For quantification of confocal images, the ‘Histo’ function of Zen (2012) imaging software (Carl Zeiss) was used. Region of interest was outlined for each disc and mean fluorescence signal intensity was determined. Crosses were repeated at least three times. Analysis and graph generation was done using GraphPad Prism 9. The statistical method used was one-way ANOVA with Holm-Sidak test for multiple comparisons, unless otherwise indicated. Plotted is mean intensity ±SEM. Levels of significance are depicted by asterisks in the figures: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
N numbers are as follows:
Figure 1O : 4, 8, 4, 7 (from left to right)
Figure 1P : 5, 6, 6, 5 (from left to right)
Figure 2N : 4, 4, 6, 6 (from left to right)
Figure 2O : 7, 12, 7, 6 (from left to right)
Figure 3F : 7, 9, 12 (from left to right)
Figure 3M : 4, 4, 6, 7 (from left to right)
Figure 3N : 5, 11, 10, 4 (from left to right)
Figure 6I : 7, 15, 4, 17 (from left to right)
Figure S3F : 3, 6, 5, 6, 7 (from left to right)
Figure S4G : 8, 9, 11 (from left to right)
Figure S7B : 50, 50 (from left to right)
N numbers are as follows: