All foods and food products were purchased from local markets (Davis and Sacramento, CA, USA) including Safeway, Trader Joe’s, Davis Food Co-op, Whole Foods, Nugget Markets, Target, and online (Amazon). Trifluoroacetic acid (TFA, HPLC grade), 3-methyl-1-phenyl-2-pyrazoline-5-one (PMP), chloroform (HPLC grade), ammonium hydroxide solution (NH4OH) (28–30%), ammonium acetate, sodium acetate, glacial acetic acid, methanol (HPLC grade), D-fructose, D-mannose, D-allose, D-glucose, D-galactose, L-rhamnose, L-fucose, D-ribose, D-xylose, L-arabinose, N-acetyl-D-glucosamine (GlcNAc), N-acetyl-D-galactosamine (GalNAc), D-glucuronic acid (GlcA), and D-galacturonic acid (GalA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Arabinoxylan and polygalacturonic acid were purchased from Megazyme (Bray, Ireland). 96-well Nunc plates and lids were purchased from Thermo Scientific. Viscozyme was provided by Novozyme (Davis, CA, USA). Acetonitrile (ACN) (HPLC grade) was purchased from Honeywell (Muskegon, MI, USA). Nanopure water was used for all experiments.
D galacturonic acid gala
Manufactured by Merck Group
Sourced in United States
D-galacturonic acid (GalA) is a chemical compound commonly used in laboratory settings. It is a type of uronic acid, which are carbohydrate derivatives. GalA can be utilized as a standard or reference material in various analytical procedures and assays.
Automatically generated - may contain errors
Lab products found in correlation
L fucose, by Merck Group (2 mentions)
D allose, by Merck Group (2 mentions)
L rhamnose, by Merck Group (2 mentions)
D xylose, by Merck Group (2 mentions)
D mannose, by Merck Group (2 mentions)
D glucuronic acid glca, by Merck Group (2 mentions)
D galactose, by Merck Group (2 mentions)
N acetyl d galactosamine galnac, by Merck Group (2 mentions)
L arabinose, by Merck Group (2 mentions)
N acetyl d glucosamine glcnac, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
D fructose, by Merck Group (2 mentions)
Glacial acetic acid, by Merck Group (2 mentions)
D glucose, by Merck Group (2 mentions)
Sodium acetate, by Merck Group (2 mentions)
D ribose, by Merck Group (1 mentions)
Hplc grade acetonitrile acn, by Honeywell (1 mentions)
Arabinoxylan, by Megazyme (1 mentions)
Polygalacturonic acid, by Megazyme (1 mentions)
Anhydrous dimethyl sulfoxide, by Merck Group (1 mentions)
3 protocols using d galacturonic acid gala
1
Comprehensive Carbohydrate Analysis Protocol
2
Comprehensive Glycan Analysis Protocol
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Sodium acetate, hydrogen peroxide (H2O2) (30% v/v), iron(III)
sulfate pentahydrate (Fe2(SO4)3), trifluoroacetic acid
(TFA) (HPLC grade), chloroform (HPLC grade), ammonium acetate, sodium hydroxide pellets
(semiconductor grade 99.99% trace metals basis), ammonium hydroxide solution
(NH4OH) (28–30%), 3-methyl-1-phenyl-2-pyrazoline-5-one (PMP),
dichloromethane (DCM), methanol (HPLC grade), anhydrous dimethyl sulfoxide (DMSO), glacial
acetic acid,d -galactose, d -mannose, d -glucose,
d -allose, d -fructose, d -xylose, l -arabinose,
d -xylose, l -fucose, l -rhamnose,
N-acetyl-d -glucosamine (GlcNAc),
N-acetyl-d -galactosamine (GalNAc), d -glucuronic acid
(GlcA), andd -galacturonic acid (GalA) were purchased from Sigma-Aldrich (St.
Louis, MO). Maltotriose, maltotetraose, maltopentaose, and maltohexaose were obtained from
Carbosynth (Compton, UK). Galactan was purchased from Megazyme (Bray, Ireland). C18 and
porous graphitized carbon (PGC) solid phase extraction (SPE) plates were purchased from
Glygen (Columbia, MD). Formic acid (FA) (99.5% optima LC/MS grade) was purchased from
Fisher Scientific (Hampton, NH). Acetonitrile (ACN) (HPLC grade) was purchased from
Honeywell (Muskegon, MI). All purchased chemicals were used without further purification.
Butternut squash was purchased from Pedrick Produce (Dixon, CA). Nanopure water was used
for all experiments.
sulfate pentahydrate (Fe2(SO4)3), trifluoroacetic acid
(TFA) (HPLC grade), chloroform (HPLC grade), ammonium acetate, sodium hydroxide pellets
(semiconductor grade 99.99% trace metals basis), ammonium hydroxide solution
(NH4OH) (28–30%), 3-methyl-1-phenyl-2-pyrazoline-5-one (PMP),
dichloromethane (DCM), methanol (HPLC grade), anhydrous dimethyl sulfoxide (DMSO), glacial
acetic acid,
N-acetyl-
N-acetyl-
(GlcA), and
Louis, MO). Maltotriose, maltotetraose, maltopentaose, and maltohexaose were obtained from
Carbosynth (Compton, UK). Galactan was purchased from Megazyme (Bray, Ireland). C18 and
porous graphitized carbon (PGC) solid phase extraction (SPE) plates were purchased from
Glygen (Columbia, MD). Formic acid (FA) (99.5% optima LC/MS grade) was purchased from
Fisher Scientific (Hampton, NH). Acetonitrile (ACN) (HPLC grade) was purchased from
Honeywell (Muskegon, MI). All purchased chemicals were used without further purification.
Butternut squash was purchased from Pedrick Produce (Dixon, CA). Nanopure water was used
for all experiments.
3
Detailed Characterization of Dendrobium Rhizome
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A
PL aquagel-OH MIXED-H column (7.5 mm × 300 mm, 8 μm) was
bought from Agilent (Santa Clara, CA, USA). Sulfur was purchased from
Sigma-Aldrich (Steinheim, Germany). Paraffin wax was bought from Leica
Biosystem (Richmond, USA). Methanol and acetonitrile were of MS-grade
and purchased from Merck Co., Ltd. (Darmstadt, Germany). Formic acid
(MS-grade) was provided by Sigma-Aldrich (Steinheim, Germany). Trifluoroacetic
acid (TFA) (99%) was purchased from International Laboratory USA (South
San Francisco, CA, USA). Ultrapure water was produced by a Milli-Q
water purification system (Milford, MA, USA). Reference standards
with purity ≥98%, includingd -glucose (Glc), d -galactose (Gal), d -galacturonic acid (GalA), and a series
of dextran standards (MW 2000, 670, 410, 270, 150, 80, 50, 12, 5,
and 1 kDa), were purchased from Sigma (St. Louis, MO, USA). Other
solvents and chemicals were of analytical grade.
Fresh DR samples
were collected from Henan province, China. Twenty batches of commercial
DR samples were purchased in Hong Kong and mainland China (Table S1 ). All collected samples were authenticated
by Dr. Jun Xu. Voucher specimens of the DR samples were deposited
at the School of Chinese Medicine, Hong Kong Baptist University.
PL aquagel-OH MIXED-H column (7.5 mm × 300 mm, 8 μm) was
bought from Agilent (Santa Clara, CA, USA). Sulfur was purchased from
Sigma-Aldrich (Steinheim, Germany). Paraffin wax was bought from Leica
Biosystem (Richmond, USA). Methanol and acetonitrile were of MS-grade
and purchased from Merck Co., Ltd. (Darmstadt, Germany). Formic acid
(MS-grade) was provided by Sigma-Aldrich (Steinheim, Germany). Trifluoroacetic
acid (TFA) (99%) was purchased from International Laboratory USA (South
San Francisco, CA, USA). Ultrapure water was produced by a Milli-Q
water purification system (Milford, MA, USA). Reference standards
with purity ≥98%, including
of dextran standards (MW 2000, 670, 410, 270, 150, 80, 50, 12, 5,
and 1 kDa), were purchased from Sigma (St. Louis, MO, USA). Other
solvents and chemicals were of analytical grade.
Fresh DR samples
were collected from Henan province, China. Twenty batches of commercial
DR samples were purchased in Hong Kong and mainland China (
by Dr. Jun Xu. Voucher specimens of the DR samples were deposited
at the School of Chinese Medicine, Hong Kong Baptist University.
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