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3 protocols using sulfaguanidine

1

Analytical Method for Sulfonamides

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Sulfadiazine (SDZ), sulfaguanidine (SGD), sulfamethazine (SMZ), sulfamerazine (SMR), sulfamethoxazole (SMO), and fluorescamine were obtained from Sigma Aldrich (St. Louis, MO, USA). HPLC-grade acetonitrile, methanol, and acetone were purchased from Baker (Deventer, The Netherlands). Ethyl acetate, acetic acid (99.5%), and sodium acetate were purchased from Chempur (PiekaryŚląskie, Poland), and the ammonia solution 25% was purchased from POCH (Gliwice, Poland). Purified water was prepared in-house with a Milli-Q water system from Millipore (Bedford, MA, USA).
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2

Quantitative Analysis of Sulfonamides

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Certified standards of sulfaguanidine, sulfadiazine, sulfamerazine, sulfamethazine, sulfamethoxazole, and fluorescamine were obtained from Sigma Aldrich (St. Louis, MO, USA). All reagents used were of analytical grade, >95% purity. Acetonitrile and methanol for HPLC were from J.T. Baker (St. Louis, MO, USA). Ethyl acetate, acetic acid and ammonia solution (25%) were purchased from POCH (Gliwice, Poland). High purity water with a resistivity of 18.2 MΩ cm−1 was obtained from a Milli-Q water system (Millipore, Bedford, MA, USA).
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3

LC-MS/MS Protocol for 3HPMA Quantification

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N-acetyl-S-(3-hydroxypropyl)-L-cysteine sodium salt (3HPMA, CAS: 14369-42-7), with a purity higher than 98%, was obtained from TLC Pharmaceutical Standards (New Market, ON, Canada). Terfenadine and Val-Tyr-Val from Sigma Life Sciences (St. Louis, MO, USA), triallyl phosphite from Alfa Aesar Thermo Fischer (Kandel, Germany) and sulfaguanidine, sulfadimethoxine, reserpine and acetaminophen obtained from Sigma-Aldrich (St. Louis, MO, USA) were employed as internal standards for LC-MS/MS in 3HPMA determination. Methanol (LC-MS grade), acetonitrile (LC-MS quality) and buffer constituents, acetic acid and ammonium formate, were provided by VWR Chemicals (Radnor, PA, USA) and Scharlau (Barcelona, Spain). Ultrapure water with a maximum resistivity of 18.2 MU cm -1 from an Adrona B-30 Bio system (Adrona, Riga, Latvia) was used.
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