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Anti γ tubulin

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Anti γ-tubulin is an antibody that specifically binds to the γ-tubulin protein, which is a key component of the microtubule organizing center (MTOC) in eukaryotic cells. This antibody can be used to detect and localize γ-tubulin in various cell types and tissues.

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Lab products found in correlation

Cantharidin, by Merck Group (2 mentions) Doxycycline, by Merck Group (2 mentions) Crystal violet, by Merck Group (2 mentions) Cyclin b, by Cell Signaling Technology (2 mentions) Phospho plk1 antibodies, by BioLegend (2 mentions) Anti bubr1 antibody, by BD (2 mentions) Secondary fit c and trit c antibodies, by Jackson ImmunoResearch (2 mentions) Secondary alexa 488, by BD (2 mentions) Pbabe puro ires gfp, by Addgene (2 mentions) Anti α tubulin, by Merck Group (2 mentions) Anti cyclin b, by Santa Cruz Biotechnology (1 mentions) Anti e cadherin antibody, by BD (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti vimentin antibody, by Cell Signaling Technology (1 mentions) Anti phospho s10 h3, by Cell Signaling Technology (1 mentions) Anti β tubulin, by Merck Group (1 mentions) Anti gfp, by Santa Cruz Biotechnology (1 mentions) Anti α tubulin, by Abcam (1 mentions)

3 protocols using anti γ tubulin

1

Antibody Characterization for TAZ Signaling

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The TAZ (V386) antibody from Cell Signaling Technology was used for Western blotting throughout the study. Rabbit polyclonal phospho-specific antibodies against TAZ S90, S105, T326, and T346 were generated and purified by AbMart. Anti-β-actin, anti-GFP, and anti-cyclin B antibodies were from Santa Cruz Biotechnology. Anti-glutathione S-transferase (GST) and anti-His antibodies were from Bethyl Laboratories. Anti-phospho-S10 H3 and anti-vimentin antibodies were from Cell Signaling Technology. Anti-E-cadherin antibody was from BD Biosciences. Anti-β-tubulin (Sigma), anti-α-tubulin (Abcam), and anti-γ-tubulin (Biolegend) antibodies were used for immunofluorescence staining.
Zhang L., Chen X., Stauffer S., Yang S., Chen Y, & Dong J. (2015). CDK1 phosphorylation of TAZ in mitosis inhibits its oncogenic activity. Oncotarget, 6(31), 31399-31412.
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2

Molecular Tools for Cell Cycle Research

Cited 1 time
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Anti α-tubulin, doxycycline, crystal violet and cantharidin were purchased from Sigma. Anti γ-tubulin and phospho Plk1 antibodies were purchased from Biolegend. Antibodies against pan phospho aurora kinase, HA, phospho histone3 (Ser 10) and cyclin B were purchased from Cell Signaling Technology. Anti-BubR1 antibody was purchased from BD Biosciences, and centromere autoantibodies were purchased from Immunovision. Secondary FIT-C and TRIT-C antibodies were obtained from Jackson Immunoresearch Laboratories. Secondary Alexa 488 and Alexa 568 were obtained from BD Biosciences. Aphidicolin, hydroxyurea, etoposide and okadaic acid were obtained from Calbiochem. pTREX plasmid was a gift from Novartis Pharmaceuticals. PyST was cloned in the pTREX plasmid using standard molecular biology techniques. Dominant negative p53 (pWZL-p53DD) was a gift from Dr. Jean Zhao. ST-HA-FLAG was also cloned in pBABE-puro-IRES-GFP (obtained from Addgene # 14430). PyST-Cdt1 and Geminin degron constructs were cloned by swapping PyST for SV40ST in the MSCV-puro-SV40-Cdt1/Geminin plasmids generated in Dr. David Livingston's lab. pRXTN50 (link) plasmid was a gift from Dr. James Iglehart.
Fernando A.T., Andrabi S., Cizmecioglu O., Zhu C., Livingston D.M., Higgins J.M., Schaffhausen B.S, & Roberts T.M. (2014). Polyoma small T antigen triggers cell death via mitotic catastrophe. Oncogene, 34(19), 2483-2492.
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3

Molecular Tools for Cell Cycle Research

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti α-tubulin, doxycycline, crystal violet and cantharidin were purchased from Sigma. Anti γ-tubulin and phospho Plk1 antibodies were purchased from Biolegend. Antibodies against pan phospho aurora kinase, HA, phospho histone3 (Ser 10) and cyclin B were purchased from Cell Signaling Technology. Anti-BubR1 antibody was purchased from BD Biosciences, and centromere autoantibodies were purchased from Immunovision. Secondary FIT-C and TRIT-C antibodies were obtained from Jackson Immunoresearch Laboratories. Secondary Alexa 488 and Alexa 568 were obtained from BD Biosciences. Aphidicolin, hydroxyurea, etoposide and okadaic acid were obtained from Calbiochem. pTREX plasmid was a gift from Novartis Pharmaceuticals. PyST was cloned in the pTREX plasmid using standard molecular biology techniques. Dominant negative p53 (pWZL-p53DD) was a gift from Dr. Jean Zhao. ST-HA-FLAG was also cloned in pBABE-puro-IRES-GFP (obtained from Addgene # 14430). PyST-Cdt1 and Geminin degron constructs were cloned by swapping PyST for SV40ST in the MSCV-puro-SV40-Cdt1/Geminin plasmids generated in Dr. David Livingston's lab. pRXTN50 (link) plasmid was a gift from Dr. James Iglehart.
Fernando A.T., Andrabi S., Cizmecioglu O., Zhu C., Livingston D.M., Higgins J.M., Schaffhausen B.S, & Roberts T.M. (2014). Polyoma small T antigen triggers cell death via mitotic catastrophe. Oncogene, 34(19), 2483-2492.
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