Enhanced chemiluminescence ecl western blotting kit

The RPMI-1640 and fetal bovine serum (FBS) were purchased from HyClone Laboratories (South Logan, UT, USA). The 3-BrPA and 5-fluorouracil (5-FU) were purchased from Sigma (cat. nos. BCBD0244V and 097K1352; St. Louis, MO, USA). The UltraSensitive™ streptavidin-peroxidase (SP) immunohistochemical kit (Histostain-Plus kits; cat. no. I003-2) and HK kit were purchased from Nanjing Jiancheng Technology Co., Ltd. (cat. no. A077-1; Nanjing, China). The rabbit anti-caspase-3 (P17KD) polyclonal antibodies (1:200) were purchased from Abcam (cat. no. ab4051; Cambridge, UK). The mouse anti-human Bcl-2 monoclonal antibodies (1:200) and the secondary goat anti-mouse immunoglobulin G horseradish peroxidase (HRP)-conjugated antibodies (1:10,000) were purchased from Santa Cruz Biotechnology Inc. (cat. nos. SC-7382 and SC-2005; Santa Cruz, CA, USA). The mouse anti-human Bax monoclonal antibodies (1:5,000) were obtained from Invitrogen (cat. no. AHO0112; Carlsbad, CA, USA). The reference mouse anti-human GAPDH monoclonal antibodies (1:10,000) were purchased from Shanghai Kangcheng Biological Engineering Co., Ltd. (cat. no. KC-5G5; Shanghai, China), and the enhanced chemiluminescence (ECL) western blotting kit was obtained from Thermo Scientific (cat. no. 32109; Waltham, MA, USA).