Topological features of infected bacteria were imaged in liquid at 25 °C by using an Asylum Research Cypher ES instrument (Oxford Instruments, Abingdon, UK). Sample surfaces were scanned in non-contact mode with Olympus BL-AC40TS cantilevers (8 nm average tip radius) oscillated by BlueDrive technology (photothermal excitation) near their resonance frequencies (~20 kHz) at typical scan speeds of 5 µm/s. Scanning resolutions were typically 512 pixels/line. Image post-processing and analysis were performed within the AFM driving software (IgorPro, WaveMetrics, Portland, OR, USA). Membrane roughness (Rq, root-mean-square roughness) was calculated on curvature-corrected 1 × 1 µm regions of bacterial surfaces, using the built-in function of the AFM driving software. Roughness was calculated by selecting multiple 0.5 × 0.5 µm regions of cell surfaces on at least three cells per fixation timepoints. Regarding the number of phage-to-bacterium binding statistics, n = 9 bacterial cells were analyzed.
Bl ac40ts cantilevers
Manufactured by Olympus
The BL-AC40TS cantilevers are a type of scanning probe microscopy (SPM) probe produced by Olympus. They are designed for use in atomic force microscopy (AFM) applications. The cantilevers have a typical length of 40 microns and are made of silicon.
Automatically generated - may contain errors
4 protocols using bl ac40ts cantilevers
1
Imaging Bacterial Surface Topography
2
Atomic Force Microscopy of Supported Lipid Bilayers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AFM experiments were always done in liquid, imaging was performed at the SPM@ISMN facility in Bologna [27, (link)28] (link) using a Multimode VIII (Bruker, Santa Barbara, CA, US) and at the Partnership for Soft Condensed Matter (PSCM) in Grenoble using a Cypher S (Asylum Research, Santa Barbara, CA, US). In the first case images were collected in peakforce tapping using SNL Bruker cantilevers with nominal spring constant of 0.24 N/m and 2-10 nm curvature radius, in the second one Olympus BL-AC40TS cantilevers were chosen to perform tapping mode imaging. Images were processed with Gwyddion (D Nec ˇas & P Klapetek. ''Gwyddion: an open-source software for SPM data analysis, by simply plane-fitting. SLBs and SLBs + SPIONs were prepared according to the previously described protocol.
3
Atomic Force Microscopy in Liquid
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AFM experiments in liquid were performed at the SPM@ISMN facility in Bologna using a Multimode VIII (Bruker, Santa Barbara, CA, US) and at the Partnership for Soft Condensed Matter (PSCM) in Grenoble using a Cypher S (Asylum Research, Santa Barbara, CA, US). In the first case images were collected in peakforce tapping using SNL Bruker cantilevers with nominal spring constant of 0.24 N/m and 2-10 nm curvature radius, in the second one Olympus BL-AC40TS cantilevers were chosen to perform tapping mode imaging. Details on samples preparation and image analysis are reported in the SI (page S8 of Supplementary Materials and Methods).
4
Atomic Force Microscopy of Vesicles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Vesicle samples were diluted to a total protein content of 1 μg/ml in PBS and a 30 μl drop was deposited on a freshly cleaved mica substrate for 10 min at room temperature. Vesicle images were immediately acquired by using an Asylum Research Cypher Microscope (Oxford Instruments, Abingdon, United Kingdom) operating in liquid tapping mode (resolution 512 × 512, scan rate 0.5 Hz). We used Olympus BL-AC40TS cantilevers with a typical tip curvature radius of 10 nm (nominal spring constant 0.1 N/m, resonance frequency 25 kHz in liquid). The free oscillation amplitude was set at 10 nm and images were acquired at 50% larger of the free amplitude. AFM experiments were performed at the PSCM facility of the European Synchrotron Radiation Facility (Grenoble, France).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!