TNFα, MPO and hsCRP were measured by ELISA kits (TNF-apha Human, High sensitivity; Myeloperoxidase Human Instant and CRP Human, produced by Invitrogen by Thermo Fisher Scientific, MA, USA). hsTnT, BNP and NT-pro BNP were assayed by ECLIA methodology using commercial kits (Elecsys Troponin T hs, Elecsys BNP, Elecsys proBNP II, respectively) from Roche Diagnostics S.p.A., Milan (Italy) on the COBAS analyser e411. Mid-regional proADM and NT-PRO3 by ELISA kits (Human MR-ProADM and Human Procollagen III N-Terminal Propeptide) produced by MYBIOSOURCE, CA (USA).
Elecsys probnp 2
Manufactured by Roche
Sourced in Germany, Switzerland, United States
The Elecsys proBNP II is a laboratory test used to measure the levels of the B-type Natriuretic Peptide (BNP) precursor in a patient's blood sample. It is a quantitative electrochemiluminescence immunoassay intended for use on the Roche Elecsys and cobas e immunoassay analyzers.
Automatically generated - may contain errors
Lab products found in correlation
Elecsys troponin t gen 5 stat, by Roche (2 mentions)
Human mr proadm, by MyBioSource (1 mentions)
Human procollagen 3 n terminal propeptide, by MyBioSource (1 mentions)
Elecsys troponin t hs, by Roche (1 mentions)
Quantikine elisa human gdf 15, by R&D Systems (1 mentions)
Cobas e801 analyzer, by Roche (1 mentions)
Elisa assays, by R&D Systems (1 mentions)
Cobas e immunoassay analyzer, by Roche (1 mentions)
Troponin t stat immunoassay, by Roche (1 mentions)
Cardiophase hscrp, by Siemens (1 mentions)
Elecsys 2010 system, by Roche (1 mentions)
Elecsys troponin t, by Roche (1 mentions)
Ransel test kit, by Randox (1 mentions)
21 protocols using elecsys probnp 2
1
Biomarker Quantification via ELISA and ECLIA
2
Comprehensive Biomarker Assessment in Blood
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Venous blood was collected in K2EDTA tubes for assessment of hematology and glycated hemoglobin (HbA1c), Serum Separator Tubes (SSTs) and Gel and Clot Activator Tubes for assessment of clinical chemistry, and sodium fluoride tubes for assessment of glucose at the Clinical Chemistry Laboratory of Leiden University Medical Center (Leiden, The Netherlands). Additional venous blood was collected in K2EDTA tubes for assessment of plasma biomarkers growth/differentiation factor 15 (GDF‐15; ELISA, Quantikine ELISA Human GDF‐15, R&D Systems), pentraxin 3 (PTX 3; ELISA, Quantikine ELISA human Pentraxin 3/TGS‐4, R&D Systems), interleukin‐6 (IL‐6; ECLIA, Proinflammatory Panel 1 (human) Kit, Meso Scale Discovery), N‐terminal prohormone of brain natriuretic peptide (NT‐proBNP; ECLIA, Elecsys proBNP II, Roche Diagnostics), cardiac troponin I (cTNI; CLEIA, Lumipulse® G hs Troponin I, Fujirebio). and high‐sensitivity C‐reactive protein (hsCRP; Immunoturbidimetric Test Kit, CRP4, Roche Diagnostics) at MLM Medical Labs GmbH (Mönchengladbach, Germany). All blood collection was performed in a fasted state after an overnight fast.
3
Maternal NT-proBNP Levels in Pregnancy
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Maternal blood samples were collected at two distinct stages: early pregnancy (7–13 weeks) and late pregnancy (35–37 weeks) using standard venipuncture methods. The patients provided nonfasting blood samples. At the Keihin Medical Laboratory (Kawasaki, Kanagawa, Japan), NT-proBNP levels were measured using the Roche Elecsys proBNP II electrochemiluminescence sandwich immunoassay with a Cobas e 801 analyzer (Roche Diagnostics, Rotkreuz, Switzerland), according to the manufacturer’s instructions. The NT-proBNP levels ranged from 5 to 35,000 pg/mL. If the NT-proBNP levels exceeded 125 pg/mL, which is the threshold for nonpregnant adults, the patients underwent additional cardiovascular evaluations, including echocardiography.
4
Serum NT-proBNP Measurement Protocol
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NT-proBNP levels were measured in serum using a commercially available kit (Elecsys proBNPII., Roche Ltd., Mannheim, Germany) according to the manufacturer’s instructions.
5
Plasma Biomarkers for Heart Disease
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Plasma total BNP and pro-BNP were measured using CLEIAs as previously described.5 12 (link) NT-pro-BNP concentrations were measured using the Elecsys pro-BNP II assay (Roche Diagnostics, Basel, Switzerland). Details of the measurement procedures are described in the online supplementary methods .
6
Measuring Plasma Catestatin and NT-proBNP
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Plasma levels of catestatin were measured by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instruction (Cat. # EK-053-29, ELISA kit, Phoenix Pharmaceutical Inc., Burlingame, CA). The minimal detection limits for catestatin was 0.06 ng/ml. Serum NT-proBNP levels were measured by a two-site sandwich electrochemiluminescence immunoassay (Elecsys proBNP II, Roche Diagnostic, Mannheim, Germany). These assays were performed by an investigator blinded to the sources of the samples.
7
Serum NT-proBNP Measurement in Dialysis Patients
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Baseline demographic and medical histories were recorded. Blood samples were taken before dialysis on the day of a mid-week dialysis session. The patient’s vital signs were measured in a stable state and at the start of dialysis. Only serum NT-proBNP was taken at the end of HD according to the dry weight of patients before discontinuation of the extra-corporeal circulation. One month later, NT-proBNP was re-assessed in the same manner.
NT-proBNP was measured using Elecsys proBNP II (Roche Diagnostics, Mannheim, Germany). The cutoff used was 125 pg/mL based on the manufacturer’s instructions. The detection range was 5 to 100,000 pg/mL and the coefficient of variation (20%) was <50 pg/mL. Blood samples were stored at 2 to 8 °C.
NT-proBNP was measured using Elecsys proBNP II (Roche Diagnostics, Mannheim, Germany). The cutoff used was 125 pg/mL based on the manufacturer’s instructions. The detection range was 5 to 100,000 pg/mL and the coefficient of variation (20%) was <50 pg/mL. Blood samples were stored at 2 to 8 °C.
8
NT-proBNP Serum Level Measurement
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The study outcome was the serum level of NT‐proBNP measured from the serum samples stored at −80°C at baseline. NT‐proBNP was measured by immunoassay with Elecsys proBNP II (Roche Diagnostics). We included cases in which no stored blood remained after several blood tests as a sample with missing NT‐proBNP data.
9
Serum NT-proBNP Quantification
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NT-proBNP levels were measured in serum using a commercially available kit (Elecsys proBNP II., Roche Ltd., Mannheim, Germany).
10
Measuring Cytokines and Cardiac Biomarkers
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Tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) levels were measured by ELISA assays (R&D Systems) as described previously [23 , 26 ]. NT-proBNP was measured by two-site sandwich electrochemiluminescence immunoassay (Elecsys proBNP II; Roche, Mannheim, Germany) [25 (link)].
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