and 1% glycine and blocked in PBS containing 0.2% Triton X-100 (BioShop), 2% BSA, 5% goat serum (Cedarlane), and 1% azide. Myoblasts were fixed in 2% PFA in PBS and blocked in PBS containing 0.3% Triton X-100 and 10% goat serum. Cryosections were thawed at room temperature, fixed in 4% PFA, and processed for antigen retrieval in citrate buffer at 95 °C for 20 min. The sections were permeabilized with PBS containing 0.5% Triton X-100 and blocked in PBS containing 0.1% Triton X-100 and 5% donkey serum (Cedarlane) prior to incubation with primary antibody overnight at 4 °C. The cells were washed with PBS and incubated in biotin anti-mouse (when indicated) or secondary antibodies conjugated to a fluorescent dye (Cy3, Alexa 488, or Alexa Flour 647; all from Jackson ImmunoResearch). Nuclei were counterstained with DAPI (0.5 μg/ml). The primary antibodies used were as follows: Pax7-c (DSHB), MYH (H-300; Santa Cruz), MyoD (C-20; Santa Cruz), myogenin (M-225; Santa Cruz), C/EBPβ (E299; Abcam), and laminin (AL-4; Millipore).

Lala-Tabbert N., AlSudais H., Marchildon F., Fu D, & Wiper-Bergeron N. (2016). CCAAT/enhancer binding protein β is required for satellite cell self-renewal. Skeletal Muscle, 6, 40.

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Corneal Tissue Immunostaining Protocol

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Corneas were dissected and fixed at room temperature for 1 hour in 1.3% paraformaldehyde in PBS, and radial incisions were made to facilitate flat-mounting of the corneal tissues. Corneas were washed in PBS five times, permeabilized in 1% Triton-X100 in PBS at room temperature for 60 minutes and blocked with either 20% goat serum or donkey serum (Cedarlane, Burlington, NC, USA) in blocking buffer (0.3% Triton-X-100/0.1% Tween-20 in PBS) for 1 hour. The corneas were then incubated in a 100μl cocktail of primary antibodies at room temperature for 2 hours, followed by an additional incubation overnight at 4 °C. After five 5-minute washes in wash buffer (0.1% Tween-20 in PBS), the corneas were incubated in a 100μl cocktail of secondary antibodies in blocking buffer at room temperature for 2 hours. Following five 10-minute washes with wash buffer, the corneas were mounted on slides and dried at 4°C for at least 12 hours before imaging.

Yun H., Yee M.B., Lathrop K.L., Kinchington P.R., Hendricks R.L, & St. Leger A.J. (2020). Production of the cytokine VEGF-A by infiltrating CD4+ T and myeloid cells disrupts the corneal nerve landscape and promotes herpes stromal keratitis. Immunity, 53(5), 1050-1062.e5.

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