Mab401

Manufactured by R&D Systems

MAB401 is a Mouse Monoclonal Antibody. It is targeted against Human Activin A.

Automatically generated - may contain errors

Lab products found in correlation

Baf401, by R&D Systems (2 mentions) Mouse cxcl1 kc duoset elisa, by R&D Systems (1 mentions) Mab453, by R&D Systems (1 mentions) Mab275, by R&D Systems (1 mentions) Baf453, by R&D Systems (1 mentions) Ebio17ck15a5, by Thermo Fisher Scientific (1 mentions) Baf275, by R&D Systems (1 mentions) Quantikine elisa kit, by R&D Systems (1 mentions) Luminex multiplex assay, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

3 protocols using mab401

Quantifying Cytokines and Metabolites in Mouse Brain

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BMDM supernatants were assayed for IL-1β and KC by ELISA. Antibody pairs for the IL-1β (MAB401 and BAF401; clone 30311 and polyclonal) ELISA were purchased from R&D Systems. The KC ELISA was performed using the Mouse CXCL1/KC DuoSet ELISA (R&D Systems, Cat. No. DY453) according to the manufacturer’s instructions.
For the BHB ELISA, 10 mg of cortex was removed from the brains of mice. The tissue was disrupted, and the proteins were precipitated with perchloric acid. The samples were centrifuged, and the cleared supernatants were analyzed using the colorimetric β-hydroxybutyrate Assay Kit (Abcam, Cat. No. ab83390) according to the manufacturer’s instructions.
Supernatants from mouse brain cortex homogenates were assayed for IL-1β by ELISA as described above. The supernatants were prepared by homogenizing 2.5 mg of cortex in 0.25 ml of sterile PBS. Cells and debris were removed by centrifugation, and supernatants were collected for analysis.

Shippy D.C., Wilhelm C., Viharkumar P.A., Raife T.J, & Ulland T.K. (2020). β-Hydroxybutyrate inhibits inflammasome activation to attenuate Alzheimer’s disease pathology. Journal of Neuroinflammation, 17, 280.

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Cytokine Profiling of Respiratory Samples

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Culture supernatants and BAL samples were assayed for IL-1β, MIP-1α, TNFα, IL-6, IL-10, IL-18, IL-17A and CXCL1 by ELISA. Antibody pairs for the IL-1β (MAB401 and BAF401; clone 30311 and polyclonal; R&D Systems; 8 and 4 μg ml⁻¹, respectively) and CXCL1 (mouse: MAB453 and BAF453; clone 48415 and polyclonal; R&D Systems; 8 and 0.4 μg ml⁻¹, respectively; human: MAB275, clone 20326, 4 μg ml⁻¹; BAF275, polyclonal, 40 ng ml⁻¹) and CCL3/ MIP-1α (Quantikine ELISA kit, Product #MMA00) ELISAs were from R&D Systems. Antibody pairs for IL-1α (capture: product #14-7011-85, clone ALF-161; detection: product #13-7111-85, polyclonal), TNFα (capture: product #14-7325-85, clone 1F3F3D4; detection: product #13-7326-85, clones MP6-XT22 and MP6-XT3), IL-6 (capture: product #14-7061-85, clone MP5-20F3; detection: product #13-7062-85, clone MP5-32C11), IL-10 (capture: product #14-7101-85, clone JES5-16E3; detection: product #13-7102-85, clone JES5-2A5) and IL-17A (capture: product #14-7175-85, clone eBio 17CK15A5; detection: product #13-7177-85, clone eBio17B7) were from eBiosciences and used at a 1:250 dilution. Antibody pairs for IL-18 were from MBL International (capture: product #D047-3, clone 74, 1:1,000; detection: product #D048-6, clone 93-10C, 1:2,000).

Ulland T.K., Jain N., Hornick E.E., Elliott E.I., Clay G.M., Sadler J.J., Mills K.A., Janowski A.M., Volk A.P., Wang K., Legge K.L., Gakhar L., Bourdi M., Ferguson P.J., Wilson M.E., Cassel S.L, & Sutterwala F.S. (2016). Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment. Nature Communications, 7, 13180.

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Cytokine Profiling of Leishmania-Infected Mice

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Single cell suspensions were generated from draining retromaxillary lymph nodes or spleens from L. major infected or control mice by passage through a sterile 70 µM screen. Red blood cells in splenic preparations were lysed by hypotonic shock. The cells were then seeded at 2×10⁵ cells/well in 200 µl of RP10 [RPMI 1640 (Gibco), 10% heat-inactivated FBS (Gibco), 2 mM L-glutamine, 50 µg/ml gentamicin, in round bottomed 96-well plates with or without stimuli. The stimuli included media control, 10 µg/ml total Leishmania lysate, live L. major parasites at 3:1 parasite: host cell ratio, or wells coated with anti-CD3e antibody at 500 ng/mL overnight. After incubation at 37°, 5% CO₂ for 72 hours, supernatants were collected for cytokine assays. The murine cytokines IFN-γ, IL-1β, IL-4, IL-12p70, IL-6, IL-17A, TNFα, and IL-10 were analyzed in cell supernatants by a Multiplex Luminex Assay (Life Technologies) or Bio-Plex Multiplex Assay (Millipore). IL-17A was detected in whole ear lysates by ELISA using the mouse IL-17A DuoSet ELISA kit from R&D. IL-1β was detected in by ELISA using monoclonal coating and detection antibodies MAB401 and BAF401, respectively, from R&D.

Clay G.M., Valadares D.G., Graff J.W., Ulland T.K., Davis R.E., Scorza B.M., Zhanbolat B.S., Chen Y., Sutterwala F.S, & Wilson M.E. (2017). An anti-inflammatory role for NLRP10 in murine cutaneous leishmaniasis. Journal of immunology (Baltimore, Md. : 1950), 199(8), 2823-2833.

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