Multiskan mk3 thermo labsystems microplate reader

The concentrations of glucose (Glu), non-esterified fatty acid (NEFA), triglycerides (TG), total cholesterol (TC), and urea in plasma were detected by commercial kits according to the manufacturer’s instructions (Nanjing Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China). Optical density (OD) values were determined at 520 nm, 440 nm, 546 nm, and 510 nm by a MuLtisKan MK3-Thermo Labsystems microplate reader (Thermo Labsystems, CA, USA). Plasma zonulin and endotoxin were measured using sow enzyme-linked immunosorbent assay (ELISA) Kits (R&D Systems Inc., Minneapolis, MN, USA). Optical density (OD) values were determined at 450 nm by a MuLtisKan MK3-Thermo Labsystems microplate reader (Thermo Labsystems, CA, USA). Minimal detection limits for Glu, NEFA, TG, TC, urea, endotoxin, and zonulin were 0.5 mmol/L, 10 μmol/L, 0.02 mmol/L, 0.1 mmol/L, 0.1 mmol/L, 3.1 ng/L, and 3 ng/L.

The concentrations of triglycerides (TGs), nonesterified fatty acid (NEFA), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in plasma were detected by commercial kits according to the manufacturer's instructions (Nanjing Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China; catalog no. A110-1-1, A042-2-1, A111-2-1, A113-2-1, A112-2-1, respectively). Optical density (OD) values were determined at 520, 440, 546, and 510 nm by a MuLtisKan MK3-Thermo Labsystems microplate reader (Thermo Labsystems, CA, USA). Serum estradiol, prolactin, testosterone, and progesterone were measured using enzyme-linked immunosorbent assay kits (R&D Systems Inc., Minneapolis, MN, USA; catalog no. KGE014, DPRL00, KGE010, DYC5415-2, respectively). OD values were determined at 450 nm by a MuLtisKan MK3-Thermo Labsystems microplate reader (Thermo Labsystems). Minimal detection limits for TG, NEFA, TC, LDL-C, HDL-C, estradiol, prolactin, testosterone, and progesterone were 0.02 mmol/L, 10 μmol/L, 0.1 mmol/L, 0.01 mmol/L, 0.01 mmol/L, 1 pg/mL, 10 mIU/L, 1 pg/L, and 10 pg/mL.