Byk204165

3T3-L1 cells were grown until confluent and exposed to various treatments for the experiments described herein. For experiments with PARP inhibitor, the cells were treated with 20 μM BYK204165 (Tocris Bioscience, 1104546–89-5), or DMSO vehicle for 2 hours. For activation of AMPKα, the cells were treated with 500 μM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR, Sigma, A9978) for 1 hour. For inhibition of AMPKα, the cells were treated with 20 μM Dorsomorphin (Sigma, P5499) for 1 hour. For inhibition of phosphatase, the cells were treated with 1 μM Okadaic acid (Sigma, 459620) for 15 minutes. For inhibition of Ca²⁺/Calmodulin-dependent protein kinase kinase (CaMKK), the cells were treated with 10 μg/mL STO-609 (Sigma, S1318) for 1 hour. For treatment with 2-deoxy-D-glucose (5 mM; Sigma, D8375), the cells were grown until confluent and treated for 24 hours. For induction of DNA damage, the cells were treated with 2 mM H₂O₂ for 5 minutes.