Solutol hs 15

MY11 (purity 95%) was synthesized and provided by pharmaceutical chemistry laboratory, East China University of Science and Technology, China [23 (link)]. RPMI 1640 and DMEM was purchased from Hyclone (Logan, UT, USA). MEM, Penicillin/Streptomycin and Fetal Bovine Serum (FBS) were obtained from Gibco (Invitrogen Corporation, New York, USA). Antibodies for Cyclin D1 (A19038), PUMA (A3752), Bcl-2 (A19693), Bax (A19684), NF-κB p65 (A19653), Phospho-NF-κB p65-S536 (AP0124), β-Actin (AC004) and secondary antibodies were purchased from ABclonal (Wuhan, China). Caspase-9 (#9502) and p21 (#2947) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). FITC Annexin V Apoptosis Detection Kit were purchased from BD Biosciences Company. SYTOX Red dead cell stain was purchased from Invitrogen. SYBR green master mix was purchased from Vazyme. Solutol HS-15 was obtained from MedChemExpress. Hematoxylin and Eosin Staining Kit were purchased from Beyotime.

RTV, LPV, and indinavir sulfate were purchased from Macklin Inc. (Shanghai, China); testosterone was purchased from Sigma-Aldrich (St. Louis, MO, United States); 6β-hydroxytestosterone was purchased from Toronto Research Chemicals Inc. (Toronto, Canada); β-nicotinamide adenine dinucleotide phosphate (NADPH) was purchased from Roche (Roche, Switzerland); propranolol was obtained from the National Institutes for Food and Drug Control (Beijing, China); Solutol HS-15 was purchased from MedChemExpress LLC (Shanghai, China); and XYPI was purchased from Jiangxi Qingfeng Pharmaceutical Co. Ltd. (Jiangxi, China). A pooled sample of human liver microsomes (HLM, 452161) was obtained from BD Gentest Corporation (BD Gentest TM, Woburn, MA, United States). Antibodies specific for CYP3A1 (Mouse monoclonal antibody) was purchased from Santa Cruz Biotechnology (Cruz, CA, United States). Primary antibody for GAPDH (Rabbit Polyclonal antibody) and the second-antibody specific for Rabbit and Mouse were purchased from Proteintech Group, Inc. Methanol and acetonitrile of high-performance liquid chromatography (HPLC) grade were obtained from Fisher Co. Ltd. (Waltham, MA, United States). Milli-Q (Millford, MA, United States) water was used throughout the study.

Glycycoumarin (purity>98%, Cat# DSTDG010801) was used as a reference compound and was purchased from Lemeitian Medical Technology Co., Ltd. (Chengdu, China). Psoralidin (Cat# 180526), used as an internal standard (IS), was obtained from Ronghe Pharmaceutical Sciences (Shanghai, China). β-Glucuronidase (Type H-1, Cat# SLCH4420) was obtained from Sigma (St. Louis, MO, United States). Solutol HS-15 (Cat# 159186) was obtained from MedChemExpress LLC (Shanghai, China). Anhydrous sodium acetate (Cat# C12095781) was purchased from Macklin Biochemical Technology Co., Ltd. (Shanghai, China). HPLC-grade acetonitrile and methanol were purchased from Fisher Scientific (Emerson, IA, United States). MCI GEL was purchased from Mitsubishi Chemical (Tokyo, Japan). Silica gel was obtained from Qingdao Haiyang Chemical Co., Ltd. (Qingdao, China). Analytical-grade ethyl acetate, methanol, and dichloromethane were obtained from Fuyu Fine Chemical Co., Ltd. (Tianjin, China).