Log In Sign up
The largest database of trusted experimental protocols

Verikine mouse interferon alpha elisa kit

Manufactured by PBL Assay Science
Visit Supplier
Sourced in United States

The VeriKine Mouse Interferon Alpha ELISA Kit is a laboratory assay used to measure the levels of interferon alpha in mouse samples. It is a quantitative enzyme-linked immunosorbent assay (ELISA) designed to detect and quantify the presence of this cytokine.

Automatically generated - may contain errors

Lab products found in correlation

Cba enhanced sensitivity flex set, by BD (2 mentions) Verikine human ifn alpha multi subtype elisa kit, by PBL Assay Science (2 mentions) Quantikine elisa kit, by R&D Systems (2 mentions) Elisa plate, by Greiner (2 mentions) Mouse tnf α elisa ready set go, by Thermo Fisher Scientific (1 mentions) Mouse il 12p40 elisa ready set go, by Thermo Fisher Scientific (1 mentions) Mouse il 6 elisa ready set go, by Thermo Fisher Scientific (1 mentions) Bio plex pro mouse cytokine 23 plex immunoassay, by Bio-Rad (1 mentions) Verikine mouse interferon beta elisa kit, by PBL Assay Science (1 mentions) Bio plex protm mouse cytokine 23 plex assay, by Bio-Rad (1 mentions) Infinite m1000 plate reader, by Tecan (1 mentions)

16 protocols using verikine mouse interferon alpha elisa kit

1

Multiplex Immunoassay of Cytokines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tnf-α, Cxcl1 and Il-6 levels in mouse plasma samples were measured using the ProcartaPlexTM Multiplex Immunoassay technology from Affymetrix (Affymetrix, eBioscience), according to the manufacturer’s protocol. Ifn-α levels from pDC culture supernatants were analyzed according to the manufacturer’s protocol with a VeriKine mouse Interferon Alpha ELISA Kit (pbl Assay Science).
Mandl M., Drechsler M., Jansen Y., Neideck C., Noels H., Faussner A., Soehnlein O., Weber C, & Döring Y. (2015). Evaluation of the BDCA2-DTR Transgenic Mouse Model in Chronic and Acute Inflammation. PLoS ONE, 10(8), e0134176.
+ Open protocol
+ Expand
2

Serum Interferon-Alpha Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum interferon-alpha levels were determined by ELISA using the Verikine Mouse Interferon Alpha ELISA Kit (PBL Assay Science, cat. no. KMC4011).
Kallert S.M., Darbre S., Bonilla W.V., Kreutzfeldt M., Page N., Müller P., Kreuzaler M., Lu M., Favre S., Kreppel F., Löhning M., Luther S.A., Zippelius A., Merkler D, & Pinschewer D.D. (2017). Replicating viral vector platform exploits alarmin signals for potent CD8+ T cell-mediated tumour immunotherapy. Nature Communications, 8, 15327.
+ Open protocol
+ Expand
3

Cytokine Detection by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
TNF-α, IL-6, or IL-12p40 were detected by Mouse TNF-α ELISA Ready-SET-Go!, Mouse IL-6 ELISA Ready-SET-Go!, or Mouse IL-12p40 ELISA Ready-SET-Go!, respectively (Thermo Scientific). IFN-α was detected by VeriKine Mouse Interferon Alpha ELISA Kit (PBL Assay Science).
Fukui R., Yamamoto C., Matsumoto F., Onji M., Shibata T., Murakami Y., Kanno A., Hayashi T., Tanimura N., Yoshida N, & Miyake K. (2018). Cleavage of Toll-Like Receptor 9 Ectodomain Is Required for In Vivo Responses to Single Strand DNA. Frontiers in Immunology, 9, 1491.
+ Open protocol
+ Expand
4

Mouse Interferon Alpha ELISA Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
IFNα levels were measured using a VeriKine™ Mouse Interferon Alpha ELISA Kit (PBL Assay Science, Piscataway, NJ) following the manufacturer’s recommendations.
Fernandez-Sendin M., Di Trani C.A., Bella A., Vasquez M., Ardaiz N., Gomar C., Arrizabalaga L., Ciordia S., Corrales F.J., Aranda F, & Berraondo P. (2021). Long-Term Liver Expression of an Apolipoprotein A-I Mimetic Peptide Attenuates Interferon-Alpha-Induced Inflammation and Promotes Antiviral Activity. Frontiers in Immunology, 11, 620283.
+ Open protocol
+ Expand
5

Cytokine Response to Flavivirus Vaccination

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6 mice (n = 10/group) were vaccinated as per immunisation schedule and spleens were collected 3 week post last immunisation. Splenocytes were restimulated with ccJE vaccine (1 μg/106 cells) or flaviviruses (JEV, WNV, DENV-1 or DENV-2) at a MOI of 0.1 for 106 cells for 4 days. Levels of secreted cytokines in culture supernatant was measured using a Bio-Plex Pro Mouse Cytokine 23-Plex Immunoassay (Bio-Rad) and VeriKine Mouse Interferon Alpha ELISA Kit (PBL Assay Science, Piscataway, NJ, USA) according to manufacturers’ instructions.
Komiya T., Honda-Okubo Y., Baldwin J, & Petrovsky N. (2021). An Advax-Adjuvanted Inactivated Cell-Culture Derived Japanese Encephalitis Vaccine Induces Broadly Neutralising Anti-Flavivirus Antibodies, Robust Cellular Immunity and Provides Single Dose Protection. Vaccines, 9(11), 1235.
+ Open protocol
+ Expand
6

Measuring Serum IFNα Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
IFNα levels in serum were measured using the VeriKine™ Mouse Interferon Alpha ELISA kit (PBL, NJ, USA) by following the manufacturer’s recommendations.
Berraondo P., Di Scala M., Korolowicz K., Thampi L.M., Otano I., Suarez L., Fioravanti J., Aranda F., Ardaiz N., Yang J., Kallakury B.V., Tucker R.D., Vasquez M., Menne S., Prieto J, & González-Aseguinolaza G. (2015). Liver-directed gene therapy of chronic hepadnavirus infection using interferon alpha tethered to apolipoprotein A-I. Journal of hepatology, 63(2), 329-336.
+ Open protocol
+ Expand
7

Cytokine Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse and human IFNα was detected using a VeriKine Mouse Interferon Alpha ELISA Kit and VeriKine Human IFN Alpha Multi-Subtype ELISA Kit, respectively (PBL Assay Science). Mouse IL-12p70 was detected using a Quantikine ELISA kit (R&D Systems). Human IL-12p70 was detected using the CBA Enhanced Sensitivity Flex Set (BD Biosciences).
Leylek R., Alcántara-Hernández M., Lanzar Z., Lüdtke A., Perez O.A., Reizis B, & Idoyaga J. (2019). Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell reports, 29(11), 3736-3750.e8.
+ Open protocol
+ Expand
8

Cytokine Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse and human IFNα was detected using a VeriKine Mouse Interferon Alpha ELISA Kit and VeriKine Human IFN Alpha Multi-Subtype ELISA Kit, respectively (PBL Assay Science). Mouse IL-12p70 was detected using a Quantikine ELISA kit (R&D Systems). Human IL-12p70 was detected using the CBA Enhanced Sensitivity Flex Set (BD Biosciences).
Leylek R., Alcántara-Hernández M., Lanzar Z., Lüdtke A., Perez O.A., Reizis B, & Idoyaga J. (2019). Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell reports, 29(11), 3736-3750.e8.
+ Open protocol
+ Expand
9

Cytokine Profiling in Murine Serum

Check if the same lab product or an alternative is used in the 5 most similar protocols
The concentration of IL-15 in serum was determined using the Mouse IL-15/IL-15R Complex ELISA (enzyme-linked immunosorbent assay) Ready-SET-Go! set (eBioscience), and the serum IFN-α concentration was determined using the VeriKine Mouse Interferon Alpha ELISA kit (PBL Assay Science). HBsAg levels were also determined by ELISA (Bioelisa HBsAg 3.0; Biokit).
Di Scala M., Otano I., Gil-Fariña I., Vanrell L., Hommel M., Olagüe C., Vales A., Galarraga M., Guembe L., Ortiz de Solorzano C., Ghosh I., Maini M.K., Prieto J, & González-Aseguinolaza G. (2016). Complementary Effects of Interleukin-15 and Alpha Interferon Induce Immunity in Hepatitis B Virus Transgenic Mice. Journal of Virology, 90(19), 8563-8574.
+ Open protocol
+ Expand
10

Quantification of Lung Cytokines and Interferons

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lung tissue portions (right inferior lobes) were thawed and homogenized in 500 μl of PBS containing 0.5% BSA and a protease inhibitor cocktail (CompleteTM, Mini, EDTA-free, Sigma-Aldrich) by using a TissueLyser II at 30-Hz oscillation frequency for 3 min. Homogenates were centrifuged (10,000 × g for 10 min) to remove debris, and cleared homogenate supernatants were used for cytokine quantification with the Bio-Plex ProTM Mouse Cytokine 23-plex Assay (Bio-Rad; assayed cytokines include CCL11, CSF3, CSF2, IFNG, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL9, IL10, IL12A, IL12B, IL13, IL17A, CXCL1, CCL2, CCL3, CCL4, CCL5, and TNF) and the Bio-PlexTM 200 system according to the manufacturer’s instructions. Cleared homogenate supernatants also were used for type I interferon quantification with the VeriKine Mouse Interferon Alpha ELISA kit (PBL Assay Science) and the VeriKine Mouse Interferon Beta ELISA Kit (PBL Assay Science) according to the manufacturer’s instructions. ELISAs were evaluated with a Tecan Infinite M1000 plate reader.
Eisfeld A.J., Gasper D.J., Suresh M, & Kawaoka Y. (2019). C57BL/6J and C57BL/6NJ Mice Are Differentially Susceptible to Inflammation-Associated Disease Caused by Influenza A Virus. Frontiers in Microbiology, 9, 3307.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!