Rabbit polyclonal anti gfp

Manufactured by Santa Cruz Biotechnology

Rabbit polyclonal anti-GFP is a laboratory reagent used to detect and quantify the presence of green fluorescent protein (GFP) in various biological samples. It is a polyclonal antibody raised in rabbits that specifically binds to GFP, allowing for its identification and localization within cells or tissues.

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Lab products found in correlation

Mouse monoclonal anti neun, by Merck Group (1 mentions) Alexa fluor 488 goat anti rabbit igg, by Abcam (1 mentions) Alexa fluor 488 goat anti rat igg, by Abcam (1 mentions) Alexa fluor 594 donkey anti rabbit igg, by Abcam (1 mentions) Ix81 zdc2, by Olympus (1 mentions) Alexa fluor 594 goat anti mouse igg, by Abcam (1 mentions) Axio imager m2 imaging system, by Zeiss (1 mentions) Alexa 568, by Thermo Fisher Scientific (1 mentions) Lsm 700 confocal imaging system, by Zeiss (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Anti dsred, by Takara Bio (1 mentions) Anti flag, by Santa Cruz Biotechnology (1 mentions) Anti gfp, by Santa Cruz Biotechnology (1 mentions) Mouse monoclonal anti β actin, by Merck Group (1 mentions) Mouse monoclonal anti flag, by Merck Group (1 mentions)

3 protocols using rabbit polyclonal anti gfp

Immunohistochemical Analysis of Cell Markers

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Immunohistochemical staining was performed as previously reported (Zhang et al., 2015). On day 7 or 21 following PCI, the brains were removed, and 5-µm frozen coronal sections were prepared. After antigen retrieval, sections were immunolabeled with rat monoclonal anti-BrdU (1:500; Accurate Chemical & Scientific), rabbit polyclonal anti-GFP (1:200; Santa Cruz Biotechnology), mouse monoclonal anti-NeuN (1:500; Chemicon), rabbit monoclonal anti-GFAP (1:200; Chemicon), mouse monoclonal anti-RIP (1:50; kind gift from Dr Xu XM, University of Louisville School of Medicine) and mouse monoclonal anti-NgR1 (1:200; Biogen Idec, Inc.) overnight at 4°C. After washing with PBS, the sections were incubated with goat anti-rabbit IgG Alexa Fluor 488, goat anti-rat IgG Alexa Fluor 488, goat anti-mouse IgG Alexa Fluor 594 and donkey anti-rabbit IgG Alexa Fluor 594 (1:1000; Abcam) at room temperature for 1 hour. Cells double-positive for BrdU/NeuN, GFP/GFAP and GFP/RIP were counted. Positive cells were also visualized using a motorized inverted microscope (IX81-ZDC2; Olympus, Hamburg, Germany).

He H.W., Zhang Y.L., Yu B.Q., Ye G., You W., So K.F, & Li X. (2019). Soluble Nogo receptor 1 fusion protein protects neural progenitor cells in rats with ischemic stroke. Neural Regeneration Research, 14(10), 1755-1764.

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Worm Immunostaining of Tubulin Modifications

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Worm immunostaining was performed as described [50] (link). Briefly, mixed-stage animals were flash-frozen in liquid nitrogen and fixed in 2% paraformaldehyde on ice for at least 4 hours, permeabilized by Tris-Triton, and subjected to series of reduction and oxidation by sequential β-mercaptoethanol, dithiothreitol (DTT) and hydrogen peroxide treatment in 1% borate base buffer, and stained with primary antibodies in PBST-A. The following primary antibodies were used in this study: 6-11B-1 (mouse monoclonal anti-K40 acetylated α-tubulin, 1∶500, Santa Cruz Biotech), GT335 (mouse monoclonal anti-polyglutamylated tubulin, 1∶100, Enzo Life Sciences), rabbit polyclonal anti-detyrosinated tubulin (1∶200, Millipore), YL1/2 (rat monoclonal anti-tyrosinated tubulin, 1∶200, Santa Cruz Biotech), and rabbit polyclonal anti-GFP (1∶250, Santa Cruz Biotech). Secondary antibodies are goat anti-rabbit, goat anti-rat or goat anti-mouse IgG conjugated with Alexa488 or Alexa568 used at 1∶100 (Molecular Probes). Animals were counterstained with DAPI at 1∶1000 diluation in 2% n-propylgallate (NPG) and observed with the Zeiss AxioImager M2 imaging system. For fluorescence confocal microscopy, L4 to young adult hermaphrodite animals were anesthetized with 1% sodium azide, mounted on agar pad, and observed under Zeiss LSM700 confocal imaging system.

Hsu J.M., Chen C.H., Chen Y.C., McDonald K.L., Gurling M., Lee A., Garriga G, & Pan C.L. (2014). Genetic Analysis of a Novel Tubulin Mutation That Redirects Synaptic Vesicle Targeting and Causes Neurite Degeneration in C. elegans. PLoS Genetics, 10(11), e1004715.

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Antibody Validation and Affinity Purification

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The antibodies employed were rabbit polyclonal anti-BCR (Santa Cruz), rabbit polyclonal anti-TANC2 (Bethyl), polyclonal rabbit-anti USP9X (Abcam), mouse monoclonal anti-β-actin (Sigma), mouse monoclonal anti-Flag (Sigma), rat monoclonal anti-HA-peroxidase (Roche), rabbit polyclonal anti-dsRed from Clontech (detects mCherry) mouse monoclonal anti-Myc (Santa Cruz), rabbit polyclonal anti-GFP (Santa Cruz). Anti-rabbit and anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibodies (Thermo Scientific). The agarose antibody-conjugated beads were protein A/G beads (Pierce), anti-Flag (Santa Cruz), and anti-GFP

Paemka L., Mahajan V.B., Ehaideb S.N., Skeie J.M., Tan M.C., Wu S., Cox A.J., Sowers L.P., Gecz J., Jolly L., Ferguson P.J., Darbro B., Schneider A., Scheffer I.E., Carvill G.L., Mefford H.C., El-Shanti H., Wood S.A., Manak J.R, & Bassuk A.G. (2015). Seizures Are Regulated by Ubiquitin-specific Peptidase 9 X-linked (USP9X), a De-Ubiquitinase. PLoS Genetics, 11(3), e1005022.

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