Peroxidase conjugated secondary antibodies for western blot analysis

All reagents and media were purchased from Sigma Chemical Co. (St. Louis-MO, USA). Primary antibodies against Calnexin (H-70), GAPDH (FL-335), AKT (C-20), CaMKII (M-176), pCaMKIIα (Thr286), ERK1 (K-23), ERK2 (C-14), pERK1/2 (E-4), OPN (K-20), and SOD2 (FL-222) were purchased from Santa Cruz Biotechnology (Heidelberg, Germany). Primary antibody against Phospho-AKT (Ser473-D9E-XP™) was purchased from Cell Signaling Technology (Danvers-MA, USA). Peroxidase-conjugated secondary antibodies for Western blot analysis and FITC- or Rhodamine-conjugated antibodies for immunofluorescence study were purchased from Santa Cruz Biotechnology (Heidelberg, Germany). Fluorescently-labeled Phalloidin (AlexaFluor®488-Invitrogen) was purchased from Life Technologies (Carlsbad, CA, USA). CytoPainter Mitochondrial Staining Kit–Green (AB 112143) was purchased from Prodotti Gianni (Milano, Italy), and Cell ROX® Oxidative Stress Reagents Kit (C10443) was purchased from Thermo Fisher Scientific, Life Technologies Italia (Monza, Italy).

Fura-2 acetoxymethyl ester (Fura-2/AM) was from Calbiochem (La Jolla, CA, USA). Bis-(1,3-dibutylbarbituric acid) trimethine oxonol, DiBAC₄-(3), was obtained from molecular probes (Eugene, OR, USA). All reagents, including materials for video imaging experiments, were purchased from Sigma Chemical Co. (St. Louis-MO, USA).
Primary antibodies against: Calnexin (H-70), GAPDH (FL-335), CaMKII (M-176), pCaMKII (Thr286), p70 S6 (C-18), pp70 S6 (C-18), ERK1 (K-23), ERK2 (C-14), pERK1/2 (E-4), OPN (K-20), peroxidase-conjugated secondary antibodies for western blot analysis and FITC or Rhodamine-conjugated antibodies for immunofluorescence study were purchased from Santa Cruz Biotechnology (Heidelberg, Germany). AlexaFluor^®488-Phalloidin was obtained from Life Technologies (Carlsbad-CA, USA).

C2C12 mouse cells were purchased from the European Collection of Animal Cell Cultures (ECACC), reagents from Sigma Chemical Co. (St. Louis-MO, USA).
Primary antibodies against: Calnexin (H-70), GAPDH (FL-335), AKT (C-20), CaMKII (M-176), pCaMKIIalpha (Thr286), MyHC (H-300), Myf5 (C-20), MyoD (C-20), Myogenin (D-10), p53 (FL-393), p70S6 (C-18), pp70S6 (C-18), ERK1 (K-23), ERK2 (C-14), pERK1/2 (E-4), p21 (C-19), OPN (K-20), peroxidase-conjugated secondary antibodies for Western blot analysis and Rhodamine-conjugated antibodies for Immunofluorescence studies were purchased from Santa Cruz Biotechnology (Santa Cruz-CA, USA). Primary antibody Phospho-AKT (Ser473-D9E-XP^TM) was purchased from Cell Signaling Technology (Danvers-MA, USA).
Fluorescently-labeled phalloidin (AlexaFluor^®488-Invitrogen) was purchased from Life Technologies (Carlsbad-CA, USA).
CytoPainter Mitochondrial Staining Kit-Green (AB 112143) was purchased from Prodotti Gianni (Milano-Italy), Cell ROX^® Oxidative Stress Reagents Kit (C10443) from Thermo Fisher Scientific, Life Technologies Italia (Monza-Italy).