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14 gauge angiocatheter

Manufactured by Terumo
Sourced in Japan

The 14-gauge angiocatheter is a medical device used for intravenous (IV) access. It is designed to facilitate the administration of fluids, medications, or blood products directly into a patient's vein.

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3 protocols using 14 gauge angiocatheter

1

Myocardial Infarction Model in Rats

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A myocardial infarction model was prepared using eight-week-old male SD rats (weight: 240–280 g). After anesthetization with an intra-peritoneal injection of sodium pentobarbital (Somnopentyl, 30 mg/kg; Kyoritsu Seiyaku, Tokyo, Japan), the rat was intubated with a 14-gauge angiocatheter (Terumo, Tokyo, Japan). Artificial ventilation (volume of 4.5 mL and respiration rate of 70 breaths/min) was next connected to the gauge. A lateral incision was made to approach the heart, followed by ligation of the left coronary artery with a 7-0 monofilament suture (Prolene; Ethicon Japan, Tokyo, Japan). Immediately after ligation, 2.0 × 105 c-kit+ or c-kit− cells were injected into the peri-infarct area, using a 26-gauge needle. To maximize the effect of the cells, they were injected in 70 µL of a thermosensitive and biodegradable hydrogel. This gel has been described previously [19 ,20 (link)]. For a control group, we injected the same volume of the hydrogel with no cells.
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2

Myocardial Infarction Model in Rats

Check if the same lab product or an alternative is used in the 5 most similar protocols
A myocardial infarction model was prepared using eight-week-old male SD rats (weight: 240–280 g). After anesthetization with an intra-peritoneal injection of sodium pentobarbital (Somnopentyl, 30 mg/kg; Kyoritsu Seiyaku, Tokyo, Japan), the rat was intubated with a 14-gauge angiocatheter (Terumo, Tokyo, Japan). Artificial ventilation (volume of 4.5 mL and respiration rate of 70 breaths/min) was next connected to the gauge. A lateral incision was made to approach the heart, followed by ligation of the left coronary artery with a 7-0 monofilament suture (Prolene; Ethicon Japan, Tokyo, Japan). Immediately after ligation, 2.0 × 105 c-kit+ or c-kit− cells were injected into the peri-infarct area, using a 26-gauge needle. To maximize the effect of the cells, they were injected in 70 µL of a thermosensitive and biodegradable hydrogel. This gel has been described previously [19 ,20 (link)]. For a control group, we injected the same volume of the hydrogel with no cells.
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3

Cardiac Infarct Cell Therapy

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Male Sprague-Dawley rats at 8 weeks of age (weight: 240–280 g) were anesthetized by intraperitoneal injection of sodium pentobarbital (Somnopentyl; Kyoritsu Seiyaku, Tokyo, Japan; 30 mg/kg). After anesthesia, each rat was intubated with a 14-gauge angiocatheter (Terumo, Tokyo, Japan). Artificial ventilation was then started with a tidal volume of 4.5 mL and a respiration rate of 70 breaths/min. A lateral incision was made to approach the heart, followed by ligation of the coronary artery with a 7-0 monofilament suture (Prolene; Johnson and Johnson Japan, Tokyo, Japan). Immediately after ligation, 70 µL of cells only (CO) or cells suspended in hydrogel (HG) were injected into the peri-infarct area with a 26-gauge needle. As a control (CTL), an equivalent volume of PBS was injected.
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