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Bio plex multiplex bead array system

Manufactured by Bio-Rad
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The Bio-Plex multiplex bead array system is a platform for quantitative and qualitative analysis of multiple analytes in a single sample. It utilizes fluorescently-labeled, color-coded magnetic beads to enable simultaneous detection and measurement of up to 500 different analytes in a single well.

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Lab products found in correlation

Nupage system, by Thermo Fisher Scientific (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Novex zymogram system, by Thermo Fisher Scientific (1 mentions) Multiplex array kit, by Bio-Rad (1 mentions) Topcount nxt microplate scintillation and luminescence counter, by PerkinElmer (1 mentions)

Close spelling variants of this product

Bio-Plex (286 citations) Bio-Plex system (162 citations) Bio-Beads (148 citations) Bio-Plex Multiplex System (44 citations) Multiplex bead array (6 citations) Bio-Plex bead array (2 citations)

5 protocols using bio plex multiplex bead array system

1

Protein Expression and Cytokine Profiling

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Immunoblots for Jnk (rabbit polyclonal antibody, Abcam), phospho-Jnk (clone 98F2, Cell Signaling Technology), Stat3 (rabbit polyclonal antibody, Cell Signaling Technology), phospho-Stat3 (clone D3A7, Cell Signaling Technology), and Lox (rabbit polyclonal antibody, Abcam) were performed using the NuPAGE system (ThermoFisher Scientific). Gelatin zymography was performed using the Novex zymogram system (ThermoFisher Scientific) according to the manufacturer's instruction. Serum cytokine concentrations were quantified using a Bio-Plex multiplex bead array system with the mouse cytokine Th17 panel A (#M6000007NY, Bio-Rad).
Nishihara M., Aoki H., Ohno S., Furusho A., Hirakata S., Nishida N., Ito S., Hayashi M., Imaizumi T, & Fukumoto Y. (2017). The role of IL-6 in pathogenesis of abdominal aortic aneurysm in mice. PLoS ONE, 12(10), e0185923.
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2

Multiplex Cytokine Profiling in Serum and Skin

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The levels of inflammatory cytokines such as interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α, in serum and skin lysate were measured. Moreover, the levels of Th1 cytokines including IL-2, IL-12 (p70), and interferon gamma (IFN)-γ, as well as Th2 cytokines including IL-4 and IL-10, were measured by using a multiplex array kit (Bio-Rad, San Diego, CA, USA) and run on Luminex technology (Bio-Plex Multiplex Bead Array System™, Bio-Rad, Hercules, CA, U.S.A.) according to manufacturer’s instruction. Raw fluorescence data were analyzed by using a five-parameter logistic method.
Bajgai J., Xingyu J., Fadriquela A., Begum R., Kim D.H., Kim C.S., Kim S.K, & Lee K.J. (2021). Effects of mineral complex material treatment on 2,4- dinitrochlorobenzene-induced atopic dermatitis like-skin lesions in mice model. BMC Complementary Medicine and Therapies, 21, 82.
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3

B7-H3 CAR CD8+ T Cell Cytotoxicity

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B7-H3CAR CD8+ T cell cytotoxicity was determined by chromium release assay. Target cells were labeled with 51Cr (Perkin Elmer), washed, and incubated with T cells at various effector to target (E:T) ratios. Supernatants were harvested 4 hours later for γ-counting using a TopCount NXT Microplate scintillation and Luminescence counter (Perkin Elmer) and specific lysis was calculated using the standard formula(63 (link)). Cytokine release assay: To investigate cytokine secretion, B7-H3CAR CD8+ T cells and target cells were plated at a 2:1 ratio and supernatant was analyzed for IL-2, IFN-γ and TNF-α production after 24-hour incubation using the Bio-Plex multiplex bead array system (Bio-Rad). In vitro cytotoxicity and cytokine release assays were performed in triplicate and repeated for validity.
Vitanza N.A., Wilson A.L., Huang W., Seidel K., Brown C., Gustafson J.A., Yokoyama J.K., Johnson A.J., Baxter B.A., Koning R.W., Reid A.N., Meechan M., Biery M.C., Myers C., Rawlings-Rhea S.D., Albert C.M., Browd S.R., Hauptman J.S., Lee A., Ojemann J.G., Berens M.E., Dun M.D., Foster J.B., Crotty E.E., Leary S.E., Cole B.L., Perez F.A., Wright J.N., Orentas R.J., Chour T., Newell E.W., Whiteaker J.R., Zhao L., Paulovich A.G., Pinto N., Gust J., Gardner R.A., Jensen M.C, & Park J.R. (2022). Intraventricular B7-H3 CAR T Cells for Diffuse Intrinsic Pontine Glioma: Preliminary First-in-Human Bioactivity and Safety. Cancer Discovery, 13(1), 114-131.
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4

Cytokine Secretion Assay for CAR T Cells

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To investigate cytokine secretion, B7-H3 CAR CD8+ T cells and target cells were plated at a 2:1 ratio and the supernatant was analyzed for IL2, IFNγ, and TNFα production after 24-hour incubation using the Bio-Plex multiplex bead array system (Bio-Rad). In vitro cytotoxicity and cytokine release assays were performed in triplicate and repeated for validity.
Vitanza N.A., Wilson A.L., Huang W., Seidel K., Brown C., Gustafson J.A., Yokoyama J.K., Johnson A.J., Baxter B.A., Koning R.W., Reid A.N., Meechan M., Biery M.C., Myers C., Rawlings-Rhea S.D., Albert C.M., Browd S.R., Hauptman J.S., Lee A., Ojemann J.G., Berens M.E., Dun M.D., Foster J.B., Crotty E.E., Leary S.E., Cole B.L., Perez F.A., Wright J.N., Orentas R.J., Chour T., Newell E.W., Whiteaker J.R., Zhao L., Paulovich A.G., Pinto N., Gust J., Gardner R.A., Jensen M.C, & Park J.R. (2022). Intraventricular B7-H3 CAR T Cells for Diffuse Intrinsic Pontine Glioma: Preliminary First-in-Human Bioactivity and Safety. Cancer Discovery, 13(1), 114-131.
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5

Cytokine Secretion Profiling of CAR T Cells

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To investigate cytokine secretion, CAR T cells and target cells were plated at a 2:1 ratio and incubated for 24 hours. Supernatant was then analyzed for IL-2, IFNg and TNFa production using the Bio-Plex multiplex bead array system (Bio-Rad).
Ravanpay A.C., Gust J., Johnson A.J., Rolczynski L.S., Cecchini M., Chang C.A., Hoglund V.J., Mukherjee R., Vitanza N.A., Orentas R.J, & Jensen M.C. (2019). EGFR806-CAR T cells selectively target a tumor-restricted EGFR epitope in glioblastoma. Oncotarget, 10(66), 7080-7095.
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