Sperm T-AOC was measured using the Total Antioxidant Capacity Assay Kit with a Rapid ABTS method (Beyotime Institute of Biotechnology, Shanghai, China). Briefly, the incubated semen was rinsed three times with PBS and resuspended before being lysed ultrasonically (20 kHz, 300 W, operating at 100%, on 3 s, off 5 s, 7 cycles) on ice and centrifuged at 12,000× g for 10 min at 4 °C. The supernatants were collected, and sperm T-AOC was measured according to the manufacturer’s procedure by a spectrophotometer (Spectrophotometer 1510, Thermo Fisher Scientific, Waltham, MA, USA) at 414 nm. Quantification of the extracted sperm proteins was measured by spectrophotometer at 562 nm using BCA Protein Assay Kit (KeyGEN BioTECH, Nanjing, China). T-AOC of each sperm sample was converted into mmol per g of total protein in spermatozoa and expressed as mmol/g. Each group included at least three replicates.
Total antioxidant capacity assay kit with a rapid abts method
Manufactured by Beyotime
Sourced in China
The Total Antioxidant Capacity Assay Kit with a Rapid ABTS method is designed to quantitatively measure the total antioxidant capacity of various samples, including food, plant extracts, and biological fluids. The assay is based on the ABTS+ (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging method, which provides a rapid and sensitive way to determine the antioxidant capacity of samples.
Automatically generated - may contain errors
Lab products found in correlation
Multimode reader, by Thermo Fisher Scientific (2 mentions)
Spectrophotometer 1510, by Thermo Fisher Scientific (1 mentions)
Bca protein assay kit, by Keygen Biotech (1 mentions)
Microplate reader, by Agilent Technologies (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Nanjing Jiancheng (1 mentions)
5 protocols using total antioxidant capacity assay kit with a rapid abts method
1
Sperm Antioxidant Capacity Measurement
2
Rapid ABTS Antioxidant Capacity Assay
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Total antioxidant capacity assay kit with a rapid ABTS method (Beyotime Institute of Biotechnology, China) was used to evaluate the total antioxidant capacity based on the manufacturer’s instructions. Samples were incubated at 25 °C for 6 min and then were recorded at 414 nm using a multimode reader (Thermo Fisher Scientific, USA).
3
Rapid ABTS Total Antioxidant Capacity Assay
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The Total Antioxidant Capacity Assay Kit with a Rapid ABTS method (Beyotime, Shanghai, China) was used to detect the T-AOC by following the manufacturer’s protocol. The gastrocnemius tissue or cells were lysed with RAPI lysate, the supernatant was collected after centrifugation for determination, the absorbance at 414 nm was measured using a microplate reader (BioTek, Vermont, USA), and finally, the T-AOC in the sample was calculated.
4
Antioxidant Evaluation of PDA-PEG Nanoparticles
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The antioxidant activities of PDA-PEG NPs in water and organic solvent were evaluated by ABTS and DPPH assay, respectively. A total antioxidant capacity assay kit with a rapid ABTS method was purchased from Beyotime Biotechnology (China). The green ABTS•+ could be catalyzed to form ABTS in the presence of antioxidants. The degree of discolouration was quantified as a drop in the absorbance of 734 nm [61 (link)]. The antioxidant activities of PDA-PEG NPs were calculated referring to Trolox, which was used as a standard. DPPH (Nanjing Jiancheng Bioengineering Institute, China) was dissolved in 80% methanol. PDA and PDA-PEG NPs at various concentrations (0, 25, 50, and 100 µg/ml) were mixed with 100 µL DPPH solution and incubated for 30 min in the dark. The absorbance of reaction mixtures was detected at a wavelength of 517 nm. The radical scavenging activity was calculated referring to a previous study [62 (link)].
5
Rapid ABTS Total Antioxidant Assay
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The assays for total antioxidant capacity were carried out as described previously [18 (link)]. A total antioxidant capacity assay kit with a rapid ABTS method (Beyotime Institute of Biotechnology, Shanghai, China) was used to evaluate the total antioxidant capacity based on the manufacturer’s instructions. Samples were incubated at 25 °C for 6 min and then were recorded at 414 nm using a multimode reader (Thermo Fisher Scientific, Waltham, MA, USA).
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