Selleckchem supplied R428 and cisplatin (Houston, TX, USA), and R&D system supplied recombinant human recombinant TRAIL and z-VAD-fmk (Minneapolis, MN, USA). Sigma Chemical Co. provided MG132, cycloheximide, carboplatin, oxaliplatin, doxorubicin, and 5-FU (St. Louis, MO, USA). The primary antibodies were as follows: Anti-phospho-Axl (Y702) (Cell Signaling Technology, Beverly, MA, USA), anti-pro-caspase-3 (Cell Signaling Technology), anti-cleaved caspase-3 (Cell Signaling Technology), anti-PARP (Cell Signaling Technology), anti-Bcl-xL (Cell Signaling Technology), anti-DR5 (Cell Signaling Technology), anti-actin (Sigma Chemical Co.), anti-Axl (Santa Cruz Biotechnology, St. Louis, MO, USA), anti-Mcl-1 (Santa Cruz Biotechnology), anti-Bcl-2 (Santa Cruz Biotechnology), anti-cIAP2 (Santa Cruz Biotechnology), anti-Bim (BD Biosciences, San Jose, CA, USA), anti-XIAP (BD Biosciences), anti-survivin (R&D system, Minneapolis, MN, USA), anti-DR4 (Abcam, Cambridge, MA, USA), and anti-c-FLIP (Enzo Life Sciences, San Diego, CA, USA). The siRNAs were as follows: GFP (control) siRNA (Bioneer, Daejeon, Korea), Axl siRNA (Santa Cruz Biotechnology), and DR5 siRNA (Invitrogen).
Axl sirna
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Axl siRNA is a laboratory reagent designed for the specific knockdown of the Axl gene expression in cell-based experiments. It is a small interfering RNA (siRNA) molecule that targets the Axl mRNA, leading to its degradation and reduced protein production.
Automatically generated - may contain errors
Lab products found in correlation
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Anti actin, by Merck Group (1 mentions)
Mg132, by Merck Group (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Oxaliplatin, by Merck Group (1 mentions)
Carboplatin, by Merck Group (1 mentions)
Z vad fmk, by R&D Systems (1 mentions)
Gfp control sirna, by Bioneer (1 mentions)
Anti bim, by BD (1 mentions)
Anti bcl 2, by Santa Cruz Biotechnology (1 mentions)
Cisplatin, by R&D Systems (1 mentions)
Anti mcl 1, by Santa Cruz Biotechnology (1 mentions)
Anti pro caspase 3, by Cell Signaling Technology (1 mentions)
Anti survivin, by R&D Systems (1 mentions)
Anti phospho axl y702, by Cell Signaling Technology (1 mentions)
Dr5 sirna, by Thermo Fisher Scientific (1 mentions)
Anti dr4, by Abcam (1 mentions)
Anti ciap2, by Santa Cruz Biotechnology (1 mentions)
Anti c flip, by Enzo Life Sciences (1 mentions)
2 protocols using axl sirna
1
Molecular Mechanisms of TRAIL-Induced Apoptosis in Cancer Cells
2
Axl and ZEB1 Silencing in GC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GC cells were transfected with Axl siRNA, ZEB1 siRNA (Santa Cruz Biotechnology, Santa Cruz, CA, USA) or pCMV3-Axl plasmid (GenePharm, Shanghai, China) by using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacture’s protocol. Scrambled sequence serves as a control siRNA (siNC) and pCMV3 Vector was used as a control plasmid. After a 6-h transfection, medium was refreshed. Cells were further cultured for 24 h and then performed as indicated.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!